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  • 1
    Electronic Resource
    Electronic Resource
    Mites and house dust allergy. IV. Antigen and allergen(s) of Dermatophagoides pteronyssinus extract (1975)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 5 (1975), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The antigenic composition of D. pteronyssinus extract was investigated by using antisera raised in rabbits by injection of the mile extract in Freund's adjuvant. Immunodiffusion analysis revealed the presence in the mite extract of many antigenic constituents, most of which were also common to human dander and house dust extracts. Four antigenic components not shared by human dander appeared to be common to D.farinae extract. D. pteronyssinus extract was then fractionated on Sephadex G-75 and DEAE-Sephadex. Immunodiffusion analysis of the most active fraction revealed, after heat-treatment, the presence of three precipitin lines, one of which appeared not to be shared by nutrient medium or by D. farinae extract constituents.When the anti-mite rabbit serum was fixed to an immunosorbent and incubated with a house dust extract it was constantly able to induce a strong decrease or a complete loss of the allergenicity of the dust extract in man. These results suggest that mite allergen(s) are immunogenic in the rabbit and provide more direct evidence for identity of the allergens in house dust and mite.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.1975.tb01837.x
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  • 2
    Electronic Resource
    Electronic Resource
    Mites and house dust allergy (1972)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 2 (1972), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: House dust and mite (Dermatophagoides pteronyssinus and D. farinae) dialysed extracts were fractionated by gel filtration and ion-exchange chromatography. The protein and sugar content of each fraction was determined, respectively, by absorbance at 280 mμ and by the orcinol method. Pooled fractions were injected into house dust and mite sensitive patients and their capacity to induce positive skin reactions determined. A certain amount of allergenically active material was dialysable but the greater part remained inside the dialysis bag. The most active non-dialysable house dust and mite extract material was eluted in the excluded Sephadex G-50 fractions and appeared to be located immediately after the Sephadex G-75 excluded fractions. On the basis of calibration curves, its molecular weight was estimated between 10,000 and 50,000.By further chromatography of active Sephadex fractions on DEAE-Sephadex, the most potent antigen-containing material of house dust and mite extracts was recovered using 0-3 and 02 M NaC1 as elution media. These data offer further evidence of the existence of a close relationship between the major antigenic substances of house dust and mite extracts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.1972.tb01276.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Mites and house dust allergy III. In vitro lymphocyte transformation and precipitating antibody to house dust and mite (Dermatophagoides pteronyssinus) extract in atopic and non-atopic individuals (1973)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 3 (1973), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In vitro lymphocyte transformation and the precipitating antibody to house dust and mite (Dermatophagoides pteronyssinus) extract were studied in mite- and house-dust sensitive patients and in non-atopic individuals. House-dust extract was able to induce a significant [2-14C]thymidine incorporation into lymphocytes from house-dust-sensitive as well as non-allergic individuals. An in vitro response to mite extract, even if less marked, was also seen in most of mite-sensitive patients.By the micro-Ouchterlony and counterimmunoelectrophoresis techniques anti-dust antibodies were found in the serum of all house-dust-sensitive and non-atopic individuals tested. Anti-mite precipitins were detected in a smaller number of subjects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.1973.tb01309.x
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  • 4
    Electronic Resource
    Electronic Resource
    Mites and house dust allergy (1972)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 2 (1972), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: 279 patients, most of whom had perennial asthma or perennial rhinitis, were tested intradermally with extracts of Dermatophagoides pteronyssinus and D. farinae in order to ascertain the incidence of positive skin reactions. The great majority of house dust sensitive patients had positive skin reactions to both mite extracts. However, the concordance between house dust and mite extract positive skin reactions, though statistically significant, did not reach the extent found in other countries.Of the three extracts employed, D. pteronyssinus appeared to contain the most potent allergen while its culture medium extract yielded a low percentage of positive skin reactions. These results suggest that, in italy also, mites seem to represent the most important allergenic source of house dust.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.1972.tb01275.x
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  • 5
    Electronic Resource
    Electronic Resource
    VIP-, substance P- and enkephalin-immunoreactive innervation of the human gastrointestinal mucosa
    Amsterdam : Elsevier
    Regulatory Peptides 7 (1983), S. 285 
    Details
    ISSN: 0167-0115
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0167-0115(83)90051-4
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  • 6
    Electronic Resource
    Electronic Resource
    Peptide-containing innervation of the human intestinal mucosa (1982)
    Springer
    Histochemistry and cell biology 76 (1982), S. 413-420 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The three-dimensional distribution of the peptide-containing invervation in the human intestinal mucosa was studied by fluorescence immunohistochemistry on whole-mount mucosal preparations. An extensive VIP-immunoreactive nerve supply was demonstrated at all levels, but was markedly increased in density in the distal intestine, where it formed a particularly rich network in close contact with the luminal epithelium. In contrast, substance P-containing nerve fibres formed a looser and evenly distributed innervation at all levels. The muscularis mucosae was richly supplied by VIP-and substance P-containing fibres. Met-enkephalin immunoreactivity was confined to a few scattered nerve bundles running in the muscularis mucosae and around the bottom of epithelial crypts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00543961
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