Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Intrapopulation Recombination for 15N-Determined Dinitrogen Fixation Ability in Common Bean (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 106 (1991), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The quantity of N2 fixed in common bean (Phaseolus vulgaris L.) is a quantitatively inherited trait that shows sufficient genetic variability for improvement through breeding, but there is little information on gain from selection for 15N-determined N2 fixation ability. The objective was to determine whether intrapopulation recombination and selection could be used to improve N2 fixation. Four agronomically acceptable lines were selected, based on their acetylene reduction-determined N2 fixation ability, from an inbred backcross (IB) line population created from a cross between the high N2-fixing donor parent ‘Puebla 152’ and a commercial, low N2-fixing recurrent parent ‘Sanilac’. These four IB lines were intercrossed, advanced to the F3, and the six resulting F3 populations with 25 F3 families per population were evaluated for N2 fixation in a low-N field using 15N-depleted (NH4)2SO4. The six F3 population means differed significantly and were superior to ‘Sanilac’ for N2 fixation ability and yield, indicating significant improvement over ‘Sanilac’. Four F3 population means were similar to ‘Puebla 152’, indicating selection advance towards high N2 fixation while retaining favorable agronomic characteristics. Fifty percent of the F3 families were superior to ‘Sanilac’ for N2 fixation and one family exceeded ‘Puebla 152’, indicating that intrapopulation recombination produced improvement of 15N-determined N2 fixation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1991.tb00504.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Selection for Increased Seed Nitrogen Accumulation in Common Bean: Implications for Improving Dinitrogen Fixation and Seed Yield (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 106 (1991), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Estimates of N2 fixation in segregating populations of bean plants based on 15N-isotope methods are technically demanding and expensive; therefore, indirect measures based on related traits including total seed N were used to select for improved N2 fixation and yield. In 1985, six populations of F2-derived F3 families resulting from six parental lines crossed to a common tester were grown in field trials on a low-N soil. In 1986, 25 selected half-sib families and two populations of full-sib F4 families were grown under similar conditions. Parents and a non-nodulating soybean line were included both years. Narrow sense (HNS) heritability estimates based on parent-offspring regressions ranged from 0.57 for total seed N to 0.39 for shoot biomass in one population, but were near zero for all measured traits in a second population. Among the criteria used to identify parental lines with superior potential for producing progenies with high total seed N, testcross population means combined with estimates of realized heritability were the most reliable. Selection of the best F3 families for total seed N resulted in F4 families with increased total seed N and higher seed yields, while seed protein percentage was unchanged. When plants are grown on low-N soils, selection for total seed N offers a useful alternative to selection for increased N2 fixation based on 15N-isotopic or total-N-difference method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1991.tb00515.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Heritable variation in the phaseolin protein of nondomesticated common bean, Phaseolus vulgaris L. (1985)
    Springer
    Theoretical and applied genetics 71 (1985), S. 478-480 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus vulgaris L. ; Phaseolin ; Seed-protein ; Genetic variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Crude protein extracts from single seeds of nondomesticated Mexican bean accessions were analysed by SDS polyacrylamide gel electrophoresis for variability in phaseolin protein. Six new phaseolin types; ‘M1’, ‘M2’, ‘M3’, ‘M4’, ‘M5’, ‘M6’, which contained polypeptides within the same range of molecular weights (51,000 to 45,000 daltons) as occur in the ‘S’, ‘T’ and ‘C’ phaseolin types of cultivated beans were identified. No ‘T’ and ‘C’ types were found among the non-domesticated Mexican accessions, and the ‘S’ type occurred in less than 7% of the seeds screened. Genetic analyses of F2 progenies from crosses between ‘Sanilac’ (‘S’), and five of the ‘M’ types showed that each ‘M’ phaseolin phenotype was allelic to the ‘S’ type and expressed codominantly.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00251191
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Genetic variation in the subunits of globulin-1 storage protein of French bean (1981)
    Springer
    Theoretical and applied genetics 59 (1981), S. 83-88 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus vulgaris ; Storage proteins ; Electrophoresis ; Genetic variation ; Banding types
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Charge and molecular weight heterogeneity of globulin-1 (G1) polypeptides of the bean, Phaseolus vulgaris L., were revealed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Different bean cultivars were classified into three groups: ‘Tendergreen’, ‘Sanilac’, and ‘Contender’ on the basis of their protein subunit composition. Nine distinct major bands: α51,α49, α48.5,β48T, β48S, β47, γ45.5, γ45S, and γ45C, and two minor bands: γ46T and γ46S were found to account for the three profiles seen on one-dimensional SDS-PAGE. Two-dimensional analysis revealed these eleven protein bands to be composed of a minimum of fourteen distinct protein subunits. The ‘Tendergreen’ and ‘Sanilac’ types differ in their G1 polypeptide composition. The protein patterns of the ‘Contender’ types are intermediate, containing many protein subunits found in the patterns of the ‘Tendergreen’ and ‘Sanilac’ types suggesting a genetic and evolutionary relationship.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00285895
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Linkage relationships between genes controlling seed proteins in French bean (1981)
    Springer
    Theoretical and applied genetics 60 (1981), S. 251-259 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus vulgaris ; Phaseolin ; Seed proteins ; Electrophoresis ; Linkage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The inheritance of phaseolin and globulin-2 (G2)/albumin polypeptides was investigated in crosses involving varieties which exhibited the three electrophoretic banding patterns of phaseolin found in French bean. ‘Total’ seed protein extracts of single seeds of the F1 and F2 generations from the crosses: ‘Sanilac’ × ‘Contender’, ‘BBL 240’ × ‘Contender’, and ‘Sanilac’ × ‘BBL 240’ were analyzed by two-dimensional electrophoresis. Segregation of the genes controlling phaseolin and G2/albumin polypeptides, and those controlling a further five groups of seed proteins (A, B, D, E, and F) were observed. No recombinant electrophoretic phenotypes were seen for phaseolin or G2/albumin polypeptides suggesting that the genes controlling each of these groups of polypeptides are closely linked and segregate like single Mendelian genes. The phaseolin genes and G2/albumin genes were not linked to each other. The group of genes controlling phaseolin polypeptides were linked to those controlling group B proteins, and those controlling G2/albumin polypeptides were linked to those controlling group F proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02342545
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Bean lectins (1982)
    Springer
    Theoretical and applied genetics 62 (1982), S. 263-271 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus vulgaris ; Seed protein ; Lectins ; Electrophoresis ; Agglutination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Single seeds of over 100 bean cultivars were analyzed by two-dimensional electrophoresis. The cultivars could be classified into eight groups by virtue of their G2/albumin electrophoretic patterns: TG2, SG2, VG2, PrG2, BG2, MG2, PG2, and PiG2, The polypeptide compositions of these types were largely inter-related having particular polypeptides in common. It was possible to correlate the G2/albumin patterns with agglutinating activity of cow and rabbit blood cells as measured by the agglutination ratio (minimum concentration of extract required to agglutinate cow blood cells: minimum concentration of extract required to agglutinate rabbit blood cells). The active lectin polypeptides were identified by extracting lectins from agglutinated erythrocytes and by comparing the qualitative similarities and differences of the G2/albumin patterns and their agglutination activities. A reference catalogue of over 100 bean cultivars giving their phaseolin and G2/albumin electrophoretic patterns, and agglutination ratios is presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276249
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Bean lectins (1982)
    Springer
    Theoretical and applied genetics 62 (1982), S. 361-367 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus vulgaris ; Lectins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The relationship between the polypeptide composition and the agglutination behaviour of the lectin-containing G2/albumin protein groups has allowed the identification of the active lectin polypeptides in different cultivars of Phaseolus vulgaris (Brown et al. accompanying paper). These results were used to ascertain the particular G2/albumin group contained in the various lectin sources used previously for the purification of lectin proteins. Many studies were found to have included lectin sources which contained the same G2/albumin pattern (TG2) and this common denominator has permitted the direct comparison of the properties reported for these purified lectins. Thus, much of the extensive literature on bean lectins is concurred.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00275105
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Enhanced available methionine concentration associated with higher phaseolin levels in common bean seeds (1984)
    Springer
    Theoretical and applied genetics 69 (1984), S. 47-53 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus vulgaris L. ; Streptococcus zymogenes bioassay ; Rocket immunoelectrophoresis ; Micro-Kjeldahl ; Limiting essential amino acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The relationship between available methionine concentration and the levels of phaseolin — the major seed storage proteins of the common bean — was studied using three groups of genetic materials: First, the F2 progenies of interspecific crosses between P. vulgaris cultivars and aP. coccineus subsp. coccineus line (cv. ‘Mexican Red Runner’) having no detectable phaseolin; second, the F2 progenies and segregating F3 families of crosses between cultivated P. vulgaris lines and a Mexican wild bean accession (PI 325690-3) carrying a gene producing a reduction in phaseolin content; third, two inbred backcross populations: ‘Sanilac’x‘Bush Blue Lake 240’ (population 2) and ‘Sanilac’x‘15R 148’ (population 6). Total seed N levels were determined by micro-Kjeldahl, phaseolin levels by rocket immunoelectrophoresis and available methionine levels by the Streptococcus zymogenes bioassay. Our results indicate that in all the genetic materials studied, with the exception of population 6, higher phaseolin levels lead to increased available methionine concentration. Although phaseolin has a low methionine concentration, it is actually a major source of available methionine in common bean seeds, because it represents a large part of total seed nitrogen and because limited differences exist between the methionine concentrations of the different protein fractions. This contrasts with the situation in cereals such as maize, barley and sorghum, where increased levels of the major limiting amino acid (lysine) can be achieved through a decrease in the amounts of the main seed storage protein fraction (prolamines). In population 6, no relationship was observed between available methionine and phaseolin content. Other factors, such as additional methionine-rich polypeptides or the presence of tannins, might obscure the positive relationship between phaseolin and available methionine content in population 6.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00262537
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Bean lectins (1985)
    Springer
    Theoretical and applied genetics 70 (1985), S. 22-31 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus vulgaris ; Lectin ; Phaseolin ; Quantitative variation ; Immunoelectrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Seeds of forty bean cultivars having different lectin types based on two-dimensional isoelectric focusing-sodium dodecyl sulfate polyacrylamide gel electrophoresis (IEF-SDS/PAGE) were analyzed for quantities of lectin, phaseolin and total protein. Significant differences were found among groups of cultivars with different lectin types for the quantity of lectin and phaseolin. Cultivars with more complex lectin types based on IEF-SDS/PAGE tended to have higher quantities of lectin and lower quantities of phaseolin than cultivars with simple lectin types. An association between lectin type and the quantity of lectin and phaseolin was found also in the seeds of F2 plants that segregated in a Mendelian fashion for two lectin types. Seeds from plants with the complex lectin type had more lectin and less phaseolin than seeds from plants with the simple lectin type. Therefore, the genes controlling qualitative lectin variation also may influence the quantitative variation of lectin and phaseolin. The results of this study are related to other studies on the quantitative variation for seed proteins and to the possible molecular basis for variation in the quantity of lectins in beans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264478
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Bean arcelin (1986)
    Springer
    Theoretical and applied genetics 71 (1986), S. 847-855 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus vulgaris ; Seed protein ; Arcelin ; Inheritance ; Linkage ; Bruchidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Crude proteins from seeds of wild bean accessions of Mexican origin were analyzed by one-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS/PAGE). Several accessions had electrophoretic patterns showing unique protein bands. When analyzed by two-dimensional isoelectric focusing (IEF)-SDS/PAGE, four protein variants which had electrophoretic mobilities similar to each other but different from the other major seed proteins, phaseolin and lectin, were observed. All four variants, which have not been described in cultivated beans, were tentatively named arcelin proteins and designated as arcelin 1, 2, 3 and 4. Arcelins 3 and 4 had polypeptides that comigrated on two-dimensional gels and these variants occurred in accessions that were collected in the same location. Analysis of single F2 seeds from crosses among arcelin-containing lines and from crosses between cultivated beans lines without arcelin and arcelin-containing lines revealed that differences in arcelin polypeptide expression were inherited monogenically. The alleles for different arcelin variants were codominant to each other and dominant to the absence of arcelin. The gene(s) controlling arcelin proteins were unlinked to those controlling phaseolin expression and tightly linked to genes controlling the presence of lectin proteins (〈 0.30% recombination). The possible origins of arcelin genes and their potential role in bruchid resistance are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276428
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...