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  • 1
    ISSN: 1432-041X
    Keywords: aFGF ; bFGF ; Palate development ; Extracellular matrix ; Growth factors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The distribution of acidic and basic fibroblast growth factors (aFGF, bFGF) was mapped during mouse embryonic palate development. Generally, they localised most intensely in the basement membrane and epithelia rather than the mesenchyme. Localisation was predominantly restricted to the palatal nasal, and medial edge epithelia. Staining was particularly intense in the medial edge epithelia at the time of mid-line epithelial seam formation. Intense staining persisted in the epithelia of the degenerating seam and later in the oral and nasal epithelial triangles. Mouse embryonic palate mesenchyme (MEPM) cells cultured in vitro on a variety of substrata (on plastic, on the surface of a collagen gel and within a collagen gel) responded to treatment with aFGF or bFGF. These responses were modulated by the culture substratum. The FGFs stimulated MEPM cell proliferation on plastic and on collagen, but inhibited cell growth in collagen. The FGFs had little effect on protein production when cells were cultured on plastic, but caused a large reduction in on-collagen and incollagen cultures. This reduction was greater in collagenous than non-collagenous proteins. Generally, treatment with FGFs stimulated the production of glycosaminoglycans (GAGs), particularly hyaluronan (HA) and dermatan sulphate (DS). In addition, the size class of HA was shifted to a higher molecular weight form. These data indicate that aFGF and bFGF may play a role in modulating mesenchymal cell matrix biosynthesis, so facilitating palatal epithelial seam degeneration.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Surgical and radiologic anatomy 17 (1995), S. 1-5 
    ISSN: 1279-8517
    Keywords: Liver ; Biometry ; Perihepatic prosthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Sur une série de 50 pièces anatomiques d'adultes (27 hommes, 23 femmes), l'étude morphométrique du foie, de ses attaches dorsales et leurs variations a permis la confection d'une prothèse périhépatique en polyglactine adaptable à chaque lobe et fixée grâce à des artifices anatomiques. Celle-ci assure une compression efficace du parenchyme lésé et donc une hémostase et une bilistase correctes. Le ligament falciforme et le rétrecissement du parenchyme hépatique à son niveau assurent le maintien de la prothèse. Un exemplaire a été réalisé pour chaque lobe.
    Notes: Abstract We studied specimens from 50 cadavers (27 men and 23 women) to obtain anatomical data concerning the liver and its attachments. The results allowed us to develop a polyglactin perihepatic prosthesis for compression of the injured liver. The falciform ligament and a narrowing of the hepatic parenchyma at this level allow attachment of the prosthesis. Separate prostheses were designed for each lobe. Clinical use has demonstrated that our prosthesis can be used to achieve effective control of haemorrhage and bile leakage.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1279-8517
    Keywords: Larynx ; MRI ; Histology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The larynx is an organ with a complex anatomic structure. MRI allows the performance of sections in the three planes of space, so that this study of the soft parts of the larynx yields results superior to those of other imaging techniques. Together with laryngoscopy, MRI is most often used in assessing the extension of malignant laryngeal tumors. This assessment is fundamental in choosing the indications for surgery, but the published reports of MRI of the larynx are sometimes discordant. The visualization of certain important anatomic structures such as the conus elasticus is uncertain. Our aim was to study the MRI radio-anatomy of the larynx based on correlations between MRI and histologic sections. Eight anatomic specimens were studied four in the transverse plane, two in the sagittal plane, and two in the frontal plane. The MRI and histologic sections made at the same levels were compared. These comparisons allowed a description of the sectional radio-anatomy of the larynx and an assessment of the reliability and limitations of MRI. All the major anatomic structures could be identified. It was possible to demonstrate the conus elasticus. We were able to visualize the vocal process of the arytenoid cartilage, which has not to our knowledge been previously described in the literature.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1279-8517
    Keywords: Arteries ; Man ; Biomechanics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Dans le but d'étudier les propriétés mécaniques de la paroi artérielle et de pouvoir établir des comparaisons entre des segments artériels de structure histologique différente, nous avons mis au point un banc d'essai hydrodynamique permettant de tester des segments artériels dans des conditions voisines de la réalité physiologique. Un générateur hydrodynamique permettait d'obtenir dans un circuit ouvert un régime de pressions de type systolo-diastolique. Un capteur de pression intra-artériel, ainsi qu'un sonomicromètre relié à des cristaux piézo-électriques placés de façon diamétralement opposée sur la paroi artérielle, permettaient de calculer, pour un régime de pressions donné et grâce à l'acquisition de données dans un système informatique, la compliance, la rigidité, la contrainte trans-pariétale, le module de Young, le module incrémentiel d'un segment artériel. Nous avons étudié sept artères carotides communes (CC), et sept artères fémorales (superficielles) (F) fraîchement prélevées chez des sujets sains âgés de 18 à 35 ans. Des corrélations avec la richesse en fibres élastiques de la paroi artérielle ont été établies. Nous avons mis en évidence un phénomène d'hystérésis pour chaque artère testée quel que soit le niveau de pression considéré. La compliance des artères CC a été plus importante, les modules et la rigidité ont été moins importants et ce de façon significative par rapport aux artères F. Aucune altération histologique n'a été mise en évidence après passage des segments artériels au banc d'essai. Ces résultas préliminaires nous permettent d'envisager de nombreuses applications à ce travail dont l'une d'entre elles sera la mesure comparative des propriétés mécaniques des artères avant et après cryopréservation.
    Notes: Abstract In order to study the biomechanical properties of the arterial wall and to compare arteries with different histologic structures, we designed a device that allows testing of arterial segments under near-physiologic conditions. A hydrodynamic generator simulates systolo-diastolic pressures in an open loop. An intraarterial pressure sensor and a sonomicrometer connected to two piezoelectric crystals placed in diametric opposition on the arterial wall allow computer calculation of compliance, stiffness, midwall radial arterial stress, Young modulus, and incremental modulus for a given arterial segment at a given pressure setting. Seven healthy common carotid artery (CCA) segments and seven healthy (superficial) femoral artery (FA) segments were studied immediately after removal from brain-dead donors between the ages of 18 and 35 years. Histologic examination was performed to determine the density of elastic fibers in the arterial wall. Hysteresis was observed in all segments regardless of pressure settings. Compliance was greater and modulus values and stiffness were lower in CCA than in FA. No evidence of structural change was noted after testing in the circulation loop. These preliminary results open the way to a wide variety of applications for our hydrodynamic circulation loop. Experiments will be undertaken to compare the mechanical properties of arteries before and after cryopreservation.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Surgical and radiologic anatomy 6 (1984), S. 247-254 
    ISSN: 1279-8517
    Keywords: Greater splanchnic nerve ; Splanchnisectomy ; Transhiatus approach
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé La hauteur et le mode de constitution des nerfs grands splanchniques ainsi que leurs rapports dans le médiastin postérieur ont été étudiés pour préciser les bases anatomiques de l'abord trans-hiatal des nerfs grands splanchniques. Le système veineux azygos constitue le rapport essentiel. Le but de ce travail est de permettre une neurectomie bilatérale complète.
    Notes: Summary The level of origin and mode of constitution of the greater splanchnic nerve and its relations in the posterior mediastinum were studied. The aim of this work was to identify the anatomical basis of the transhiatus approach to the right and left greater splanchnic nerves. The azygos venous system was seen to be the main anatomical relation of these nerves. The results of this study should allow the surgeon to perform total bilateral neurotomy.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Surgical and radiologic anatomy 17 (1995), S. 161-169 
    ISSN: 1279-8517
    Keywords: Popliteal artery ; Popliteal entrapment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Nous rapportons 15 cas de syndrome de l'a. poplitée piégée observés chez 11 patients. Les causes anatomiques ont été les suivantes : dans un cas l'a. poplitée présentait un trajet aberrant en dedans du chef médial du m. gastrocnémien. Dans cinq cas existait une bandelette fibreuse reliant le chef medial du m. gastrocnémien au condyle latéral du fémur, et croisant l'a. poplitée par en arrière ; dans cinq cas cette anomalie était également présente associée à une insertion anormalement haute et/ou médiale du chef médial du m. gastrocnémien. Dans les quatre derniers cas existait une anomalie d'insertion associée à une hypertrophie musculaire responsable d'une compression artérielle. L'artériographie pratiquée chez les 11 patients a montré des signes évocateurs du diagnostic dans tous les cas où l'artère était perméable. Deux aa. poplitées étaient occluses. L'examen tomodensitométrique et l'IRM des fosses poplitées ont permis de préciser l'origine musculaire de la compression poplitée. Tous les patients ont été opérés ; deux ont bénéficié d'un pontage en v. grande saphène inversée et tous les autres ont été traités par libération de l'a. et/ou de la v. poplitée par voie postérieure. Les suites opératoires ont été simples chez tous les patients y compris à long terme. Tous les patients ont pu reprendre leur activité physique sans séquelle. Notre revue de la littérature qui porte sur 374 pièges poplités observés chez 280 patients permet de préciser la fréquence des différentes anomalies observées, la symptomatologie qu'elles entraînent ainsi que les différentes possibilités thérapeutiques. Les multiples classifications anatomiques ainsi que l'embryologie artérielle et musculaire sont également rappelées.
    Notes: Summary We report 15 examples of popliteal artery entrapment syndrome observed in 11 patients. The anatomical causes were as follows: in one case, the popliteal artery presented an aberrant course medially to the medial head of the gastrocnemius muscle. In 5 cases, there was a small fibrous band linking the medial head of the gastrocnemius muscle to the lateral condyle and crossing behind the popliteal artery; in 5 cases this anomaly was also found in association with an abnormally high and/or internal insertion of the medial head of gastrocnemius muscle. In the last 4 cases, there was a muscular insertion anomaly associated with muscular hypertrophy causing arterial compression. Arteriography performed in the 11 patients showed evocative signs of the diagnosis in all cases where the artery was patent. Two popliteal arteries were occluded. CT scan and MRI examination of the popliteal fossa enabled us to define the muscular origin of the popliteal compression. All of the patients were operated upon; two received a reversed saphenous bypass and all of the others were treated by liberation of the popliteal artery and/or vein by a posterior approach. Follow-up in all patients at long term showed good prognosis. All of the patients were able to take up their previous physical activities without sequelae. Our review of the literature, which is based on 374 cases of popliteal artery entrapment observed in 280 patients, made it possible to define the frequency of the various anomalies observed, their symptoms and the different therapeutic possibilities. The multiple anatomical classifications as well as the arterial and muscular embryology are also described.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-1041
    Keywords: methotrexate ; osteosarcoma ; high parenteral dose ; pharmacokinetics ; drug monitoring ; computer prediction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary Methotrexate (MTX) in serum was measured by RIA in 12 cancer patients receiving high doses of MTX (2 to 8 g/m2) in 6 hour infusions 69 treatments were studied. The peak serum level was proportional to the dose administered and was always greater than 10−4 M. 2 elimination phases were seen: the first had a mean half-life of 2.36 h and the second a mean half-life of 16.14 h. 24 hours after beginning the infusions there were very large variations in individual serum concentrations of MTX, from 2.4 10−6 M to 1.9 10−5 M by 24 h after 8 g/m2. To control these variations, a mathematical model for prediction of the individual pharmacokinetic pattern of a 6 hour-infusion of high-dose MTX by kinetic analysis of a low-test dose is proposed. A program was created for an Apple III computer using toxic and therapeutic serum levels of MTX selected by the clinician. The computer program is adaptable to any infused substance for variable infusion times, thus introducing new advances over existing methods.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-1041
    Keywords: Key words Schizophrenia ; Haloperidol; radio-receptor assay ; dopaminergic receptor ; clinical evaluation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Abstract Objective: We report preliminary results from use of a radioreceptor assay (RRA) to measure serum haloperidol levels and their relation to clinical response and adverse effects in 19 psychotic patients with positive symptoms treated with haloperidol. Methods: Blood samples were taken on Days 7, 14 and 21. Clinical evaluation was based on the PANSS, and UKU scales. The D2 antidopaminergic RRA was used to measure haloperidol serum levels. Results: Our results show a correlation between psychosis with positive symptoms assessed by positive scores in the PANSS and the serum drug level measured by RRA concentrations. The observed relationship made it possible to establish a therapeutic serum range of haloperidol equivalents 15–30 ng/ml−1. This range contained no “non-responders”. Analysis of the correlation between each item on the positive subscale of the PANSS and the RRA concentration suggested that there was no specific symptom of psychosis closely related to RRA concentration during the first 15 days of treatment. In the third week, however, certain specific symptoms were closely connected to the RRA concentration. Conclusion: From these results, it can be concluded that antipsychotic activity can be related to blockade of serum D2 dopamine receptors. In future would be desirable to monitor neuroleptic treatment in this way. The study also showed that the antipsychotic treatment did not correct any specific component of the psychosis during the first stage of the treatment but that it did so at later times.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1279-8509
    Keywords: Lymphoblasts ; Lymphoblastic ; Leukemias and lymphomas ; Adhesion molecules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We report two cases of subcutaneous lymphomas with a study of cellular adhesion molecules compared to those expressed on blasts at a later blastic phase. The striking different profile of adhesive phenotype in the two conditions is the expression of the interactions of blasts with their microenvironments. It seems that β1 integrins-dependant pathway sustains mainly the development of hematopoietic precursors in extra-medullary niches, while their contact with bone-marrow stroma and their trans-endothelial migration implicates Selectins and Immunoglobulins molecules.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Carbohydrate Research 190 (1989), S. C7-C10 
    ISSN: 0008-6215
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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