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  • 1
    Electronic Resource
    Electronic Resource
    Biochemical Specification versus Specific Regrowth in the Innervation of Skin Grafts in Anurans (1972)
    [s.l.] : Nature Publishing Group
    Nature 236 (1972), S. 235-237 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The experiments reported here were designed to test the alternative hypothesis of specific regrowth of nerves to the sites at which they originally terminated7. There are three ways in which this might occur. (1) Nerves cut during the transplantation operation regenerate towards the periphery but, ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/236235a0
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Selective inhibition of the Na+/H+ exchanger type 3 activates CO2/H+-sensitive medullary neurones (1999)
    Springer
    Pflügers Archiv 438 (1999), S. 255-262 
    Details
    ISSN: 1432-2013
    Keywords: Key words Chemosensitivity ; Intracellular pH ; Na+/H+ exchanger ; Organotypic medullary culture ; Respiration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Hypercapnia as well as lowered intracellular pH (pHi) increase the bioelectric activity of CO2/H+-sensitive neurones (VLNcs) of the ventrolateral medulla oblongata. Here we describe that immunoreactive Na+/H+ exchanger (NHE3) is present in ventrolateral neurones from medullary organotypic cultures (obex level). To test whether VLNcs can be acidified and thereby activated by inhibition of NHE3, we used the novel high-affinity NHE3-inhibitors S1611 and S3226. Both drugs raised the firing rates of VLNcs to at least 150% of the control values, and depolarized membrane potential by up to 15 mV at concentrations (0.5–1 µmol/l) suitable for selective inhibition of NHE3. The changes in bioelectric activity strongly resembled the responses to hypercapnia (PCO2: 60–100 mmHg). In BCECF-AM-loaded cultures a subfraction of ventrolateral VLNcs was found to be intracellularly acidified by 0.05–0.1 pH units following treatment with S1611; the time course of this acidification was similar to that evoked by hypercapnia. All drug effects were sustained and readily reversible upon washing. Non-CO2/H+-responsive medullary neurones as well as hippocampal CA3 neurones were unaffected by up to 20 µmol/l S1611. It is concluded that the selective inhibition of NHE3 acidifies and activates CO2/H+-sensitive neurones within the ventrolateral medulla oblongata.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004240050907
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  • 3
    Electronic Resource
    Electronic Resource
    Central neuronal responsiveness to sensory ganglion stimulation is correlated with the incidence of spontaneous bioelectric activity in developing spinal cord cultures (1987)
    Springer
    Pflügers Archiv 410 (1987), S. 563-565 
    Details
    ISSN: 1432-2013
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In spinal cord explants co-cultured with dorsal root ganglion cells for 3–4 weeks in a (horse)serum-containing medium, the spread of ganglion-evoked action potentials from monosynaptic innervation sites (“polysynaptic excitability index”) was not correlated with the incidence of neuronal “background” discharges. Moreover, chronic exposure of serum-grown cultures to tetrodotoxin (TTX) in a dose sufficient to reversibly block bioelectric activity, failed to significantly affect this index. For explants grown in a chemically defined medium (CDM) similar excitability scores were obtained only if a low level of spontaneous activity was measured. The most active preparations scored considerably higher, with intermediate values being found in the moderately active cultures. Chronic TTX-exposure in developing CDM-grown cultures reduced their excitability scores to the level found in weakly active, untreated, explants despite a normal incidence of spontaneous activity. The present study indicates that low levels of spontaneous activity in untreated explants were associated with a similar sluggishness of DRG-evoked responses as previously observed after chronic treatment with TTX. These results give additional grounds for confidence that this reduced responsiveness of spinal cord neurons to sensory input is indeed attributable to prolonged reduction of centrally generated excitation during development in vitro.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00586541
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    Paper (German National Licenses)
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