ISSN:
0006-3592
Keywords:
96 well plate reader
;
microtiter plate
;
enzyme kinetics
;
Km
;
Kmax
;
enzyme assays
;
acetylcholinesterase monitoring
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
A method is described for the routine determination of kinetics parameters from colorimetric enzyme reactions using a 96-well microtiter plate reader commonly used in immunoassay. A program in Turbo Pascal have been developed for the analysis of results. The system performs the description of plate format, the definition of conditions of assays, the measurement of enzyme reactions and collection of data, analysis, visualization of results and modification of data. Measurement of Km and Vmax is made with different methods, including iterative determination, Lineweaver-Burk transformation, and Hofster transformation, to give the final values of kinetic parameters with their associated standards errors. This approach should be applicable to routine determination of kinetic parameters.
Additional Material:
1 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bit.260380413
Permalink
