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Structure Determination of Echovirus 1 (1998)

Filman, D. J. ; Wien, M. W. ; Cunningham, J. A. ; [et al.]

Copenhagen : International Union of Crystallography (IUCr)

Acta crystallographica 54 (1998), S. 1261-1272

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ISSN: 1399-0047

Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001

Topics: Chemistry and Pharmacology , Geosciences , Physics

Notes: The atomic structure of echovirus 1 (a member of the enterovirus genus of the picornavirus family) has been determined using cryo-crystallography and refined to 3.55 Å resolution. Echovirus 1 crystallizes in space group P22121 with a = 352.45, b = 472.15 and c = 483.20 Å. The crystals contain one full virus particle in the asymmetric unit allowing for 60-fold noncrystallographic symmetry averaging. The diffraction pattern shows strong pseudo-B-centering with reflections with h + l = 2n + 1 being systematically weak or absent below about 6 Å resolution. The size of the unit cell and presence of pseudo-B-centering placed strong constraints on the allowed packing of the icosahedral particle in the crystal lattice. These constraints greatly facilitated the determination of the orientation and position of the virus by reducing the dimensionality of the search, but interactions between the crystallographic and noncrystallographic symmetries rendered the choice of space group ambiguous until very late in the structure determination. This structure determination provides a striking example of the power of packing analysis in molecular replacement and illustrates how subtle interactions between crystallographic and noncrystallographic symmetries can be resolved.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1107/S0907444998002790

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Mycorrhizal infection and plant species diversity (1988)

BERGELSON, J. M. ; CRAWLEY, M. J.

[s.l.] : Nature Publishing Group

Nature 334 (1988), S. 202-202

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ISSN: 1476-4687

Source: Nature Archives 1869 - 2009

Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics

Notes: [Auszug] SIR-Grime et al.l argue that the presence of mycorrhizas leads to an increase in the plant species diversity of laboratory microcosms. They grew species-rich mixtures of plants with and without infection by mycorrhizal fungi, and found a small but significant increase in plant species diversity as ...