ISSN:
1432-2307
Keywords:
Keywords Small round cell tumors
;
Ewing’s sarcoma
;
Translocation
;
Immunohistochemistry
;
Differential diagnosis
;
RT-PCR
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract It is now widely accepted that the EWS/FLI-1 fusion transcript is associated with tumors of the Ewing family. To test whether it is possible to detect the fusion transcript by means of combining polymerase chain reaction (PCR) methodology and immunohistochemistry, we investigated tumors of the Ewing family using in situ reverse transcriptase (RT)-PCR. We were able to demonstrate the t(11;22) fusion transcript in five of six cases of Ewing’s sarcoma and four of four peripheral primitive neuroectodermal tumors. These results were confirmed using fluorescence in situ hybridization in seven tumor samples. In situ RT-PCR-labeled fusion transcripts were found in virtually all tumor cells within a given sample, indicating that each cell possessed the t(11;22) transcript. We conclude from these results that in situ RT-PCR can be used for the rapid detection of EWS/FLI-1 fusion transcripts in biopsy material. The findings also suggest that all cells of the tumors of the Ewing family carry the EWS/FLI-1 fusion transcript.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004280000252
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