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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Bioprocess and biosystems engineering 11 (1994), S. 239-243 
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A strain isolated locally and identified as Rhizopus oryzae (RO, IIT KGP) was found to synthesise an extracellular enzyme, tanin acyl hydrolase, showing its degradability of tannic acid to gallic acid. For maximizing the enzyme secretion in the fermented broth, the influencing parameters were optimized in shake flask culture. Experiments showed that modified Czapek dox medium with 2% tannic acid, 1% glucose, 0.05% sodium nitrate incubated for 4 days with 2 days old inoculum was the optimum for the synthesis of tannase by Rhizopus oryzae (RO, IIT KGP). Maximum enzyme activity was found to be 6.12 U/ml.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Bioprocess and biosystems engineering 14 (1996), S. 159-162 
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract An attempt has been made to optimize the production of enzyme tannase by solid state fermentation (SSF) using the organism Rhizopus oryzae. The best favourable conditions for enzyme production include initial pH 5 with 4 days of incubation period at 40°C and 72% humidity, and 10 g wheat bran soaked in 2.5% tannic acid.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Bioprocess and biosystems engineering 12 (1995), S. 273-277 
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Biosorptions of Pb2+, Cr6+, Cd2+ and Ni2+ were investigated, with special emphasis on the first one, using live and dead fungal mycelia. Of the four fungi, namely Polyporus ostreiformis, Volvariella volvacea, Pleurotus sajor-caju and Phanerochaete chrysosporium, the last one was found to be most effective in Pb2+ removal. Total biosorption was effected in 6 days up to the Pb2+ concentration of 6 mg/l, with a specific uptake of 1.33 mg Pb2+/g dry cell mass. The removal of other three metals varied between 28.8–73.3% from a medium containing 4 mg/l of each of the metals.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Bioprocess and biosystems engineering 7 (1992), S. 225-228 
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Effect of additional chemicals at different culture conditions was studied in detail on newly isolated Rhizopus oryza (RO, IIT KGP) to maximize protease synthesis. The surfactants and metal ions when added to the basal medium individually were acted as inducers. The combined effect of these inducers on protease production was also studied in detail and a semiempirical mathematical model based on the data obtained was proposed to optimize their effects. A 2.5 fold increase in the protease production was possible by optimizing the two variable inducers system.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Bioprocess and biosystems engineering 13 (1995), S. 41-43 
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Production of proteolytic enzyme in a bubble column bioreactor was carried out using the microorganism Rhizopus oryzae. Optimization of the production conditions were done adopting the EVOP (evolutionary operation technique). Studies on two variable and three variable parameters were carried out. The variable parameters were air flow rate, aeration time and incubation period. 8 hours aeration time per day, 1.66 vvm air flow rate and 2 days incubation was found to be optimum.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Industrial & engineering chemistry research 27 (1988), S. 149-153 
    ISSN: 1520-5045
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Bioprocess engineering 11 (1994), S. 239-243 
    ISSN: 0178-515X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  A strain isolated locally and identified as Rhizopus oryzae (RO, IIT KGP) was found to synthesise an extracellular enzyme, tanin acyl hydrolase, showing its degradability of tannic acid to gallic acid. For maximizing the enzyme secretion in the fermented broth, the influencing parameters were optimized in shake flask culture. Experiments showed that modified Czapek dox medium with 2% tannic acid, 1% glucose, 0.05% sodium nitrate incubated for 4 days with 2 days old inoculum was the optimum for the synthesis of tannase by Rhizopus oryzae (RO, IIT KGP). Maximum enzyme activity was found to be 6.12 U/ml.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Bioprocess engineering 13 (1995), S. 41-43 
    ISSN: 0178-515X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  Production of proteolytic enzyme in a bubble column bioreactor was carried out using the microorganism Rhizopus oryzae. Optimization of the production conditions were done adopting the EVOP (evolutionary operation technique). Studies on two variable and three variable parameters were carried out. The variable parameters were air flow rate, aeration time and incubation period. 8 hours aeration time per day, 1.66 vvm air flow rate and 2 days incubation was found to be optimum.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Bioprocess and biosystems engineering 10 (1994), S. 241-250 
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Two types of airlift fermenters, conventional (UT-ALF) and modified (CDT-ALF) were investigated to evaluate their performance with respect to baker's yeast growth. The riser tube of conventional external loop airlift fermenter is replaced by a converging-diverging tube, which is named as modified airlift fermenter having downcomer to riser cross-sectional area ratio A d /A r =1.8. The results were compared for the two types of airlift fermenter. A modified growth kinetics model for baker's yeast with oxygen as limiting substrate, has been proposed. The values of K s and K d of the growth model were determined from experimental data. The proposed model represented better for CDT-ALF system compared to UT-ALF. Compared to UT-ALF, CDT-ALF always showed higher cell mass concentration and low residual sugar concentration irrespective of the operating conditions. At optimum operating condition (initial glucose concentration 30 g/l, air flow rate 0.5 vvm and fermentation time 8 hrs.) 16.7% higher cell mass was observed in CDT-ALF compared to that in UT-ALF and yield (Y x/s ) was found to be 0.51 which was theoretically very near to maximum achievable value.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Bioprocess and biosystems engineering 7 (1992), S. 369-374 
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A newly isolated Rhizopus oryzae was found to exhibit some unusual phenomenon of secreting alkaline protease which was purified and characterized. The molecular weight was determined to be 28,600 dalton in gel electrophoresis. The enzyme is stable in the pH range from 3 to 11 and most active at pH 8. The temperature optimum of this thermostable biocatalyst is at 60 °C. The enzyme is sensitive to metal chelators, most of the metal ions (excepting a few monovalent cations) and inhibitor like PMSF. This indicates that the protease of isolated Rhizopus oryzae falls under alkaline serine group.
    Type of Medium: Electronic Resource
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