Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Rapid assessment of metabolic activity in marine microalgae: application in ecotoxicological tests and evaluation of water quality (1992)
    Springer
    Marine biology 112 (1992), S. 199-205 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new method for the assessment of the effects of several contaminants on marine microalgae,Tetraselmis suecica (Kyling) Butcher,Skeletonema costatum (Grev.) Cleve, andProrocentrum lima (Ehrenberg) Dodge was developed in 1990. The method is based on the measurement of cell esterase activity using a fluorimetric stain, fluorescein diacetate (FDA), selected from amongst three stains (FDA, Neutral Red, thiazolyl tetrazolium bromure) for its higher sensitivity. Biochemical (K m,V max) and physiological (growth, specific activity) aspects of the enzymatic activity as revealed by the FDA method are discussed. Different categories of compounds (weed-killers, insecticides, metals) and some water samples from Seine Bay were tested for their toxic effects on microalgae. Experiments were performed on microplates using a fluorimetric microplate reader. The various steps of the experiments and data-processing were controlled by software. Applications of the system to rapid ecotoxicological tests (determination of the IC50, i.e., the concentration at which 50% inhibition of growth occurs) and to the assessment of environment quality by studying the toxic effect of water samples on microalgae are proposed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00702462
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Routine determination of enzyme kinetics using Plate reader (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 38 (1991), S. 434-437 
    Details
    ISSN: 0006-3592
    Keywords: 96 well plate reader ; microtiter plate ; enzyme kinetics ; Km ; Kmax ; enzyme assays ; acetylcholinesterase monitoring ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A method is described for the routine determination of kinetics parameters from colorimetric enzyme reactions using a 96-well microtiter plate reader commonly used in immunoassay. A program in Turbo Pascal have been developed for the analysis of results. The system performs the description of plate format, the definition of conditions of assays, the measurement of enzyme reactions and collection of data, analysis, visualization of results and modification of data. Measurement of Km and Vmax is made with different methods, including iterative determination, Lineweaver-Burk transformation, and Hofster transformation, to give the final values of kinetic parameters with their associated standards errors. This approach should be applicable to routine determination of kinetic parameters.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260380413
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...