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PROSTAGLANDIN SYNTHESIS IN THE ENDOMETRIUM OF WOMEN WITH OVULAR DYSFUNCTIONAL UTERINE BLEEDING (1981)

Smith, S. K. ; Abel, M. H. ; Kelly, R. W. ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

BJOG 88 (1981), S. 0

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ISSN: 1471-0528

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: The endogenous concentrations of prostaglandins F2α (PGF2α) and E (PGE) were measured during the luteal phase of the menstrual cycle in the endometrium from 14 women with unexplained menorrhagia (measured menstrual blood loss in excess of 50 ml) and 15 women with normal menses (blood loss 50 ml or less). Although there was no significant difference in the PGF 2α/PGE ratio between the two groups, this ratio was significantly lower in the endometrium from eight of the women whose blood loss exceeded 90 ml (p 〈0.05). There was a significant inverse correlation between the PGF2α/PGE ratio and blood loss (r = 0.36, p 〈0.025). The synthetic capacity of the endometrium was assessed by incubation of the tissue with 14C arachidonic acid. Endometria from nine women with unexplained menorrhagia synthesized more PGE2 than PGF2α, whereas the converse was true with 11 control endometria. Consequently the PGF2α/PGE2 ratio was significantly reduced in the former group (p 〈0.025). Oestradiol-17β (200 μM) and to a greater extent 2 hydroxy oestradiol (200 μM) increased the total prostaglandin synthesis by the endometria, but did not significantly alter the PGF2α/PGE2 ratio. These results suggest that excessive blood loss may be associated with a shift in the endometrial conversion of prostaglandin endoperoxide from PGF2α to PGE2.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1471-0528.1981.tb01009.x

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The measurement of 13,14-dihydro-15-keto prostaglandin E2 by combined gas chromatography mass spectrometry (1983)

Kelly, R. W. ; Abel, M. H.

Chichester : Wiley-Blackwell

Biological Mass Spectrometry 10 (1983), S. 276-279

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ISSN: 0306-042X

Keywords: Chemistry ; Analytical Chemistry and Spectroscopy

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: The main metabolite of prostaglandin E2 (PGE2) 13,14-dihydro-15-keto-PGE2 readily dehydrates and can subsequently form a cyclic derivative. This problem can be overcome by the immediate formation of oximes of the 9 and 15 ketones in aqueous solution followed by subsequent extraction, methylation and t-butyldimethyl silylation of the free hydroxyl groups at 11 and on the oximes. Deuterogenated 13,14-dihydro-15-keto-PGE2 is used as the internal standard and can be stored as the oxime and added with the oximating solution immediately the biological sample is obtained. The sensitivity of the method allows measurement of 2 ng of 13,14-dihydro-15-keto-PGE2 in tissue incubates. The intra-batch precision is 11.8% and the inter-batch precision for measurement of 100 ng of metabolite is 8.1%.

Additional Material: 3 Ill.