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Plastid photodamage and Cab gene expression in barley leaves (2000)

La Rocca, Nicoletta ; Dalla Vecchia, Francesca ; Barbato, Roberto ; [et al.]

Copenhagen : Munksgaard International Publishers

Physiologia plantarum 109 (2000), S. 0

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ISSN: 1399-3054

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: The effects of norflurazon (NF) and amitrole (AM), two bleaching herbicides which inhibit carotenogenesis, were compared in leaves of 7-day-old barley (Hordeum vulgare L. cv Express) plants grown in damaging light. The herbicide effects were analysed with respect to chloroplast organization, photosynthetic functionality and nuclear photodependent expression of the Lhcb1 gene, which codes for the Lhcb1 light-harvesting chlorophyll a/b binding protein of photosystem II. Both herbicides caused dramatic photooxidation of organelles, which were photosynthetically unfunctional. Plastids of NF-treated plants lacked thylakoids and pigments. Plastids of AM-treated plants had some strikingly altered membranes and contained only very small quantities of chlorophylls. Despite the presence of severely photodamaged plastids, cells of AM-treated leaves contained high levels of Lhcb1 transcript. This transcript, on the contrary, was completely absent in the cells of NF-treated plants. These findings suggest that in order to block expression of nuclear genes coding for plastid-resident proteins, photodamage leading to the complete dismantling of thylakoids and to the total absence of any form of photosynthetic pigment is required.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1034/j.1399-3054.2000.100108.x

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A simple colorimetric method for detecting cell viability in cultures of eukaryotic microorganisms (1982)

Bonora, Angelo ; Mares, Donatella

Springer

Current microbiology 7 (1982), S. 217-221

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ISSN: 1432-0991

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Abstract The dye methylene blue can be taken up by dead or severely damaged cells, but not by living cells. Based on this fact, a method was devised which permits quantitative determinations of injured cells in populations of microorganisms such asSaccharomyces cerevisiae, Rhodotorula glutinis, andEuglena gracilis. The percentage of damaged cells was determined by measuring, at 664 nm, the optical density of cell suspensions pretreated with 0.15 mM methylene blue for 6 min, a condition that does not affect cell integrity as determined by oxygen consumption and release of potassium ions. This technique is faster and simpler than the classical dye-exclusion and plate-counting methods.