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  • 1
    Electronic Resource
    Electronic Resource
    Improvement of Bypass Circuit Biocompatibility: Comparison and Combination of Heparin-Coated Circuit and Nitric Oxide Gas Infusion (2002)
    Malden, USA : Blackwell Science Inc
    Journal of cardiac surgery 17 (2002), S. 0 
    Details
    ISSN: 1540-8191
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract Objectives: Nitric oxide (NO) gas infusion to the oxygenator, as well as heparin-coated bypass circuits, have been reported to attenuate blood activation induced by the interaction with the artificial surfaces of an extracorporeal bypass circuit. Using a mock circulation model, we compared the effect of each and also evaluated the effect of their combination on attenuating bypass-induced blood activation. Methods: A miniature closed bypass circuit was primed with diluted fresh human blood and perfused for 180 minutes using a centrifugal pump. NO gas (0, 50, or 100 ppm) was infused to the oxygenator sweep gas of either a non-heparin-coated or a heparin-coated circuit. Platelet counts, β-thromboglobulin, platelet factor 4, complement-3 activation products and granulocyte elastase were measured at 0, 30, 60, 120, and 180 minutes after starting the perfusion. Results: One hundred ppm of NO was statistically equivalent to the heparin-coated circuit for attenuating bypass-induced blood activation, and a combination of the two significantly surpassed the results of either modification alone. Fifty ppm of NO alone provided only a slight attenuation of blood activation as compared with the non-heparin-coated circuit, though the difference was not significant. A combination of 50 ppm NO and the heparin-coated circuit did not significantly enhance the effects of the heparin-coated circuit alone. Conclusions: The combination of NO gas infusion and heparin-coated circuits appears to be a useful and promising modification for enhancing the attenuation of bypass-induced blood activation, though the optimal dose of NO infusion in terms of effectiveness and adverse effects to the whole body remains to be established.(J Card Surg 2002;17:477-484)
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1540-8191.2002.01001.x
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  • 2
    Electronic Resource
    Electronic Resource
    Expression of mRNA encoding IFNα in macrophages stimulated with Lactobacillus gasseri (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 120 (1994), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The ability of Lactobacillus gasseri, a dairy lactic acid bacterium, to induce interferon (IFN) was investigated in murine macrophage cultures. IFNα was substantially induced by some strais of L. gasseri and the titers were the highest at a concentration of 100 μg ml−1 of L. gasseri DSM20243T. The expression of mRNA encoding IFNα was detected in spleen-macrophages (SP-Mθ) and Peyer's patch-adherent cells stimulated with L. gasseri DSM20243T. Actinomycin D and cycloheximide added to SP-Mθ cultures showed that the mRNA was synthesized by 0.5 h, and that IFNα was produced within 3 to 6 h after the stimulation with L. gasseri DSM20243T. The results support the notion that dairy products containing L. gasseri can be ‘physiologically functional foods’.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1994.tb07052.x
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  • 3
    Electronic Resource
    Electronic Resource
    Sequence of IGF-I, IGF-II, and HGF expression in regenerating skeletal muscle (2000)
    Springer
    Histochemistry and cell biology 122 (2000), S. 427-434 
    Details
    ISSN: 1432-119X
    Keywords: Bovine ; Skeletal muscle ; HGF ; IGF-I ; IGF-II
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: AbstractVarious cytokines are thought to play a role in muscle regeneration, however, the interaction and mechanisms of action of these cytokines remains largely unknown. In this study, we investigated the role of HGF, IGF-I, and IGF-II during myogenesis using the regeneration model of skeletal muscle as well as myoblast culture. RT-PCR analysis revealed that HGF and IGF-I expressions were markedly upregulated, in regenerating muscle. In contrast, there was no significant difference in IGF-II expression between normal and regenerating muscle. Immunohistochemical analysis demonstrated that HGF was expressed mostly by myocytes during the early stages of muscle regeneration. Additionally, HGF inhibited the formation of myotubes by myoblasts, but promoted cellular proliferation. Otherwise, IGF-I and IGF-II were expressed by myocytes through the early to middle stages of muscle regeneration. The addition of HGF to myoblast growing in vitro significantly increased the number of cells. These findings indicate that these three cytokines have pleiotropic effects in regenerating skeletal muscle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s00418-004-0704-y
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  • 4
    Electronic Resource
    Electronic Resource
    A dioxygenase gene (Ids2) expressed under iron deficiency conditions in the roots of Hordeum vulgare (1994)
    Springer
    Plant molecular biology 25 (1994), S. 705-719 
    Details
    ISSN: 1573-5028
    Keywords: chlorosis ; DNA sequence ; Ids2 ; iron deficiency ; MAs ; phytosiderophores
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A λzapII cDNA library was constructed from mRNA isolated from Fe-deficient barley roots and screened with cDNA probes made from mRNA of Fe-deficient and Fe-sufficient (control) barley roots. Seven clones were selected. Among them a clone having the putative full-length mRNA of dioxygenase as judged by northern hybridization was selected and named Ids2 (iron deficiency-specific clone 2). Using a cDNA fragment as probe, two clones from the genomic library (λEMBL-III) were isolated and one was sequenced. The predicted amino acid sequence of Ids2 resembled that of 2-oxoglutarate-dependent dioxygenase. Ids2 is expressed in the Fe-deficient barley roots but is not in the leaves. The expression is repressed by the availability of Fe. Ids2 was also strongly expressed under Mn deficiency and weakly under Zn deficiency or excess NaCl (0.5%). The upstream 5′-flanking region of Ids2 has a root-specific cis element of the CaMV 35S promoter and a nodule-specific element of leghemoglobin, a metal regulatory element (MRE) and several Cu regulatory elements (UAS) of yeast metallothionein (CUP1).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00029608
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  • 5
    Electronic Resource
    Electronic Resource
    Role of nitric oxide in regulation of systemic vascular resistance during and after cardiopulmonary bypass (1999)
    Springer
    Journal of artificial organs 2 (1999), S. 152-156 
    Details
    ISSN: 1619-0904
    Keywords: Nitric oxide ; Cardiopulmonary bypass ; Vasoactive substance ; Systemic vascular resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract Cardiopulmonary bypass (CPB) is known to result in the abnormal production of vasoactive substances contributing to the changes in hemodynamics such as systemic vascular resistance (SVR) during and after CPB. Nitric oxide (NO) is an inflammation-mediated vasoactive substance that plays a role in the whole-body inflammatory response induced by CPB. We evaluated the role of NO in the regulation of SVR during and after CPB. Fifteen patients underwent open-heart surgery for valvular heart disease. The perfusate blood temperature of CPB was set to 34°C. The plasma levels of NO metabolites (NO 2 − +NO 3 − ), prostaglandin E2 (PGE2), bradykinin (BK), and systemic vascular resistance index (SVRI) were measured before CPB and 0, 12, and 24 h after CPB. The plasma level of NO metabolites increased gradually after CPB (pre-CPB, 26.3 ±4.4; 0h, 33.7±6.5; 12 h, 49.8±11.1; 24 h, 43.1±7.5 μM). SVRI decreased gradually after CPB (pre-CPB, 2361±364; 0h, 2048±216; 12 h, 1590±308; 24 h, 1727±435 dyne·s·cm−5·m2). There was a significant inverse correlation between SVRI and the plasma level of NO metabolites as a whole (r=−0.674,P〈0.0001). No significant correlations were observed between SVRI and the other vasoactive substances PGE2 and BK. These findings demonstrated that NO production increased gradually during and after CPB in association with the decrease in SVR.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02480059
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  • 6
    Electronic Resource
    Electronic Resource
    Percutaneous cardiopulmonary support system as a bridge to left ventricular assist system (LVAS) implantation: Analysis of its role in the treatment of end-stage cardiac disease (1998)
    Springer
    Journal of artificial organs 1 (1998), S. 33-36 
    Details
    ISSN: 1619-0904
    Keywords: PCPS ; LVAS ; Heart failure ; Multiple organ failure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract The key to the successful implantation of a left ventricular assist system (LVAS) for patients with endstage cardiac disease is whether the functions of other vital organs are irreversibly damaged or not. The percutaneous cardiopulmonary support system (PCPS) is not only equal in convenience to the intra-aortic balloon pump (IABP), it is more powerful in resuscitating impaired end-organ function. To investigate the efficacy of PCPS for end-state cardiac disease, we retrospectively analyzed end-organ function before and after the application of PCPS. From 1992 to 1996, five cardiomyopathy patients with deteriorated end-organ function despite the application of IABP underwent PCPS support before implantation of LVAS. Urine volume and levels of liver enzymes (sAST and sALT) and serum creatinine were determined before and after the application of PCPS. After the application of PCPS, the urine output increased significantly (1840±450 to 4340±470 ml/day,P〈0.01) and levels of sAST, sALT, and serum creatinine decreased significantly (630±220 to 150±50IU/l, 630±260 to 260±130IU/l, and 2.9±0.5 to 1.2±0.1 mg/dl, respectively) (P〈0.05). All five patients were successfully bridged to LVAS implantation and none of them died of multiple organ failure caused by pre-existing cardiac failure although one out of five patients died on device ultimately. These results indicated that PCPS before LVAS implantation is useful to resuscitate impaired end-organ function and to improve the survival rate of LVAS implantation for end-stage cardiac disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01340450
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  • 7
    Electronic Resource
    Electronic Resource
    The “double grafted tumor system”, proposed to find effector cells in the analyses of antitumor effect of BRMs (1989)
    Springer
    Biotherapy 1 (1989), S. 139-151 
    Details
    ISSN: 1573-8280
    Keywords: adoptive transfer ; antitumor effect ; BRM (biological response modifier)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The antitumor effects of three biological response modifiers (BRMs; PSK, IFNα A/D and OK432) and two chemotherapeutics (Mitomycin C and Neocarzinostatin) in a new experimental mouse model, the “double grafted tumor system,” were evaluated. BALB/c mice received simultaneous inoculations of Meth A fibrosarcoma cells on right flank (1 × 106 cells) and left flank (2 × 105 cells) on day 0, and drugs were given intratumorally into the right-flank tumor on day 3. The growth of the left-flank tumor was the real target for the evaluation of a given drug after 21 days. All tested five agents successfully cured the drug-injected right tumor with a pre-determined optimum dose. In addition, PSK, OK432, IFNα A/D and MMC among the five, inhibited the left-flank tumor, whereas no inhibition was observed when treated with NCS. To understand the mechanism by which the antitumor effect of the above four agents is able to influence the growth of tumor on the other side, tumor cells (2 × 105 cells) inoculated only into the left flank were treated with drugs given subcutaneously to the right flank (single tumor system). Among the four, MMC exhibited an effect similar to that obtained in the double tumor system, and IFNα A/D showed a less pronounced but still definite antitumor effect. However, PSK and OK432 failed to express anti-tumor effect in the single tumor system. These results obtained with PSK, OK432 and IFNαA/D suggest that the effect of the drug on the left-tumor may be mediated by certain effector cells, which are specifically induced by injection of the drug, in the right-tumor tissues. When effector cell analysis was conducted with spleen cells obtained after PSK treatment by means of intratumoral adoptive transfer into 3-day Meth A bearing recipients, these cells were shown to be Lyt-1+2−-T and L3T4+-T cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02170883
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