ISSN:
1573-8221
Keywords:
schirrous carcinoma of the human stomach
;
culture in diffusion chambers
;
thymidine-H3
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract When a schirrous carcinoma of the human stomach was cultured in diffusion chambers, growth of epithelial and connective-tissue cells was observed. Unlike the original tumor tissue in vitro when the connective-tissue cells did not incorporate thymidine-H3, during growth in diffusion chambers they were labeled. The fraction of cells with labeled nuclei 1 h after injection of thymidine-H3 in the zone of growth averaged 25.1%, much greater than the proportion of labeled cells determined after incubation of the original tumor tissue in vitro (6.6%). Under the conditions of culture, a rapidly proliferating subpopulation of cells with a mitotic cycle 14.8 h in duration was discovered.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00786431
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