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  • 1
    Electronic Resource
    Electronic Resource
    Milk lipid absorption and chylomicron production in the suckling rat (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 195 (1979), S. 397-412 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Structural correlates of milk lipid absorption and chylomicron production were studied in 10-day-old suckled rats. The gastric and duodenal contents and duodenal mucosae were examined with the light and electron microscopes. In the gastric lumen the milk lipid globule cores were smooth, circular and uniformly electron opaque. Many membranes and lamellar structures with a trilaminar and multilamellar appearance were adherent to the peripheries of the cores. In the central duodenal lumen the milk lipid globule cores were also smooth, circular and uniformly electron opaque. Very few milk lipid globules in the duodenal lumen showed adherent membranes or lamellae. Membrane fragments and lamellae were present in the lumen separate from the milk lipid globules. In the duodenal lumen between villi the milk lipid globules had multiple electron lucent indentations of the core. It is believed that the irregular peripheries of the milk lipid globule cores are the result of lipolysis within the duodenal lumen acting at the milk lipid globule surface. This lipolysis of triacylglycerol would produce amphiphilic lipids which may result in the electron lucent spaces at the milk lipid globule periphery. The absorptive epithelial cells along the length of the duodenal villus varied in structure relative to their position at the tip, middle, or base of the villus. Typical mid-villus epithelial cells contained lipid droplets averaging 0.3-μm diameter in the smooth and rough endoplasmic reticulum and in Golgi complexes in the apical cytoplasm. Villus tip and villus base cells contained large lipid droplets between 7-16 μm. Only a few 0.3-μm lipid droplets were present within these cells. These large lipid droplets appeared to be accumulations of triacylglycerol present in the apical cytoplasm associated with lamellar and membranous structures. Numerous chylomicrons were present between epithelial cells located in the middle region of the villus while significantly fewer chylomicrons were seen between epithelial cells at the tip and base of the villus. These observations suggest that the cells at the middle of the duodenal villus of suckling rats were more efficient in the production of chylomicron triacylglycerol derived from incoming milk triacylglycerol than cells at the tip and base of the villus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091950301
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  • 2
    Electronic Resource
    Electronic Resource
    An ultrastructural morphometric comparison of the right and left sides and lobes of the dog lung (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 204 (1982), S. 39-44 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Regional variations of the components of lobes of the lung have been recognized to result from gravitational effects. Excised and isolated perfused lungs are frequently used as objects for studies of fluid flow. This ultrastructural study considered differences between the right and left sides and lobes of the excised lung. The lungs of three mongrel dogs were fixed by simultaneous endotracheal instillation of glutaraldehyde and immersion in fixative. Stratified random sampling, point-counting volumetry, and line intercept counts were utilized to determine the volume densities of alveolar space, endothelial vesicles, interstitial cells, fibers, and matrix. Thicknesses of the air-blood barrier, epithelial, interstitial, and endothelial compartments were measured. Values for the right side were compared to values for the left by t-test. Values for lobes were compared statistically by a two-way analysis of variance. There was no statistically significant column effect, no row effect, and no interaction between the mean measurements of the factors computed for the right and left lungs and the lobes within the lungs. It is concluded that stratified random sampling combined with endotracheal and immersion fixation as used in this study equalize regional differences in the alveolar lumen volume and air-blood barrier thickness. With these conditions there are no significant differences by sides or lobes in the volume densities of the epithelial and endothelial cells and components of the interstitium of the air-blood barrier.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092040106
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  • 3
    Electronic Resource
    Electronic Resource
    A light and electron microscopic study of Kurloff bodies in the blood and spleen of the guinea pig (1966)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 156 (1966), S. 107-117 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The Kurloff body of guinea pig mononuclear leukocytes was studied by light and electron microscopy of blood and spleen. Electron microscopy disclosed the presence of extensively dilatedendoplasmic reticulum, clusters of small vesicles, and clumps of long parallel fibers in the cytoplasm. The osmiophilic inclusions contained many myelin figures, both around the periphery and free in the matrix. Evidence for motility of these cells was noted.Rods, filaments, and spheres seen in supravital preparations appear as myelin figures when seen with the electron microscope. Although historically considered, parasitism, nuclear extrusion and phagocytosis of cells are unlikely as causal in the formation of the inclusion. Being based on contradictable evidence, a lysosomal origin is doubted. The formative process isfelt to be either one of intracellular secretion or of a sequestering and concentration of a serum molecular component.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091560113
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  • 4
    Electronic Resource
    Electronic Resource
    Morphometry of tubular bodies in endothelial cells in normal, stable isolated perfused, and edematous dog lungs (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 196 (1980), S. 295-300 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The volume densities and dimensions of tubular bodies (Weibel-Palade bodies) in dog pulmonary capillary endothelial cells were examined by means of stratified random sampling and electron microscopic morphometry. The mean volume density of tubular bodies in normal (control) lungs was 0.43 ± 0.29%. The mean volume densities of tubular bodies in stable, isolated lungs perfused for 1/2 hour, 1 hour, and 2 hours and in lungs made edematous by increasing hydrostatic pressure or by decreasing oncotic pressure did not vary significantly from that of normal lungs. The mean thickness of the endothelium measured at the middle of the tubular bodies of normal dog lungs was nearly twice the mean thickness of the overall capillary endothelial cell sample. The mean endothelial thickness across tubular bodies from stable and from edematous isolated perfused lungs did not differ significantly from that of the control group. The mean width of tubular bodies from normal dog lungs was 0.25 ± 0.06 μm and the mean length was 0.81 ± 0.61 μm. The mean widths and lengths of tubular bodies from stable and from edematous isolated perfused dog lung endothelial cells did not differ statistically from those of normal dog lungs. Thirty percent of the tubular bodies in the sample were found to be adjacent to a mitochondrion in the same plane of section. Tubular bodies contained both tightly packed and loosely grouped tubules. It is concluded that the tubular bodies in canine pulmonary endothelial cells remain stable during the perfusion of isolated lungs and in oncotic and hydrostatic edema of isolated perfused lungs.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091960305
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  • 5
    Electronic Resource
    Electronic Resource
    An ultrastructural morphometric comparison of the peripheral with the hilar air-blood barrier of dog lung (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 209 (1984), S. 535-540 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Excised and isolated perfused lungs are frequently used as models for studies of fluid flow and membrane transport.Regional variations of the air and blood vascular compartments of the lung have been recognized to result from gravitational effects. This ultrastructural morphometric study considered the components of the air-blood barriers of the peripheral and hilar regions of excised dog lungs. The lungs of three mongrel dogs were fixed by endotracheal instillation of glutaraldehyde and immersion in fixative. Stratified random sampling, point counting volumetry, and line intercept counts were used to determine the thicknesses of the air-blood barrier and the epithelial, interstitial, and endothelial compartments. Point counting volumetry also established the volume density of the alveolar spaces. The morphometric values for structures within the periphery were statistically compared to those within the hilar region by Student's t-test. Endotracheal and immersion fixation as used in this study, combined with stratified random sampling, equalized regional differences in the alveolar lumen volume and airblood barrier thickness. With these conditions no significant differences in the volume densities of the epithelial and endothelial cells and the interstitium of the air-blood barrier were identified when those of the hilum were compared to those of the periphery.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092090413
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  • 6
    Electronic Resource
    Electronic Resource
    Sites of lipoprotein production in the small intestine of the unsuckled and suckled newborn ratThis investigation was supported by a grant from the General Research Fund of the College of Medicine and Dentistry of New Jersey (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 195 (1979), S. 15-29 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The size and distribution of lipoprotein particles in the intestinal epithelium were determined before and after the start of suckling by infant rats. Prior to suckling very low density lipoprotein particles were within the smooth endoplasmic reticulum (SER), rough endoplasmic reticulum (RER), Golgi apparatus and interepithelial cell spaces of duodenal villus tip cells. Chylomicrons were produced in the SER, RER and Golgi apparatus of duodenal epithelial cells after the commencement of suckling.Ileal epithelial cells did not contain particles of VLDL dimensions prior to suckling. Ileal cells of rats suckled for 24 hours after birth had VLDL particles in the SER, RER and Golgi apparatus above and at the sides of the large supranuclear vacuole in each cell. Ileal cells at 10 and 15 days of age had fewer and smaller particles. These particles were also present in the interepithelial cell spaces and lacteals.It is suggested that VLDL particles are produced by duodenal cells prior to suckling when only endogenous lipid is available. The ingestion of exogenous lipids in milk is associated with the production of chylomicrons by duodenal epithelial cells and VLDL particles by ileal epithelial cells. VLDL particles within ileal cells become smaller and less numerous as suckling continues, possibly due to increased pancreatic lipase. This is interpreted as evidence that chylomicrons and intestinal VLDL particles are related.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091950103
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  • 7
    Electronic Resource
    Electronic Resource
    Phagocytic inability of Kurloff cells in the lung and spleen of the guinea pig (1979)
    Springer
    Cell & tissue research 204 (1979), S. 513-517 
    Details
    ISSN: 1432-0878
    Keywords: Kurloff cells ; Lung ; Spleen ; Phagocytosis ; Latex spheres
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Adult female guinea pigs received subcutaneous implants of diethylstilbestrol-cholestrol pellets which produced splenomegaly and increased numbers of splenic Kurloff cells. Latex spheres subsequently injected intravenously were not phagocytized by Kurloff cells within the lungs and spleen as examined with the electron microscope. This is considered as evidence that Kurloff cells are probably not phagocytic. The origin of these cells is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233661
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  • 8
    Electronic Resource
    Electronic Resource
    Isolated perfused dog lungs: A comparative stereologic comparison with normal dog lungsThis investigation was supported in part by Public Health Service Grants HL-19571 and HL-12879. (1978)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 151 (1978), S. 337-344 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Established stereologic techniques were used to evaluate the morphologic integrity of isolated dog lungs perfused with plasma for periods of 30 minutes, 1 hour and 2 hours. The morphometric data from the isolated lung preparations were compared to similar morphometric evaluations of dog lungs fixed immediately after removal from the thorax. In the isolated lungs capillary surface and volume densities were both substantially decreased. These estimations of capillary surface density provide a morphologic definition of capillary surface area which should be useful in the estimations of endothelial permeability in isolated lungs. This morphometrically defined decrease in capillary volume density was attributed, in part, to swelling of the endothelial cells. Alveolar surface density was also decreased and the type-I epithelial cells were increased in thickness. In both the endothelial and epithelial cells, cytoplasmic volume densities of the mitochondria and smooth endoplasmic reticulum were increased, while those of the rough endoplasmic reticulum and micropinocytotic vesicles were unchanged. The thickness of the interstitial compartment of the air-blood barrier and the volume densities of the peribronchial, peribronchiolar, and perivascular connective tissue sleeves were unchanged; there was no evidence of interstitial edema in the isolated lungs. These morphologic changes must be considered in the interpretation of physiologic studies which employ isolated perfused dog lung preparations.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001510303
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  • 9
    Electronic Resource
    Electronic Resource
    A morphometric examination of type II alveolar epithelial cells in normal and isolated-perfused dog lungsThis investigation was supported in part by Public Health Service Grants HL-19571 and HL-12879. (1977)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 150 (1977), S. 139-147 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The volume densities of type II alveolar cell cytoplasmic organelles and alveolar surface densities were estimated by established stereologic procedures. The morphometric measurements were obtained from normal dog lungs (in situ) and isolated dog lungs perfused for 30-minute, 1-hour, and 2-hour periods. The type II cell lamellar body volume densities and the alveolar surface densities progressively decreased as the times of perfusion were increased. The volume densities of the granular and agranular endoplasmic reticulum progressively increased during the periods of perfusion. These morphometric parameters from lungs in situ and isolated lungs suggest that changes occur in pulmonary surfactant synthesis and activity during perfusion. It is further postulated that progressive increases in the rates of surfactant removal and/or inactivation during perfusion may contribute to spontaneous edema in lungs isolated for periods exceeding two hours. The morphologic and physiologic integrity of isolated perfused lung preparations, widely used as models of lungs in vivo, in situ requires further evaluation.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001500110
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