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  • 1
    Electronic Resource
    Electronic Resource
    Functional and molecular evidence for Na+-HCO3 – cotransporter in human corneal endothelial cells (1999)
    Springer
    Pflügers Archiv 438 (1999), S. 458-462 
    Details
    ISSN: 1432-2013
    Keywords: BCECF Cell pH Corneal endothelial cells Na+-HCO3– cotransporter RT-PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Although bicarbonate transport in corneal endothelium has been suggested to be coupled to Na+, the underlying molecular mechanism has not been clarified. In the present study we investigated whether a recently cloned Na+-HCO3 – cotransporter (NBC-1) is responsible for this process, and, if so, whether the endothelium expresses a separate isoform or one of the other two isoforms that have recently been identified (kNBC-1 from kidney and pNBC-1 from pancreas). Using primers designed for specific and common regions we demonstrated by reverse transcriptase polymerase chain reaction (RT-PCR) that both kNBC-1 and pNBC-1 are expressed in cultured human corneal endothelial cells. In addition functional studies with a pH-sensitive fluorescence probe were performed. In the presence of HCO3 –/CO2 a pH regulatory process was demonstrated which depends on the presence of Na+ and membrane potential, but is independent of Cl– and is inhibited by the disulfonic stilbene DIDS. These results support the presence of NBC-1 as the major bicarbonate transport system in corneal endothelium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004249900081
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  • 2
    Electronic Resource
    Electronic Resource
    Study of fluorescein glucuronide (1986)
    Springer
    Graefe's archive for clinical and experimental ophthalmology 224 (1986), S. 110-112 
    Details
    ISSN: 1435-702X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A method for isolating fluorescein glucuronide from the urine using silica gel chromatography and preparative high-performance liquid chromatography is described. The product obtained was free from significant fluorescent impurity on thin-layer chromatography and analytical high-performance liquid chromatography.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02141480
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Study of fluorescein glucuronide (1986)
    Springer
    Graefe's archive for clinical and experimental ophthalmology 224 (1986), S. 113-117 
    Details
    ISSN: 1435-702X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Comparative studies of fluorescein and fluorescein glucuronide were carried out. Binding to human serum protein was studied using an Amicon MPS-3 ultrafiltration unit; it averaged 63% for fluorescein glucuronide and 85% for fluorescein. Intracameral penetration of both compounds was studied in the human eye, and the concentration changes of both compounds in the plasma ultrafiltrate and in the anterior chamber were analyzed, based on Davson's equation. The coefficient of entry into the anterior chamber (k i ) was 0.018 ± 0.007 h−1 (mean ± SD,n = 10) for fluorescein glucuronide and 0.054 ± 0.033 h−1 for fluorescein, and the former was significantly lower than the latter (P 〈 0.005). The rate of loss from the vitreous (k v ) was studied by injecting each compund into the vitreous of the pigmented rabbit and following the fluorescein intensity changes in it. It was 0.042 ± 0.008 h−1 (mean ± SD,n = 8) for fluorescein glucuronide and 0.17 ± 0.01 h−1 for fluorescein, and the former was significantly smaller than the latter (P 〈 0.001). Intraperitoneal injection of probenecid significantly decreased thek v of fluorescein but had little effection that of fluorescein glucuronide. It was suggested that fluorescein glucuronide is lost from the vitreous mainly by a passive mechanism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02141481
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  • 4
    Electronic Resource
    Electronic Resource
    A fluorophotometric study of the corneal graft: A postoperative follow-up (1985)
    Springer
    Graefe's archive for clinical and experimental ophthalmology 222 (1985), S. 263-265 
    Details
    ISSN: 1435-702X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In 59 patients (94 eyes) who underwent penetrating keratoplasty, the aqueous-cornea transfer coefficient,k c ⋅ ac, and the distribution ratio between the aqueous and the cornea,r ac, of fluorescein were determined by means of the oral fluorescein method. The thickness of the graft was also measured. In the early postoperative period,k c ⋅ ac was significantly increased and the graft was thick. During the next several months, both parameters became almost normal. A significantly positive correlation was found betweenk c ⋅ ac and graft thickness. On the other hand, no significant correlation was found betweenr ac and postoperative period,k c ⋅ ac or graft thickness. It was concluded that the endothelial barrier function was damaged at the time of surgery and was gradually repaired postoperatively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02133694
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  • 5
    Electronic Resource
    Electronic Resource
    Effects of S-596 and carteolol, new beta-adrenergic blockers, and flurbiprofen on the human eye: A fluorophotometric study (1985)
    Springer
    Graefe's archive for clinical and experimental ophthalmology 222 (1985), S. 259-262 
    Details
    ISSN: 1435-702X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The effects of S-596 and coarteolol, new beta-adrenergic blockers, were studied by means of the oral fluorescein method. Transfer coefficients by diffusion,k d ⋅ pa, and by flow,k fa, were estimated in a total of 11 normal young volunteers, in whom the drug was instilled in one eye and the placebo in the fellow eye. With 0.5% dl-S-596,k d ⋅ pa increased to 124 ± 9% (mean ± SD) andk fa decreased to 67 ± 10% of the control eye. With 1% dl-carteolol,k d ⋅ pa increased to 121 ± 4%, andk fa decreased to 84 ± 11% of the control eye. Both S-596 and carteolol increased iris permeability and decreased the aqueous flow rate significantly. In a group of 13 cases, intracapsular extraction of senile cataract was carried out with postoperative routine instillations, and in another group of 7 cases, it was carried out with additional topical flurbiprofen, a new nonsteroidal anti-inflammatory drug. Flurbiprofen ophthalmic solution (0.1%) was instilled 3, 2, 1, and 0.5 h before the surgery and 4 times a day postoperatively. On the 6th postoperative day, a fluorophotometric study was carried out using oral fluorescein, and a coefficient that reflects the permeability of the blood-aqueous barrier after the cataract surgery was calculated. It was found that additional topical flurbiprofen considerably suppresses the disruption of the blood-aqueous barrier.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02133693
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  • 6
    Electronic Resource
    Electronic Resource
    Conditions for balance between lower normal pressure control and hypotony in mitomycin trabeculectomy (1998)
    Springer
    Graefe's archive for clinical and experimental ophthalmology 236 (1998), S. 420-425 
    Details
    ISSN: 1435-702X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  · Background: Striving for low to ultra-low postoperative intraocular pressure (IOP) through filtering surgery usually increases the incidence of prolonged hypotony. Early postoperative prognostic indicators for IOP control to lower normal level and prolonged hypotony in trabeculectomy with mitomycin C were examined to determine conditions required to obtain an optimum balance between them. · Methods: Records of initial trabeculectomy with mitomycin C (0.4 mg/ml, 3 min) in 59 consecutively operated Japanese patients with primary open-angle glaucoma who had used multiple medications before operation were retrospectively analyzed. IOP control to lower normal level was defined as having failed if IOP could not be maintained below 15 mm Hg without medication. Prolonged hypotony was defined as postoperative IOP below 5 mm Hg for more than 2 months, excluding the first month. The Cox proportional harzards model was applied to identify early postoperative factors contributing to the two surgical outcomes. · Results: Among the factors studied, only the mean IOP on postoperative days 9–14 was significantly correlated with the surgical outcome. A receiver operating characteristic plot suggested that mean IOP of 8 mm Hg in this period would give an optimum balance between the two. · Conclusion: IOP of 8 mm Hg 9–14 days after surgery may be advisable in patients with preoperative multiple medications in whom IOP control to lower normal level is attempted with mitomycin C trabeculectomy. Knowledge of this may helpful in deciding when laser lysis of sutures is indicated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004170050100
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