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DNA typing from single hairs (1988)

Higuchi, Russell ; von Beroldingen, Cecilia H. ; Sensabaugh, George F. ; [et al.]

[s.l.] : Nature Publishing Group

Nature 332 (1988), S. 543-546

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ISSN: 1476-4687

Source: Nature Archives 1869 - 2009

Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics

Notes: [Auszug] Most of the DNA in hair is located in the root and surrounding sheath cells11. As measured by the fluorescence of DNA-dye complexes in crude lysates of hairs, the root end of freshly-plucked hairs may contain as much as 0.5 jjig DNA, whereas hair shafts contain too little DNA to determine its ...

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1038/332543a0

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Eukaryotic transcription complexes (1984)

Beroldingen, Cecilia H. ; Reynolds, Wanda F. ; Millstein, Larry ; [et al.]

Springer

Molecular and cellular biochemistry 62 (1984), S. 97-108

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ISSN: 1573-4919

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Chemistry and Pharmacology , Medicine

Notes: Summary Accurate transcription of eukaryotic genes in vitro is preceeded by the assembly of the template DNA into stable DNA-protein complexes. Such complexes have been reported for genes transcribed by each of the three eukaryotic RNA polymerases. DNAase I protection or footprint assays have yielded information as to the sites of protein factor binding. These sites correlate with many of the sequences which have been implicated as promoter elements through analysis of deletion mutants. Stable transcription complexes are also formed in microinjected Xenopus oocytes and such complexes can be shown to exist in vivo in nuclei and chromatin. The propagation of active transcription complexes may prove to be a crucial element in cellular differentiation.