ISSN:
1435-1536
Keywords:
Soluble complex
;
acid polysaccharide
;
bovine serum albumin
;
electrophoretic mobility
;
dynamic light scattering
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
,
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Abstract Soluble complexes between sodium hyaluronate (NaHA) and bovine serum albumin (BSA) were studied by dynamic light scattering DLS and an electrophoretic light scattering ELS. Various amounts of BSA were added to NaHA solution (1.2 μmol dm−3=0.1 w/v%) and their electrical mobilityU were measured underJ=0.001 mol dm−3. Their spectra ofU were found to be one sharp peak in the experimental concentration region (the maximum concentration:C BSA=128 μmol dm−3). The value ofU attained to a constant negative value in the region ofC BSA〉120 μmol dm−3 in which a saturated soluble complex was found to be formed. From the results of viscosity and diffusion coefficients of the mixed solutions, with increasingC BSA the shape of complexes was found to change from a worm-like to random-coil structure. The ratio of BSA to NaHA of the saturated complex was 120 and 18 repeating units bind to one BSA molecule, i.e., 18 carboxylic groups participate in the binding.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01189532
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