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  • 1
    Electronic Resource
    Electronic Resource
    Demonstration of immunoglobulin G with affinity for dopamine in cerebrospinal fluid from psychotic patients
    Amsterdam : Elsevier
    Clinica Chimica Acta 217 (1993), S. 129-142 
    Details
    ISSN: 0009-8981
    Keywords: Cerebrospinal fluid ; Dopamine ; Immunoglobulin G ; Psychotic patients
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0009-8981(93)90159-2
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  • 2
    Electronic Resource
    Electronic Resource
    Cryogenic linear ion trap for accurate spectroscopy (1996)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 67 (1996), S. 129-134 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: We have observed linear "crystals'' of up to tens of laser-cooled 199Hg+ ions in a linear rf ion trap. The trap operates at liquid-He temperature and is designed for use as a prototype 40.5 GHz frequency standard with high accuracy and stability. © 1996 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1146560
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  • 3
    Electronic Resource
    Electronic Resource
    Minimization of ion micromotion in a Paul trap (1998)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 83 (1998), S. 5025-5033 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Micromotion of ions in Paul traps has several adverse effects, including alterations of atomic transition line shapes, significant second-order Doppler shifts in high-accuracy studies, and limited confinement time in the absence of cooling. The ac electric field that causes the micromotion may also induce significant Stark shifts in atomic transitions. We describe three methods of detecting micromotion. The first relies on the change of the average ion position as the trap potentials are changed. The second monitors the amplitude of the sidebands of a narrow atomic transition, caused by the first-order Doppler shift due to the micromotion. The last technique detects the Doppler shift induced modulation of the fluorescence rate of a broad atomic transition. We discuss the detection sensitivity of each method to Doppler and Stark shifts, and show experimental results using the last technique.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.367318
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  • 4
    Electronic Resource
    Electronic Resource
    Isocitric dehydrogenase activity in non–inflamed attached gingiva of rhesus monkeys (1974)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 9 (1974), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Isocitric dehydrogenase activity was measured using ultramicrochemical techniques under optimum conditions in clinically non-inflamed attached gingival tissue of Rhesus monkeys. Only gingivectomy specimens determined to be clinically non-inflamed were used. Optimum assay conditions were determined in fresh gingival homogenates. Freeze-dried sections were dissected into morphologically distinct fragments which ranged in weight from 14 to 240 nanograms (10−9 grams). Isocitric dehydrogenase activities were calculated for the various layers of epithelium, whole epithelium and connective tissue. Mean activities were reported in micromoles of substrate converted per gram of dry tissue weight per minute at 37°C. They were: surface layer 31.38, granular layer 30.97, spinous layer 29.17, basal layer 53.19, connective tissue 32.23 and whole epithelium 30.19. These results are compatible with, though somewhat higher than isocitric dehydrogenase activities reported in similar epithelial tissues, such as normal human and prosimian epidermis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1974.tb00672.x
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  • 5
    Electronic Resource
    Electronic Resource
    Pentose shunt enzyme activities in non-inflamed attached gingiva of dogs (1973)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 8 (1973), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Ultramicrochemical methods were used to assay glucose-6-phosphate dehydrogenase (G-6-PDH) and 6-phosphogluconale dehydrogenase (6-PGDH) activities in non-inflamed attached gingiva of dogs, these enzymes being an integral part of the pentose shunt. Precautions were taken to assure the clinical non-inflamed status of the gingivectomy specimens. Frozen dried sections were dissected under a stereomicroscope into representative morphologically distinct fragments which ranged in weight from 12 to 240 nanograms (10-9 grams). Quantitative results for various strata of epithelium, connective tissue and whole epithelium were calculated. The mean combined G-6-PDH and 6-PGDH activities in micromoles of substrate converted., per gram of dry tissue per minute at 37° C. were: surface layer, 17.7; granular layer, 56.4; spinous layer, 58.1; basal layer, 49.5; connective tissue, 15.8; and whole epithelium, 46.6. The mean 6-PGDH activities in micromoles of substrate converted per gram of dry tissue per minute at 37° C. were: surface layer, 6.8; granular layer, 22.1; spinous layer, 17.5; basal layer, 20.8; connective tissue, 4.2; and whole epithelium, 16.9. The mean values for G-6-PDH activity were: surface layer, 10.9; granular layer, 34.4; spinous 40.6; basal 28.7; connective tissue, 11.6; and whole epithelium, 29.7. The results are compatible with the pattern of enzyme activity previously reported for primate skin (lm and Adachi, 1966) and rat palate (Gerson. 1967).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1973.tb00736.x
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  • 6
    Electronic Resource
    Electronic Resource
    Tensile strength, histological and immunohistochemical observations of periodontal wound healing in the dog (2002)
    Oxford, UK : Munksgaard International Publishers
    Journal of periodontal research 37 (2002), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: This study was designed to study wound strength at the dentine/connective tissue interface and at the bone/connective tissue interface following full thickness flap surgery. Flaps of uniform dimension were outlined in four young adult beagle dogs using a standardised double bladed knife and vertical incisions 10 mm apart, which extended 8 mm apical to the gingival margin. Bone was removed from half the sites (eight sites in each dog), giving 32 flaps replaced on dentine and 32 sites on bone. A tensile force was applied using a microprocessor force gauge at 1, 2, 3, 7, 10, 14, 21, and 28 days. Mean tensile strengths were markedly weaker for the dentine/flap interface. At 7 days the value for flaps to dentine was 1.82 N, in contrast to 5.08 N for flaps replaced on bone. Inflammatory cell counts tended to fall markedly at 3 days for both modalities, but were higher for the dentine/flap modality at all time points. Fibroblast density peaked at 7–14 days but did not vary with type of flap over the time points studied. The amounts of fibrin were greater for the dentine/flap interface at all time points but decreased for both flap types as time progressed. Collagen type V was localised to the basement membrane and blood vessels and tended to show more foci for flaps replaced on dentine. Procollagen levels showed little change over the healing interval for both flap/bone and flap/dentine interfaces. Type III collagen synthesis was at peak levels during the first week. These findings would support efforts to stabilise periodontal flaps at early time points, especially those on dentine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1600-0765.2002.01375.x
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  • 7
    Electronic Resource
    Electronic Resource
    Fructoaldolase activity in non-inflamed attached gingiva of rhesus monkeys (1973)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 8 (1973), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Ultramicrochemical methods were used to assay fructoaldolase activity under optimum substrate mixture concentrations in clinically non-inflamed attached gingival tissue of Rhesus monkeys. Precautions were taken to assure the clinical non-inflamed status of the gingivectomy specimens. Frozen dried sections were dissected under a stereomicroscope into representative morphologically distinct fragments which ranged in weight from 14 to 240 nanograms (10−9 grams). Optimum assay conditions for the microassay of freeze dried fragments were determined in fresh gingival homo-genates. Quantitative results for the various strata of epithelium, connective tissue and whole epithelium were calculated. The mean fructoaldolase activity was reported in micromoles of converted substrate per gram of dry tissue weight per minute at 37°C: surface layer 4·04, granular layer 15·57, spinous layer 23·58, basal layer 28·10, connective tissue 12·99 and whole epithelium 26·75. These results are higher than fructoaldolase activity reported in the literature for other epithelial tissue, such as skin of the parietal region of the scalp and the plantar surface of the foot. However, the results of the present investigation are compatible with the activity assayed for mucous membrane of the lip, a tissue less commonly involved in chronic inflammatory processes than is the gingival tissue assayed in this study.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1973.tb00761.x
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  • 8
    Electronic Resource
    Electronic Resource
    Lactic dehydrogenase in non-inflamed attached gingiva of dogs (1972)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 7 (1972), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Ultramicrochemical methods were used to assay lactic dehydrogenase (L.D.H.) activity in non- inflamed attached gingival tissue of dogs. Precautions were taken to insure the clinical and histological non-inflamed status of the gingivectomy specimens. Frozen dried sections were dissected under a stereomicroscope into representative morphologically distinct fragments which ranged in weight from 12 to 240 nanograms (10−9 grams). Quantitative results for the various strata of epithelium, connective tissue and whole epithelium were calculated. The mean L.D.H. activity was reported in micromoles per gram of dry tissue weight per minute at 37°C: surface layer, O; granular layer, 303; spinous layer, 613; basal layer, 801; connective tissue, 42; and whole epithelium, 574. These results are in agreement, for the most part, with L.D.H. activity assayed in other oral masticatory mucosal tissue, such as rat palate, which is less susceptible to chronic inflammatory process than is gingival tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1972.tb00625.x
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  • 9
    Electronic Resource
    Electronic Resource
    Hexokinase activity in non-inflamed attached gingiva of rhesus monkeys (1973)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 8 (1973), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Ultramicrochemical methods were used to assay hexokinase activity under optimum substrate mixture concentrations in clinically non-inflamed attached gingival tissue of Rhesus monkeys. Precautions were taken to assure the clinical non-inflamed status of the gingivectomy specimens. Frozen dried sections were dissected under a stereomicroscope into representative morphologically distinct fragments which ranged in weight from 14 to 240 nanograms (10−9 grams). Optimum assay conditions were determined in fresh gingival homogenates. Quantitative results for the various strata of epithelium, connective tissue and whole epithelium were calculated. The mean hexokinase activity was reported in micromoles of converted substrate per gram of dry tissue weight per minute at 37°C: surface layer 22.4. granular layer 22.6, spinous layer 18.9, basal layer 15.9, connective tissue 16.6 and whole epithelium 18.0. These results are compatible, for the most part, with hexokinase activity assayed in other epithelial tissue, such as skin of the parietal region of the scalp, plantar surface of the foot and mucous membrane from the lip, all of which are less commonly involved in chronic inflammatory processes than is gingival tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1973.tb00760.x
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  • 10
    Electronic Resource
    Electronic Resource
    Malic dehydrogenase in non-inflamed attached gingiva of dogs (1972)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 7 (1972), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Ultramicrochemical methods were used to assay malic dehydrogenase (M.D.H.) activity in non- inflamed attached gingiva of dogs. Precautions were taken to assure the clinical and histological non-inflamed status of the gingivectomy specimens. Frozen dried sections were dissected under a stereomicroscope into representative morphologically distinct fragments which ranged in weight from 12 to 240 manograms. Quantitative results for various strata of epithelium, connective tissue and whole epithelium were calculated. The mean M.D.H. activities in micromoles of substrate converted, per gram of dry tissue weight per minute at 37° C. were: surface layer, 29; granular layer, 222; spinous layer, 329; basal layer, 598; connective tissue, 71; and whole epithelium, 337. Differences in aerobic metabolism between the present findings in dogs and those reported for rat oral mucosa may in part be due to the type of keratinization in the two species. These differences in M.D.H. activity in dog and rat tissue are compatible with previosuly reported results of investigations of the oxygen consumption of these tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1972.tb01110.x
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