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  • 1
    Electronic Resource
    Electronic Resource
    Interaction between supernodulating or non-nodulating mutants of soybean and two arbuscular mycorrhizal fungi (2000)
    Springer
    Mycorrhiza 10 (2000), S. 101-106 
    Details
    ISSN: 1432-1890
    Keywords: Keywords Arbuscular mycorrhiza ; Mycorrhizal inoculation effect ; Nodulation mutants ; Soybean ; Supernodulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Twelve nodulation mutants (seven non-nodulating and five supernodulating) of soybean [Glycine max (L.) Mirr.] were screened for arbuscular mycorrhizal colonization in the presence of either Glomus etunicatum Becker and Gerdemann or Gigaspora margarita Becker and Hall. The cultivars showed variation in colonization parameters. The two supernodulating mutants En6500 and NOD1–3 had higher frequencies of colonization with 2.5–4.5 times higher arbuscular abundance than the respective wild types. The enhanced mycorrhization resulted in significant enhancement of P uptake by En6500. The non-nodulating mutants showed decreases in mycorrhizal parameters. Mutants En1282 and Harosoy–exhibited aborted infection after formation of typical appressorium-like structures at some sites. However, none of these had the non-mycorrhizal phenotype. Growth and nutrient-uptake parameters should be considered while studying plant mutants for mycorrhization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s005720000064
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The use of GUS-reporter gene to study the effect of Azospirillum-Rhizobium coinoculation on nodulation of white clover (1998)
    Springer
    Biology and fertility of soils 27 (1998), S. 349-352 
    Details
    ISSN: 1432-0789
    Keywords: Key words Acetylene reduction activity ; Azospirillum-Rhizobium coinoculation ; GUS-reporter gene ; Nodulation ; White clover
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  The gusA-marked Azospirillum lipoferum T1371, constructed by inserting transposon mTn5SSgusA20 from Escherichia coli S17-1 λ-pir into the genome of Azospirillum lipoferum 137, was used to evaluate its effect on nodulation of white clover with and without Rhizobium inoculation. When inoculated alone, Azospirillum colonized the tap roots, secondary roots and root hairs. The combined inoculation of white clover with Rhizobium leguminosarum bv trifolii and A. lipoferum enhanced the number of nodules by 2–3 times from 5 to 20 days after inoculation (DAI). The combined inoculation also enhanced acetylene reduction activity by 2.3–2.7 times at 20 DAI. Moreover, Azospirillum was observed colonizing the tap root, root hairs and sites near or on the nodules. These results suggest that the formation of additional infection sites by A. lipoferum, with a combined inoculation, may be the mechanism that will enhance nodulation and nitrogen fixation of white clover.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003740050442
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Aminopeptidase activity in the brains of mice with chronicToxoplasma gondii infections (1989)
    Springer
    Parasitology research 75 (1989), S. 186-188 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Aminopeptidase activity on β-naphthylamide (NA) substrates was assayed in brain extracts from normal andToxoplasma gondii-infected mice at 4 months postinfection. Correlations of levels of aminopeptidase activity,Toxoplasma-specific antibody production, and the number of brain cysts were studied in normal andToxoplasma-infected mice. TheToxoplasma-specific antibody and the formation of cysts were markedly enhanced in the parasite-infected mice. The highest levels of activity for the NA substrates tested were observed in normal mice. In contrast, the activity levels were significantly lower inT. gondii-infected mice than in the corresponding normal mice. These results suggest an association between chronic toxoplasmosis and aminopeptidase activity in the parasite-infected host brain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00931273
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Histochemical studies on pseudocysts in adenoid cystic carcinoma of the human salivary gland (1985)
    Springer
    Journal of molecular histology 17 (1985), S. 913-924 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Pseudocysts are unique structures found in adenoid cystic carcinomata of human salivary glands. They were studied in 13 such cases by histochemical and immunohistochemical means. The pseudocysts contained an abundance of mucoid materials which reacted strongly with both Alcian Blue and dialysed iron ferrocyanide. The mucoid material was digested with chondroitinase ABC and heparitinase, but was resistant toStreptomyces hyaluronidase. The inner surfaces of the pseudocysts were strongly reactive for laminin, whereas the interface between the tumour cell nests and the outer stromal area was intensely reactive for fibronectin. Numerous fibronectin-reactive fibrils and blood coagulation factor XIII (F-XIII)-positive cells were distributed extensively in the outer stromal area. The F-XIII-positive cells were also found within some pseudocysts. The results obtained in the present study have shown that the pseudocysts represent a peculiar structure consisting of basement membrane components; laminin, fibronectin, heparan sulphate and chondroitin sulphate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01004186
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    Paper (German National Licenses)
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