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  • 1
    Electronic Resource
    Electronic Resource
    Mothproofing wool and wool blends with permethrin (1979)
    s.l. : American Chemical Society
    Journal of agricultural and food chemistry 27 (1979), S. 331-336 
    Details
    ISSN: 1520-5118
    Source: ACS Legacy Archives
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/jf60222a001
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The Na+-Dependent Glutamate and Aspartate Transporter Supports Glutathione Maintenance and Survival of CHO-K1 Cells (1998)
    Springer
    Somatic cell and molecular genetics 24 (1998), S. 341-352 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Glutathione synthesis, a vital cellular process, depends on L-cystine uptake by the amino acid transporter, System x C - . Here we show that a second transporter, System X AG - , is required for normal System x C - activity and glutathione maintenance by employing somatic cell mutants of CHO-K1. Uptake by System x C - in two X AG - -null mutants is significantly lower than that of CHO-Kl, either under control conditions or after prolonged treatment with an electrophile. In addition, levels of glutathione in control and treated mutant cells are less than half those of wild-type CHO-K1 or of a pseudorevertant. The significance of this reduction was tested by chemical challenge: mutants are twofold more sensitive than wild type to reactive oxygen species generated by phenylbenzoquinone and to damage produced by the anticancer drug, cisplatin. These results suggest that System X AG - provides a significant portion of the glutamate used to energize the uptake of cystine required for the synthesis of glutathione.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1024438423899
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Selection of a lysine-resistant CHO-K1 mutant with reduced amino acid transport through multiple systems (1993)
    Springer
    Somatic cell and molecular genetics 19 (1993), S. 331-345 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract High levels ofl-lysine were used to select for resistant variants of Chinese hamster ovary (CHO-K1) cells. Surviving colonies were screened for altered lysine transport and two with reduced uptake were picked. Clone CH-Kr, derived from the more severely affected colony, was analyzed in detail. In starved cells theV max of lysine uptake in CH-Kr was half that of CHO whileK m was unaltered. The intracellular pool of lysine, a substrate of cationic amino acid transport system y+, was significantly lower in CH-Kr. However, transport and pools of other amino acids, which are not substrates of y+, were also reduced in CH-Kr, as was the internal sodium concentration, while hexose import was increased. It appears that the mutation in CH-Kr is pleiotropic, affecting some general aspects of amino acid transport.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01232746
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  • 4
    Electronic Resource
    Electronic Resource
    New mutations and phenotypes associated with glutamate and aspartate transport in Chinese hamster ovary (CHO-K1) cells (1996)
    Springer
    Somatic cell and molecular genetics 22 (1996), S. 87-103 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Two new Chinese hamster ovary cell (CHO-K1) mutants lacking amino acid transport System XAG − activity were isolated by [3H]aspartate suicide selection. These null mutants, Dd-B6 and Dd-B7, were analyzed by somatic cell hybridization, along with previously described partial-function mutants, Ed-A1 and Ed-B8. With respect to System XAG − activity, all four mutations fell into a single complementation group. By quantitative assay, the mutations in Ed-A1 and Ed-B8 behaved as simple recessives in fusions with wild type cells, while those in Dd-B6 and Dd-B7 were codominant. We have discovered that Ed-A1 and Ed-B8 are highly permeable to smal neutral molecules. This high permeability phenotype was dominant to wild-type. Northern, Southern, and Western analyses indicated that System XAG − in CHO is not closely related to any of the three well characterized glutamate transporters represented by GLT-1, EAAC1 or GLAST.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02369900
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  • 5
    Electronic Resource
    Electronic Resource
    Selection of Chinese hamster ovary cells (CHO-K1) with reduced glutamate and aspartate uptake (1993)
    Springer
    Somatic cell and molecular genetics 19 (1993), S. 231-243 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Transport of L-[3H]glutamate into Chinese hamster ovary cells (CHO-K1) was characterized and the results used to design a tritium suicide selection for cells with transport defects. Replicas of surviving colonies on polyester cloth disks were scrrened by autofluorography for reduced uptake and two mutant clones, Ed-A1 and Ed-B8, were obtained. Uptake of glutamate through a sodium-dependent system in both mutants was characterized by significant reductions inV max and increases inK m compared to parental cells, but their response to removal of extracellular sodium differed, suggesting distinct mutations in the two lines. TheV max of aspartate uptake through this system was reduced in both mutants, to one-ninth in the case of Ed-B8. Glutamate uptake through a sodium-independent system was not altered in either mutant. Surprisingly, acid-soluble intracellular pools of several amino acids were higher in both mutants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01233071
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    Paper (German National Licenses)
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