ISSN:
1432-1424
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary Intracellular potentials were measured, using a piezoelectric electromechanical transducer to impale Ehrlich ascites tumor cells with capillary microelectrodes. In sodium Ringer's, the potential immediately after the penetration was −24±7 mV, and decayed to a stable value of about −8 mV within a few msec. The peak potentials disappeared in potassium Ringer's and reappeared immediately after resuspension in sodium. Ringer's, whereas the stable potentials were only slightly influenced by the change of medium. The peak potential is in good agreement with the Nernst potential for chloride. This is also the case when cell sodium and potassium have been changed by addition of ouabain. It is concluded that the peak potentials represent the membrane potential of the unperturbed cell, and that chloride is in electrochemical equilibrium across the cell membrane. The membrane potential of about −11 mV previously reported corresponds to the stable potential in this study, and is considered as a junction potential between damaged cells and their environment. Similar potential differences were recorded between a homogenate of cells and Ringer's. The apparent membrane resistance of Ehrlich cells was about 70 Ωcm2. This is two orders of magnitude less than the value calculated from36Cl fluxes, and may, in part, represent a leak in the cell membrane. For comparison, the influence of an eventual leak on measurements in red cells and mitochondria is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02116574
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