ISSN:
0138-4988
Keywords:
Life Sciences
;
Life Sciences (general)
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Aspergillus carbonarius NRC 401121 phytase was produced on canola meal in a solid-state fermentation process. A Km value of 0.345 mM and a vmax of 0.81 units were determined for sodium phytate. The optimum pH and temperature were 4.7 and 53°C, respectively. Activation of the enzyme occurred when it was preincubated at higher temperatures for a period of time. The energy of activation, the entropy and the enthalpy changes were evaluated to be 7,800 cal/mole, 74 cal/(mole · K) and 24,000 cal/mole for this enzyme, respectively.The effect of time and the extractant: solid state culture ratio upon the single step extraction of phytase from a solid-state culture were evaluated.Mathematical correlations which fit the experimental data reasonably well were proposed for some of the studied processes.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/abio.370140302
