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  • 1
    Electronic Resource
    Electronic Resource
    Phytase production and decrease of phytic acid content in canola meal byAspergillus carbonarius in solid-state fermentation (1995)
    Springer
    World journal of microbiology and biotechnology 11 (1995), S. 228-231 
    Details
    ISSN: 1573-0972
    Keywords: Aspergillus carbonarius ; canola meal ; phytase ; phytic acid ; solid-state fermentation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Solid-state fermentation (SSF) usingAspergillus carbonarius with canola meal as a substrate showed that production of phytase was associated with growth; maximum activity was achieved after 72 h. Apparent 25% and 10% increases in the protein content of the canola meal were noticed after 48 h and 72 h, respectively but total carbohydrate concentration had fallen by 25% by the end of fermentation. The rate of decrease of phytic acid content was optimum with a moisture content between 53% and 60%; homogenization of the inoculum for 120 s led to the greatest biomass and lowest phytic acid content. Inoculation of sterile meal led to lower phytic acid contents than inoculation of non-sterile meal.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00704655
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  • 2
    Electronic Resource
    Electronic Resource
    The effect of phosphate concentration on phytase production and the reduction of phytic acid content in canola meal by Aspergillus carbonarius during a solid-state fermentation process (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 25-30 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The use of canola meal, an abundant side-product of canola oil processing in Canada, as animal feed is hampered by high phytic acid levels that reduce metal cation availability. Aspergillus carbonarius grows well in a solid canola meal medium, produces phytase and reduces the phytic acid content to zero. Inorganic phosphate addition at a concentration of 1 mg and 5 mg/110 g solid-state culture system results in better growth of the microorganism, higher rates and levels of phytase production, and faster reduction of phytic acid content. Phosphate concentrations of 50mg and 100 mg/110 g inoculated system had a negative effect affecting primarily the initial rates of biomass and phytase production and phytic acid content reduction. Models that predict biomass production (expressed as glucosamine content) and phytase, as well as the reduction of phytic acid content in the solid-state cultures supplemented with phosphate are reported. They fit the experimental results reasonably well (with a maximum deviation of 7%).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00170617
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  • 3
    Electronic Resource
    Electronic Resource
    The effect of surfactants on the phytase production and the reduction of the phytic acid content in canola meal byAspergillus carbonarius during a solid state fermentation process (1994)
    Springer
    Biotechnology letters 16 (1994), S. 183-188 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary During the growth of A. carbonarius, the rates of biomass growth, phytase production and phytic acid content reduction in canola meal media during solid state fermentation were higher in the presence of Na-oleate or Tween-80 than in the control medium which was not supplemented with these surfactants. Addition of Triton X-100 had a negative effect on the studied processes. The optimum concentration of Na-oleate in solid state culture media was 1%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01021668
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  • 4
    Electronic Resource
    Electronic Resource
    Characteristics of phytase produced by Aspergillus carbonarius NRC 401121 in canola meal (1994)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 14 (1994), S. 223-233 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Aspergillus carbonarius NRC 401121 phytase was produced on canola meal in a solid-state fermentation process. A Km value of 0.345 mM and a vmax of 0.81 units were determined for sodium phytate. The optimum pH and temperature were 4.7 and 53°C, respectively. Activation of the enzyme occurred when it was preincubated at higher temperatures for a period of time. The energy of activation, the entropy and the enthalpy changes were evaluated to be 7,800 cal/mole, 74 cal/(mole · K) and 24,000 cal/mole for this enzyme, respectively.The effect of time and the extractant: solid state culture ratio upon the single step extraction of phytase from a solid-state culture were evaluated.Mathematical correlations which fit the experimental data reasonably well were proposed for some of the studied processes.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370140302
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    Paper (German National Licenses)
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