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  • 1
    Electronic Resource
    Electronic Resource
    Expression of a dominant-negative Ras mutant does not affect stimulation of glucose uptake and glycogen synthesis by insulin (1996)
    Springer
    Diabetologia 39 (1996), S. 558-563 
    Details
    ISSN: 1432-0428
    Keywords: Glucose uptake ; glycogen synthesis ; Rasmediated signalling ; phosphatidylinositol-3 kinase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary It has previously been shown that insulin-induced stimulation of glucose uptake and glycogen synthesis requires activation of phosphatidylinositol-3-kinase (PI3kinase). Insulin also induces formation of RasGTP in cells and various studies have yielded inconsistent data with respect to the contribution of signalling pathways activated by RasGTP, to insulin-stimulated glucose uptake and glycogen synthesis. We have examined the requirement of RasGTP-mediated signalling for these insulin responses by expression of a dominant negative mutant of Ras (RasN17) in cells by vaccinia virus mediated gene transfer. This Ras-mutant abrogates the signalling pathways mediated by endogenous RasGTP. Subsequently, the ability of insulin to stimulate 2-deoxyglucose uptake and glycogen was examined. We observed that expression of RasN17 in 3T3L1 adipocytes did not affect the stimulation of hexose uptake by insulin. Similarly, expression of RasN17 in A14 cells, an NIH 3T3-derived cell line with high expression of insulin receptors, did not affect insulin-induced stimulation of glycogen synthesis. In both cell lines, insulin-induced phosphorylation of Mapkinase (Erk1,2) was abrogated after expression of RasN17, demonstrating the functional interference by RasN17 with signalling mediated by endogenous RasGTP. Wortmannin, an inhibitor of PBkinase, abolished dose-dependently the insulin-induced stimulation of hexose uptake and glycogen synthesis without an effect on RasGTP levels in both cell types. We conclude that stimulation of glucose transport and glycogen synthesis by insulin occurs independently of RasGTP-mediated signalling.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00403302
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  • 2
    Electronic Resource
    Electronic Resource
    Expression of a dominant-negative Ras mutant does not affect stimulation of glucose uptake and glycogen synthesis by insulin (1996)
    Springer
    Diabetologia 39 (1996), S. 558-563 
    Details
    ISSN: 1432-0428
    Keywords: Keywords Glucose uptake ; glycogen synthesis ; Ras-mediated signalling ; phosphatidylinositol-3 kinase.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary It has previously been shown that insulin-induced stimulation of glucose uptake and glycogen synthesis requires activation of phosphatidylinositol-3-kinase (PI3kinase). Insulin also induces formation of RasGTP in cells and various studies have yielded inconsistent data with respect to the contribution of signalling pathways activated by RasGTP, to insulin-stimulated glucose uptake and glycogen synthesis. We have examined the requirement of RasGTP-mediated signalling for these insulin responses by expression of a dominant negative mutant of Ras (RasN17) in cells by vaccinia virus mediated gene transfer. This Ras-mutant abrogates the signalling pathways mediated by endogenous RasGTP. Subsequently, the ability of insulin to stimulate 2-deoxyglucose uptake and glycogen was examined. We observed that expression of RasN17 in 3T3L1 adipocytes did not affect the stimulation of hexose uptake by insulin. Similarly, expression of RasN17 in A14 cells, an NIH 3T3-derived cell line with high expression of insulin receptors, did not affect insulin-induced stimulation of glycogen synthesis. In both cell lines, insulin-induced phosphorylation of Mapkinase (Erk1,2) was abrogated after expression of RasN17, demonstrating the functional interference by RasN17 with signalling mediated by endogenous RasGTP. Wortmannin, an inhibitor of PI3kinase, abolished dose-dependently the insulin-induced stimulation of hexose uptake and glycogen synthesis without an effect on RasGTP levels in both cell types. We conclude that stimulation of glucose transport and glycogen synthesis by insulin occurs independently of RasGTP-mediated signalling. [Diabetologia (1996) 39: 558–563
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00403302
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  • 3
    Electronic Resource
    Electronic Resource
    Close linkage of a highly polymorphic marker (D5S37) to familial adenomatous polyposis (FAP) and confirmation of FAP localization on chromosome 5q21-q22 (1988)
    Springer
    Human genetics 〈Berlin〉 79 (1988), S. 183-185 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Fifteen apparently unrelated Dutch families with familial adenomatous polyposis (FAP) also known as familial polyposis coli (FPC; McKusick No. 17510) were screened for linkage with the DNA probe C11p11 localized on chromosome 5q21–22 and previously reported to be closely linked to FAP (Bodmer et al. 1987; Leppert et al. 1987). In our study C11p11 was minimally informative, which is ascribable to its low heterozygosity in the North European populations. Of the above families, 12 were investigated also for linkage with D5S37 (DNA probe Pi227). Data from 11 of them were found to be informative and showed that FAP is closely linked to D5S37 previously localized on chromosome 5q21 (peak lod score 7.85 at a recombination fraction of 0.048 with 95% probability limits 0.005–0.145). Results discussed below indicate for the first time that the most likely location of the FAP gene is in the band 5q22 very close to 5q21, if not in the transitional zone between these two bands. The probe Pi227 recognizes 4 restriction fragment length polymorphism (RFLP) sites, exhibiting a total of 9 alleles with 24 theoretically possible haplotypes in the Dutch population. Therefore, this probe appears to have potential as a generally useful predictive marker for FAP until much closer and similarly useful markers become available.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00280563
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  • 4
    Electronic Resource
    Electronic Resource
    Immunocompetent cells in psoriasis (1983)
    Springer
    Archives of dermatological research 275 (1983), S. 181-189 
    Details
    ISSN: 1432-069X
    Keywords: Psoriasis ; Immunocompetent cells ; T-cell subsets ; Monoclonal antibodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Immunocompetent cells in exacerbating untreated psoriasis vulgaris skin lesions were immunophenotypically studied by the application of a selection of monoclonal antibodies in a two-stage immunoperoxidase technique. Epidermal changes include: focal accumulation of immunoglobulins in the stratum corneum, as demonstrated by a mixture of monoclonal anti-kappa and anti-lambda antibodies; focal accumulation of OKM-1 positive but Mo-2 negative cells high in the epidermis, reflecting granulocytes in Munro's abcesses; a marked decrease in epidermal Langerhans cells with focal abnormal clumping and smaller dendrites, as demonstrated by monoclonal anti-HLA-DR and anti-T6 (OKT-6) antibodies; and, sporadic exocytosis of mainly T1 (Leu-1), T8 (Leu-2a) positive suppressor/cytotoxic T lymphocytes. The dermal infiltrates were found to consist mainly of partically activated T1 (Leu-1), T4 (Leu-3a) positive T-helper/inducer cells with a smaller compartment of T1 (Leu-1), T8 (Leu-2a) positive suppressor/cytotoxic lymphocytes. These cells were found in close apposition to T6 (OKT-6), HLA-DR positive Langerhans cells and further accompanied by a minor compartment of OKM-1, Mo-2 positive monocytes. No B-cells or plasma cells could be demonstrated in the dermis. Natural killer cells were observed only incidentally. These results fit best with the hypothesis that psoriasis is a chronic inflammatory condition as a result of persistant stimulation of T cells by immunogen(s) of epidermal origin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00510050
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  • 5
    Electronic Resource
    Electronic Resource
    Quantitation of soluble CD27, a T-cell activation antigen, and soluble interleukin-2 receptor in serum from patients with psoriasis (1991)
    Springer
    Archives of dermatological research 283 (1991), S. 533-534 
    Details
    ISSN: 1432-069X
    Keywords: Psoriasis ; CD27 ; Interleukin-2 receptor ; Disease marker
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00371929
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  • 6
    Electronic Resource
    Electronic Resource
    Subacute zinc deficiency in children with recurrent upper respiratory tract infection (1987)
    Springer
    European journal of pediatrics 146 (1987), S. 293-295 
    Details
    ISSN: 1432-1076
    Keywords: Zinc- serum- urine- hair ; Recurrent respiratory tract infection ; Height for age
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Serum, urine and hair zinc levels in 20 patients with recurrent upper respiratory tract infection are compared with those of age- and sex-matched controls. Lower hair zinc (1.44 vs 2.00 mmol/g hair,P=0.004) and higher urinary zinc levels (2.2 vs 1.6 mmol/mol creatinine,P=0.05) were found, but no difference in serum zinc. The patients had lowernormal height for age (SD-score 0.2 vs 0.7,P=0.031), there was no difference in weight for height. No correlation was found between the zinc values and the duration of the complaints.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00716478
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  • 7
    Electronic Resource
    Electronic Resource
    Possible radiation-induced temperature effects on chemical reactions in organic glasses
    Amsterdam : Elsevier
    International Journal of Radiation Applications & Instrumentation. Part C, 34 (1989), S. 361-367 
    Details
    ISSN: 1359-0197
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/1359-0197(89)90245-2
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  • 8
    Electronic Resource
    Electronic Resource
    Radiation-generated cations of 1,1-diphenylethylene and their kinetic behaviour at low temperatures
    Amsterdam : Elsevier
    International Journal of Radiation Applications & Instrumentation. Part C, 36 (1990), S. 715-719 
    Details
    ISSN: 1359-0197
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/1359-0197(90)90167-G
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  • 9
    Electronic Resource
    Electronic Resource
    Radiation-induced anionic species of maleic anhydride in glassy matrices
    Amsterdam : Elsevier
    International Journal of Radiation Applications & Instrumentation. Part C, 39 (1992), S. 197-202 
    Details
    ISSN: 1359-0197
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/1359-0197(92)90041-D
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  • 10
    Electronic Resource
    Electronic Resource
    Clinical improvement in chronic plaque-type psoriasis lesions after narrow-band UVB therapy is accompanied by a decrease in the expression of IFN-γ inducers – IL-12, IL-18 and IL-23 (2004)
    Oxford, UK; Malden, USA : Munksgaard International Publishers
    Experimental dermatology 13 (2004), S. 0 
    Details
    ISSN: 1600-0625
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract:  Type-1 cytokine-producing T cells are important in the pathogenesis of psoriasis vulgaris, for which efficient therapy is provided by means of narrow-band ultraviolet-B (NB-UVB). The expression of the type-1 cytokine interferon-γ (IFN-γ) is regulated by interleukin-12 (IL-12), IL-15, IL-18 and IL-23; however, not much is known about the effect of this therapy on the levels of these cytokines in lesional psoriatic skin in situ. In this study, we investigated the effects of NB-UVB therapy on the expression of IFN-γ-inducing cytokines. Ten patients with chronic plaque-type psoriasis selected to be treated with NB-UVB therapy were recruited for these experiments and the expression of cytokines IL-12, IL-15, IL-18, IL-23 and IFN-γ in lesional psoriatic skin before, during and after therapy was determined with the help of immunohistochemistry. Double staining was performed in order to determine the cell types expressing these cytokines. The decrease in the psoriasis area and severity index was accompanied by a significant decrease in the expression of IFN-γ, and concomitantly, significant reduction of IFN-γinducers – IL-12, IL-18 and IL-23. Thus, we concluded that the decrease of IFN-γ expression in psoriasis lesions after NB-UVB therapy could be a result of diminished expression of IL-12, IL-18 and IL-23 in lesional skin. Therapies targeting these three cytokines should, therefore, be considered in the treatment of psoriasis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.0906-6705.2004.00246.x
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