ISSN:
1432-0789
Keywords:
Dimethylsulfoxide reduction
;
Microbial activity determination
;
Soil aggregates
;
Arginine ammonification
;
Microbial biomass
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Geosciences
,
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Summary Based on the reduction of dimethylusulfoxide (DMSO) to dimethylsulfide (DMS) by microorganisms, a simple, rapid, sensitive and inexpensive method for the determination of microbial activity in soil samples was developed. When DMSO was added to samples, DMS appeared immediately in the gas phase, which was quantitatively analyzed by gas chromatography. The DMS liberation rate was constant for several hours. The reaction immediately starts and its linearity indicate that neither the physiological state nor the number of organisms were changed by the assay. DMSO reduction is widespread among microorganisms; out of 144 strains tested (both fungi and bacteria) only 5 were unable to carry out this reaction. The reaction in soil samples was strongly inhibited by toluene, cyanide, azide, or by fumigation, but was considerably stimulated by glucose. These findings demonstrate that the reaction was due to the activity of microorganisms. The DMSO reduction in different soil samples was significantly correlated with arginine ammonification and heat output (r〉0.9). A good correlation was observed with the organic-matter content (r = 0.74), but not with microbial numbers, clay content, or the pH of the soil. Standard deviations of less than 10% were routinely found. Furthermore, the method is sufficiently sensitive to allow measurements of activity in very small samples (〈 0.1 g). For example, a microbial activity profile can be established for a single soil aggregate, revealing marked differences in activity on the outside and in the interior.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00263168
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