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  • 1
    Electronic Resource
    Electronic Resource
    A Rapid Method for the Separation of Functional Lymphoid Cell Populations of Human and Animal Origin on PVP-Silica (Percoll) Density Gradients (1979)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 10 (1979), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Subclasses of lymphocytes can be separated on gradients of non-toxic polyvinylpyrrolidone-coated colloidal silica (Percoll) by virtue of differential densities. Such gradients can yield functionally active lymphocyte populations after brief centrifugation. Gradients can he generated in a discontinuous step fashion and centrifuged in standard table-top laboratory centrifuges or as self-generating gradients duriny ultracentrifugation. The density medium has low viscosity and can be made isotonic for virtually any use. Gradients have proved useful in both human and experimental animal studies, and high percentage yields allow for separations from small cell numbers. Methods are described for separation of whole blood and lymphotd subpopulations. including blasts stimulated with mitogens or in mixed lymphocyte reactions. The cytotoxic capability of various density fractions was evaluated for mixed lymphocyte culture-induced allogeneic killing and spontaneous, so-called ‘natural’ killer cell activity. The lower density associated with blast transformation allows for significant enrichments of stimulated cells from in vitro cultures. Higher thymidine incorporation, restimulation in mixed lymphocyte reactions, and greater cytotoxic capacity are associated with these‘blast’fractions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1979.tb01391.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Reactions and Crossreactions of a Rabbit Anti-H2 Antigen Serum (1978)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 7 (1978), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A rabbit antiserum raised against highly purified, papain-solubilized H-2d antigens contained two sets of non-crossreacting antibodies directed against each one of the two H-2 antigen sub-units. The antiserum recognized only 12,000 and 47,000 dalton polypeptide chains when spleno-cyte membrane glycoproteins were analysed. Among the molecules precipitated with the rabbit antiserum all H-2K and D antigens were present. In addition to regular H-2K and D antigens minor amounts of material with the typical H-2 antigen subunit structure, but lacking alloantigenic determinants, were precipitated by the antiserum. These ‘non-H-2 antigens’ were produced in relatively greater amounts by T-cells than by B-cells. Both sets of antibodies in the rabbit antiserum reacted with the TL antigens demonstrating that there is an immunological crossreactivity between the classical alloantigenic H-2 antigen chain and the alloantigenic TL antigen chain. The F9 cell line, believed to represent cells at the morula stage, display H-2 antigen-like structures as revealed by the rabbit antiserum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1978.tb00454.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Entrapment of animal cells for production of monoclonal antibodies and other biomolecules (1983)
    [s.l.] : Nature Publishing Group
    Nature 302 (1983), S. 629-630 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Previous studies had demonstrated that animal cells can be entrapped in a viable state in alginate beads, providing preparations in which the cells are either embedded throughout the network of the bead3 or kept within the microcapsules, which are characterized by a shell of ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/302629a0
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    Paper (German National Licenses)
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