ISSN:
1573-2657
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary Guinea pig mesenteric microarteries (diameter 60–100 μm), the main branch of the mesenteric artery and taenia coli were skinned with 1% Triton X-100 for 4 h at 4°C. Microarteries, mounted for circumferential force measurement, developed maximal active force in response to elevation of the free Ca2+ (pCa=4.52, in EGTA buffer) in the presence of ATP (7.5 mM) and calmodulin (0.1–0.3 μM). In these preparations, addition of phalloidin (1–100 μM) slowly (〉1 h) relaxed submaximal contractions (pCa〉4.52) in a dose-dependent manner. Relaxation was irreversible as, after phalloidin wash-out, subsequent active force to pCa=4.52 was also reduced. By contrast, phalloidin preincubation and wash-out under relaxed conditions (pCa〉8) only reduced subsequent force to pCa=4.52 on prolonged stimulation. The extent of phalloidin-induced relaxation was not dependent on free Ca2+ between pCa 6.40 and 4.52. Phalloidin-induced relaxation did not occur during rigor contractions (i.e. absence of ATP and Ca2+). These mechanical effects of phalloidin were accompanied by a decreased leak of actin out of the skinned preparations and by the prevention of guanidine extraction of actin from these preparations. Phalloidin did not inhibit the myosin light chain kinase or phosphatase activity isolated from these preparations. In addition, the relaxant effects were also noted in taenia coli and the main branch of the mesenteric artery but not in skinned porcine ventricular heart muscle. These experiments suggest the possible participation of actin filament dynamics on the maintenance of active force in Triton-skinned smooth muscle.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01738430
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