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  • 1
    ISSN: 1432-072X
    Keywords: Key words Carboxysome ; Thiobacilli ; RuBisCO ; CsoS2 ; Glycosylation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The carboxysomal polypeptides of Thiobacillus neapolitanus with apparent molecular masses of 85 and 130 kDa were isolated and subjected to N-terminal sequencing. The first 17 amino acids of the two peptides were identical. The sequence perfectly matched the deduced amino acid sequence of an open reading frame in the carboxysome operon. The gene was subsequently named csoS2. Expression of the gene in Escherichia coli resulted in the production of two peptides with apparent molecular masses of 85 and 130 kDa. Immunospecific antibodies generated against the smaller peptide recognized both peptides; the peptides were named CsoS2A and CsoS2B, respectively. A digoxigenin-hydrazide glycosylation assay revealed that both CsoS2A and CsoS2B are post-translationally modified by glycosylation. CsoS2 was localized to the edges of purified carboxysomes by immunogold electron microscopy using the monospecific CsoS2A antibodies. The molecular mass of CsoS2A calculated from the nucleotide sequence was 92.3 kDa.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 71 (2000), S. 3178-3183 
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: The design and operation of a gas aggregation source is described. The source combines the attributes of high-temperature operation (enabling preparation of transition metal clusters), mass selection, ultrahigh vacuum compatibility, and transportability. This makes it ideally suited to in situ studies such as scanning tunneling microscope or synchrotron radiation experiments. Data are presented to illustrate the performance of the source; recent results obtained in synchrotron radiation studies are highlighted. © 2000 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 68 (1997), S. 1853-1857 
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: The design and operation of a high temperature gas aggregation source, capable of in situ deposition of mass-selected atomic clusters of transition metals onto a substrate in an ultrahigh vacuum chamber, is described. Mass-selection is achieved by an ultrahigh mass quadrupole filter operating at masses up to 3×104 amu. © 1997 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 88 (2000), S. 3414-3417 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The magnetic properties of Fe nanostructured films have been studied using magnetic linear and circular dichroism in x-ray photoemission spectroscopy. The samples were prepared by the deposition of nanoscale Fe clusters, size 1–4 nm, onto thin Cu films. The linear dichroism, which is used to measure the in-plane magnetization, increases with increasing film thickness, with a sharp increase between 1 and 1.5 ML coverage. The circular dichroism, which measures the out-of-plane magnetization, is zero within the experimental error at all thicknesses studied. Capping an Fe film with an ultrathin Pd layer results in a factor of 3 decrease of the linear dichroism response. © 2000 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 86 (1999), S. 2651-2654 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Fe particles with sizes in the range 1–5 nm, formed by a gas-aggregation method and deposited onto graphite and C60 supports, were studied by x-ray photoemission spectroscopy, x-ray absorption spectroscopy, and magnetic linear dichroism. Clusters deposited onto a C60 coated graphite substrate become embedded within the fullerene film, and have an increased resistance to oxidation compared to exposed clusters supported on a graphite surface. No evidence for hybridization between the electronic states of Fe and C60 is seen. The magnetic dichroism signal of the exposed clusters increases sharply with the film thickness because of the increased cluster interactions. © 1999 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-0832
    Keywords: Agriculture ; Biocontrol ; Residue ; Saprophytes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Thirteen species of fleshy fungi were identified from two agricultural fields during the 1989 and 1990 growing seasons at the Rural Development Center (RDC) and Horticulture Farm, Coastal Plain Experiment Station, Tifton, Georgia. The three most common fungi wereCoprinus plicatilis, Cyathus olla, andPanaeolina foenisecii. At the former site, residue management practices were established for evaluating crop production and at the Horticulture Farm different crops were grown with conservation tillage employing a Low Input Sustainable Vegetable Production system. Tillage treatments at the RDC were no-till, row-till, ridge-plant, and moldboard-plowing. Residue management of triticale included burning or cutting the stubble at a height of 20 or 60 cm at harvest. At the Horticulture Farm, seven winter cover crops were compared to a fallow control. No significant differences were shown between observations of fungal species and individual treatments at the Horticulture Farm during 1989 and 1990. However, there were treatment differences for observed fungi at the RDC farm on specific dates for both years. Furthermore, no individual treatment(s) during the investigation increased observation rates of specific fungal species. Non-burned debris treatments showed consistently higher populations and diversity of fungi than burn debris plots.
    Type of Medium: Electronic Resource
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