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  • 1
    Electronic Resource
    Electronic Resource
    Analysis of genomic variability in transgenic sugarcane plants produced by Agrobacterium tumefaciens infection (2005)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 124 (2005), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Three transgenic sugarcane populations produced by Agrobacterium tumefaciens infection were analysed for the presence of genomic variability. Plants of the original cultivar, plants regenerated without transformation, as well as transformed and untransformed calli were used as control treatments. Amplified fragment length polymorphism (AFLP) of DNA extracted from leaves or calli assessed genomic profiling. The average DNA polymorphism within each population was determined by calculating the polymorphism index, while the extent of genomic dissimilarity among individual plants within transgenic populations was verified in unweighted pair group method using arithmetic averages dendrograms. The results showed that the production of transgenic sugarcane plants by A. tumefaciens infection is accompanied by limited but detectable genomic changes and that, on average, these occur at the same rate in plant populations carrying different transgenes. Main factors contributing to somaclonal variation in transgenic sugarcane plants have been verified by pre-existing DNA polymorphism into the donor genotype and in vitro culture steps during the transformation procedure. The relevant practical conclusion from this finding is that the AFLP analysis may be effectively used to identify individual transgenic plants with the least genomic deviation from the parental ones. The selected genotype would be conserved as cultivated sugarcane is asexually propagated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.2004.01021.x
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  • 2
    Electronic Resource
    Electronic Resource
    cDNA-AFLP analysis of differential gene expression during the interaction between sugarcane and Puccinia melanocephala (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 123 (2004), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Amplified fragment length polymorphism (AFLP) display of complementary DNA (cDNA) was used to identify genes from sugarcane somaclones expressed during the interaction with Puccinia melanocephala. The isolated transcript-derived fragments (TDFs) correspond to genes involved in the resistance process. Genes related with recognition, signalling and general response were identified through BLAST search. This work constitutes the first report of genes activated during the P. melanocephala-sugarcane interaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.2004.00935.x
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  • 3
    Electronic Resource
    Electronic Resource
    Estudio Histoquímico y Morfométrico de la Población Fibrilar del Músculo Diafragma. Parte I: Caballo y Perro (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Anatomia, histologia, embryologia 24 (1995), S. 0 
    Details
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Histochemical and morphometric study of fibrilar population in diaphragm muscle. Part I: Horse and DogBy using m-ATPase techniques, under alkaline and acid pre-incubations, as well as metabolic techniques (α-MGPDH and NADH-TR), sections from the lumbar and costal portions of diaphragm muscle in horse and dog were analysed. Fibre samples were exposed to image-analysis observations in order to determine the lowest fibre diameter and obtain estadistical data. The findings revealed that the lumbar portion of horse diaphragm consists of fibre types I, IIA and IIB; in the costal portion, the fibre types I and IIA were present. In the dog, the lumbar and costal portions consisted of fibre types I, IIA and IIC, which showed some specific histochemical characteristics.Some data concerning both morphometrical and statistical fibre-type analysis are presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0264.1995.tb00047.x
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  • 4
    Electronic Resource
    Electronic Resource
    Computed Tomography (Ct) of the Liver of the Dog by a Six-generation CT Scanner and Intravenous Contrast Medium (2005)
    Berlin, Germany : Blackwell Verlag GmbH
    Anatomia, histologia, embryologia 34 (2005), S. 0 
    Details
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: By computed tomography the liver has an intermediate degree of attenuation, with a CT value (HU) between 70 and 75. However, this number may vary according to the generation of the apparatus, the use of contrasts medium, the efficiency of the detector, etc. The purpose of the present work was to evaluate the liver of the dog and related structures by means of a six-generation spiral CT scanner (Toshiba Ex Vision [[Au: please provide manufacturers details for ‘Toshiba Ex Vision’: city, state code (if USA) and Country]]), and the previous injection of intravenous contrast medium via cephalic vein. Four half-breed mature dogs (4–6 years, 15–20 kg) were used, two males and two females. The dogs were anaesthetized and kept over a stretcher in sternal recumbency. To evaluate the liver two different CT windows with different window width and window level were applied: soft tissue window (WW 652; WL -34) and mediastinal-vascular window (WW 352; WL 220). The CT images obtained allowed an excellent resolution at the level of the hepatic lobules, the gall bladder, the porta vein, the hepatic artery, the caudal cava vein, etc. The attenuation shades of these structures were very similar in the two used windows, however a better contrast was obtained with the soft tissue window.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0264.2005.00669_118.x
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  • 5
    Electronic Resource
    Electronic Resource
    Magnetic Resonance Imaging of the Normal Canine Larynx (1998)
    Oxford, UK : Blackwell Publishing Ltd
    Anatomia, histologia, embryologia 27 (1998), S. 0 
    Details
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The purpose of this study was to define the normal anatomic structures in the canine larynx with magnetic resonance images. TI-weighted images were taken in the sagittal and transverse planes. The MR images were obtained comparing MR images to dissection planes. Magnetic resonance imaging provides excellent anatomic detail of laryngeal structures. Therefore, it is of value for diagnostic imaging of some respiratory diseases in the dog.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0264.1998.tb00191.x
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  • 6
    Electronic Resource
    Electronic Resource
    Computed Tomography of the Mediastinum of the Dog by a Six-generation CT Scanner, Different Windows and Peroral and Intravenous Contrast Medium (2005)
    Berlin, Germany : Blackwell Verlag GmbH
    Anatomia, histologia, embryologia 34 (2005), S. 0 
    Details
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The anatomical and clinical studies of computed tomography (CT) in the dog are still scarce. Particularly, there are few CT studies combining the use of rapid apparatus of the latest generation, the supply of contrast medium (digestive or intravenous) and the application of regional specific CT windows with different window width (WW) and window level (WL). The use of this CT methodology is particularly recommended in the evaluation of mediastinal masses, heart pathologies, etc. In this work the mediastinum of the dog was studied by using a sixth generation spiral CT scanner (Toshiba Ex Vision), belonging to the private Medical Institute of Radiology ‘Irion’ of Porto Alegre, Brazil. Four mixed-breed mature dogs (4–6 years, 15–20 kg) were used, two males and two females. The dogs were anaesthetized and kept at a maximum inspiration when getting the images. Previously, the urografin® contrast had been injected by peroral and intravenous (cephalic vein) via. Dogs were placed in a stretcher in sternal recumbency. Different CT windows were applied in order to increase the quality of the images and to obtain detailed attenuation shades of the mediastinum organs: soft tissue window (WW 652; WL -34), pulmonary window (WW 928; WL -680) and mediastinal-vascular window (WW 352; WL 220). The mediastinal space was studied craniocaudally from the level of the cranial cava vein, brachiocephalic trunk and left subclavian artery, to the caudal end of the lumbodiaphragmatic recess. The obtained images allowed a very good tomographic evidence of the great vessels, the heart, the thoracic portion of the trachea, the principal bronchi, the thoracic portion of the oesophagus, etc.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0264.2005.00669_23.x
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  • 7
    Electronic Resource
    Electronic Resource
    Congenital Duplication of the Caudal Region (Monocephalus Dipygus) in a Kid Goat (2005)
    Berlin, Germany : Blackwell Verlag GmbH
    Anatomia, histologia, embryologia 34 (2005), S. 0 
    Details
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A case of congenital duplication (monocephalus dipygus) in a goat is described. Two pelvis and four pelvic limbs were observed in the kid. Conjoined or fused symmetric twins were diagnosed. Associated abnormalities were cleft palate and anal atresia. Most of the classically recognized teratogens were ruled out by history and serology. However, progenitors were related in the second degree. Thus, genetic factors could be suspected in this case. To the authors’ best knowledge, this is the first report of monocephalus dipygus in a goat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0264.2004.00570.x
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  • 8
    Electronic Resource
    Electronic Resource
    Anatomy of the Cranioencephalic Structures of the Camel (Camelus dromedarius L.) by Imaging Techniques: A Magnetic Resonance Imaging Study (2005)
    Berlin, Germany : Blackwell Verlag GmbH
    Anatomia, histologia, embryologia 34 (2005), S. 0 
    Details
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The objective of this study was to define the anatomy of the cranioencephalic structures and associated formations in camel using magnetic resonance imaging (MRI). MR images were acquired in sagittal, transverse and oblique dorsal planes, using spin-echo techniques, a magnet of 1.5 T and a standard human body coil. MR images were compared with corresponding frozen cross-sections of the head. Different anatomic structures were identified and labelled at each level. The resulting images provided excellent soft tissue contrast and anatomic detail of the brain and associated structures of the camel head. Annotated MR images from this study are intended to be a reference for clinical imaging studies of the head of the dromedary camel.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0264.2004.00572.x
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  • 9
    Electronic Resource
    Electronic Resource
    Anatomy of the Cranioencephalic Structures of the Goat (Capra hircus L.) by Imaging Techniques: A Computerized Tomographic Study (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Anatomia, histologia, embryologia 26 (1997), S. 0 
    Details
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A topographic study of the cranioencephalic structure was carried out by computerized tomography on Canarian breed adult goats of medium size and weight, with similar cephalic parameters. In this way, transversal, sagittal and horizontal tomographic images were obtained. Identification of the observed anatomic structures represents the basis of this work from which applicable specie data are derived.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0264.1997.tb00119.x
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  • 10
    Electronic Resource
    Electronic Resource
    Estudio Histoquímico y Morfométrico de la Población Fibrilar del Müsculo Diafragma. Parte II: Vaca y Cerdo (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Anatomia, histologia, embryologia 24 (1995), S. 0 
    Details
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Histochemical and Morphometric Study of the Fibrilar Population in Diaphragm Muscle. Part II: Ox and PigM-ATPase techniques, at different pH levels prior to incubation, as well as metabolic procedures (α-MGPDH and NADH-TR), were used to determine the fibre-type content of the lumbar and costal portions in the bovine and porcine diaphragm. The fibre samples were exposed to image analysis in order to evaluate both the morphometrical and statistical values.On the basis of tintorial reactions, the fibre types IA, IB and IIC were found in the lumbar portion of the bovine diaphragm; in the costal portion of the same diaphragm, IA, IB, IIA and IIC fibre types were observed. With regard to the porcine diaphragm, the I, IIA, IIB, IIC fibre types were present in both portions of the muscle. Further morphometrical and statistical data concerning all the fibre types are obtained and discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0264.1995.tb00048.x
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