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Electrophoretic Analysis of Endonuclease-Generated Fragments of k-DNA, of Esterase Isoenzymes, and of Surface Proteins as Aids for Species Identification of Insect Trypanosomatids (1982)

CAMARGO, E. PLESSMANN ; MATTEI, D. M. ; BARBIERI, CLARA L. ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

The @journal of eukaryotic microbiology 29 (1982), S. 0

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ISSN: 1550-7408

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: In order to verify the applicability of biochemical methods for species identification of Trypanosomatidae, 13 species of monoxenic trypanosomatids plus the heteroxenous Trypanosoma cruzi were comparatively analyzed by three different biochemical methods. Insect trypanosomatids examined were: Crithidia acanthocephali, C. fasciculata (three varieties), C. luciliae luciliae, C. luciliae thermophila, C. deanei, C. oncopelti, Herpetomonas muscarum muscarum, H. megaseliae, H. samuelpessoai, H. mariadeanei, Leptomonas seymouri, L. collosoma, L. samueli, and Blastocrithidia culicis. Also included in the survey were aposymbiotic strains of C. deanei and C. oncopelti. Methods used were: electrophoretic profiling of endonuclease-generated fragments of k-DNA, esterase isoenzymes profiling, and polyacrylamide-gel electrophoresis (SDS-PAGE) of radioiodinated cell surface proteins. Interspecific but not intraspecific differences were detected by all three methods among the 13 monoxenic species examined. Thus, it is concluded that these methods can be successfully used, in addition to classical criteria, for species identification of insect trypanosomatids.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1550-7408.1982.tb04022.x

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Depletion of secondary lysosomes in mouse macrophages infected withLeishmania mexicana amazonensis: A cytochemical study (1985)

Barbieri, Clara L. ; Brown, Kristy ; Rabinovitch, Michel

Springer

Parasitology research 71 (1985), S. 159-168

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ISSN: 1432-1955

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Abstract Leishmania amastigotes lodge and multiply within parasitophorous vacuoles, which can fuse with secondary lysosomes of the host macrophages. This study examines the effect of infection with amastigotes ofL. mexicana amazonensis on the secondary lysosomes of mouse macrophage cultures. The cultures were stained for the activities of two lysosomal enzyme markers, acid phosphatase and arylsulfatase, and the light microscopic observations were supplemented by electron microscopy. Nearly all noninfected macrophages contained numerous stained secondary lysosomes. The number of such lysosomes was markedly reduced 24 h postinfection, and the reduction persisted for at least 10 days. Stained secondary lysosomes reppeared after the amastigotes were destroyed by exposure of the cultures to phenazine methosulfate or by placing them at 37.5° C. The depletion of lysosomes shown by cytochemical methods may reflect a high rate of fusion of the lysosomes with the parasitophorous vacuoles, exceeding the rate of formation of new secondary lysosomes. Alternatively, the parasites may inhibit the synthesis of lysosomal hydrolases, or the assembly or formation of primary or secondary lysosomes.