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  • 1
    Electronic Resource
    Electronic Resource
    Immunoglobulin E is not required for but enhances airway inflammation and hyperresponsiveness (2003)
    Oxford, UK : Munksgaard International Publishers
    Allergy 58 (2003), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The aim of this study was to investigate the role of immunoglobulin E (IgE) in the late phase reaction (LPR) of murine experimental asthma. Our model consisted of an implant of DNP-conjugated, heat-coagulated hen's egg white (DNP-EWI), followed 14 days later by an intratracheal challenge with aggregated DNP-ovalbumin. Airway inflammation was analyzed 48 h after challenge and compared with a similarly immunized group of mice with highly suppressed humoral response due to anti-μ and anti-δ antibody treatment. Total number of cells in the bronchoalveolar lavage (BAL) (with predominance of eosinophils) and EPO activity in the lung homogenate were increased in the DNP-EWI-immunized group compared with immunosuppressed or nonimmunized mice. However, the cellular infiltration and EPO activity observed in the immunosuppressed group were still significantly above those obtained in the nonimmunized group, indicating that inhibition of antibody production did not completely prevent the inflammatory manifestations in BAL and lung. Airway hyperresponsiveness to methacoline was obtained in DNP-EWI-immunized mice, but the respiratory mechanical parameters returned to normal levels in the immunosuppressed group. When these mice were reconstituted with monoclonal anti-DNP antibodies, only IgE, but not IgG1, restored lung inflammation and decreased the conductance of the respiratory system, therefore, increasing hyperresponsiveness. These results indicate that antibodies are not essential for induction of LPR in the lung. However, IgE enhances pulmonary inflammation and hyperresponsiveness.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1398-9995.2003.00206.x
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  • 2
    Electronic Resource
    Electronic Resource
    The Involvement of Kupffer Cells in the Clearance of High Molecular Weight Rat IgA Aggregates in Rats (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 31 (1990), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In the present study the clearance kinetics and tissue distribution of aggregated 125I-labclled monoclonal rat IgA ([125I] AIgA) of different sizes were studied in rats. Soluble [125I]AIgA disappeared from the circulation in a biphasic manner with an initial rapid distribution half-life (TI) and a second slower half-life (T2). T2 was directly related to the size of the aggregates. High molecular weight [125I]AIgA, containing 10–12 IgA molecules per aggregate ([lgA]10–12). was cleared much faster than low molecular weight aggregates. The main site of clearance was the liver. The larger the size of the AIgA, the more degradation products were found in the circulation. After injection of [[lgA]10–12, non-parenchymal cells (NPC) contained three times more radioactivity than parenchymal cells(PC)(NPC:P ratio 3.06 ± 0.96). Ratios of 0.82 ± 0.0.3 and 0.62 ± 0.12 were observed when [IgA]5–6 and [IgA]2 were injected respectively, suggesting a greater role for Kupffer cells in the clearance of large-sized IgA aggregates. Kupffer cells were shown to be the main cells for localization of large-sized AIgA established by immunohistochemical staining on liver cryostat sections.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1990.tb02819.x
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  • 3
    Electronic Resource
    Electronic Resource
    Rat C3 Conversion by Rat Anti-2,4, Dinitrophenyl (DNP) Hapten IgA Immune Precipitates (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 25 (1987), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Moncmeric (m-) and polymeric (p-) anti-DNP monoclonal (MC) rat IgA antibodies (Ab) were tested for precipitation with DNP-bovine serum albumin (DNP-BSA) and C3 conversion in rat serum, with rat MC anti-DNP igG2b used as reference. At equivalence, p-IgA rapidly precipitated DNP-BSA, with little antigen (Ag) left in the supernatant. In contrast, m-IgA at five-fold higher concentration precipitated Ag very slowly, with 〈50% of Ag precipitated at equivalence. The Ag/Ab weight ratio at equivalence was 0.13 for both m- and p-IgA, but the molar ratio was 0.3 for m-IgA and close to 1.0 for p-IgA, suggesting a higher avidity of p-IgA. Rat C3 conversion by rat IgA immune precipitates (IP) was about 20% with m-IgA and 40% with p-IgA. EGTA did not significantly affect these figures. Therefore, rat MC IgA IP activated the rat alternative C pathway. Neither rat nor mouse IgA anti-DNP IP activated C3 in normal human serum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1987.tb02201.x
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  • 4
    Electronic Resource
    Electronic Resource
    ANTIBODIES INDUCED BY LOCAL ANTIGENIC STIMULATION OF MUCOSAL SURFACES (1971)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 190 (1971), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1971.tb13540.x
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  • 5
    Electronic Resource
    Electronic Resource
    GENETIC REGULATION OF IMMUNOGLOBULIN SYNTHESIS: SELECTION OF TWO RATS' LINES WITH RESPECTIVELY A HIGH OR LOW IgM SERUM LEVEL1 (1983)
    Oxford, UK : Blackwell Publishing Ltd
    International journal of immunogenetics 10 (1983), S. 0 
    Details
    ISSN: 1744-313X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Two directional selections of rats for a high or a low IgM serum levels have been carried out. The criterium of selection was the individual merit of each rat in having a high or low IgM level when they were 3 months old. All animals were kept together, in order to avoid external influence on the IgM synthesis. The founding population was a mixed nucleus of rats, obtained by breeding of 13 different strains of inbred or outbred rats for two generations. Two lines, one with a high IgM serum level and another with a low IgM serum level, have been separated. At generation 10, they differed from each other by a coefficient of approximately four. These IgM serum levels were not due to differences in IgM catabolism of the two lines since both lines had similar IgM half-lives. Analyses of the data obtained show that, for IgM synthesis, the coefficient of heritability varies between 0.30 to 0.40, and the number of `independent loci' controlling IgM synthesis ranges from 11 to 14.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1744-313X.1983.tb00806.x
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  • 6
    Electronic Resource
    Electronic Resource
    The Non-Specific Enhancement of Allergy (1983)
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 38 (1983), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: PVG tats given injections of 1 μg ovalbumin (OA) together with 10 mg Silica gel failed lo provide serosal mast cells or lung tissue with the capacity to release histamine on in vitro challenge with the antigen. However, if such animals were injected i.p. with 100 mg of alum (without any further antigen addition) 3–9 weeks after the primary antigen injection, their mast cells and lung tissue showed a clear-cut capacity to respond in vitro, when examined 1 week after the alum injection. Injection of only 15 mg alum did not induce such a response capacity. The lading of the reactivity induced by an alum injection could be prevented by a repeated injection of the adjuvant alone. Pretreatment of the rats with cyclophosphamide (33 mg/kg) 2 days before the primary antigen injection did not affect the response capacity induced by a booster injection 3 weeks later. S.c. injection of alum also precipitated response capacity in animals primed by i.p. injection of antigen and Silica gel. The anaphylactic response capacity induced by injection(s) of alum was generally accompanied by increased levels of OA-IgE and especially OA-IgG2a antibody; however, a clear-cut correlation between either serosal mast cell or lung tissue response capacity and serum OA-IgE or IgG2a antibody titer could not be demonstrated. These data show that in primed animals, which do not express allergic response capacity, such a capacity can be induced by injecting adjuvant alone, even several weeks after the primary antigen injection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1398-9995.1983.tb01618.x
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  • 7
    Electronic Resource
    Electronic Resource
    Lymphocyte plasma membranes. III. Composition of lymphocyte plasma membranes from normal and immunized rats
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Biomembranes 382 (1975), S. 506-525 
    Details
    ISSN: 0005-2736
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0005-2736(75)90218-7
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  • 8
    Electronic Resource
    Electronic Resource
    The use of Xenopus egg cells to assay the mRNA of single cells (1978)
    [s.l.] : Nature Publishing Group
    Nature 271 (1978), S. 479-481 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Fig. 1 In ovo synthesis of rat IgE. Female LOU/M/WS1 rats were infected with 0.5 ml myeloma IR2 cells (IgE-secreting myeloma10). After 7-9 d ascites was tapped and on average it contained 12.6 x 106 cells per animal. These were diluted 1:1 with Earle's balanced salt solution and layered on top of ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/271479a0
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  • 9
    Electronic Resource
    Electronic Resource
    Concentration of Immunoglobulins in Lethally X-irradiated Mice (1967)
    [s.l.] : Nature Publishing Group
    Nature 215 (1967), S. 742-744 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Twenty male CBA/H mice, 5 months of age, were used. Seven received 1,000 rads (250 keV, 14 m.amp., half value thickness 1-2 mm copper), seven received 800 rads, and the remaining six served as non-irradiated controls. Irradiation was administered ventro-dorsally to the whole body. The twenty mice ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/215742a0
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  • 10
    Electronic Resource
    Electronic Resource
    ^3^2P Labeling of Nonnucleosidic Moieties 5'-Attached to Oligonucleotides
    Amsterdam : Elsevier
    Analytical Biochemistry 214 (1993), S. 338-340 
    Details
    ISSN: 0003-2697
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1006/abio.1993.1501
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