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  • 1
    ISSN: 1573-4935
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Muscle tissue levels of dl-α-tocopherol (vitamin E) were significantly lower in endurance-trained rats than in sedentary animals, whether the animals were fed on vitamin-E-deficient or control (vitamin-E-sufficient) diets. In vitamin-E-deficient rats, liver tissue levels of vitamin E were significantly lower in those that were endurance-trained than in those that were sedentary; this was not the case in control animals. In addition, for vitamin-E-deficient rats, the onset of red-blood-cell hemolysis in the sedentary animals occurred one week earlier than in the endurance-trained animals. Thus, it appears that training induces a protective effect against hemolysis despite vitamin E deficiency.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-4935
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Oxidation of pyruvate, α-ketoglutarate, palmitoylcarnitine, succinate, and ferrocytochrome c by interscapular-brown-adipose-tissue (BAT) mitochondria of untrained and trained rats were measured at rest and alter running to exhaustion. At rest, BAT mitochondria from trained rats showed significantly lower activities (〈50%) for the oxidation of all the substrates. In untrained rats the activities of the enzymes for the oxidation of all the substrates except pyruvate and succinate were lower at exhaustion compared to the resting state when expressed on a per-gram-fresh-Weight basis. In trained rats all of the enzyme activities increased as a result of exhaustive exercise. These differences between the two groups of rats in the post-exercise changes in oxidative capacities suggest that following an initial adaptation, resulting in a large decrease in mitochondrial oxidative activity, training protects the residual oxidative pathways against exercise-induced inactivation. These data show that unlike exposure to cold, or overfeeding, a physiological stimulus such as exercise reduces the oxidative capacity of BAT, and therefore may reduce the thermogenic activity of the tissue in endurance-trained rats as has been addressed in the scientific literature.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    European journal of applied physiology 32 (1974), S. 183-186 
    ISSN: 1439-6327
    Keywords: Muscle ; Temperature ; Thermocouple ; Heat Exertion ; Q 10
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract An improved method of quadriceps thermocouple implantation utilizing a commercially available teflon venous catheter is described. This catheter possesses an inner protruding steel needle, which can be withdrawn after catheter implantation, and a leur fitting which allows syringe attachment and infusion of pain blocker during catheter implantation. Compared to other methods of thermocouple implantation, the present method appears to minimize discomfort to subjects, significantly increases thermocouple longevity, and permits thermocouple replacement during exercise. We believe this technique will facilitate research involving the continuous measurement of muscle temperature during exercise.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 11 (1984), S. 557-561 
    ISSN: 0306-042X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Natural differences in 13C/12C ratios of various metabolic fuels can produce systematic changes in the 13C/12C ratio of breath CO2, and therefore introduce errors into 13CO2 breath tests. To gain insight into the potential problem, we compared 13C/12C ratios of plasma macronutrients to those of breath CO2 under conditions that should alter the percentages of carbohydrate and lipid being oxidized. In rats, 48 h of starvation decreased the 13C/12C ratio of breath CO2 by 3.5‰. At this time the 13C/12C ratio of breath CO2 was very similar to that of plasma lipids. In humans, 30 min of heavy exercise increased the breath 13CO2/12CO2 ratio by 1.3‰. These changes in breath 13C/12C ratios could be predicted from 13C/12C ratios of plasma macronutrients and the percentage of carbon dioxide derived from each macronutrient, but only when compared within the same populations. For example, the 13C/12C ratios of plasma macronutrients of residents of Chicago, Illinois (USA) and Tokyo (Japan) differed by 1-3‰. An empirical correction of 13CO2 breath test data is recommended when breath tests are run under conditions that will change metabolic fuel utilization.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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