ISSN:
1432-072X
Keywords:
Key wordsAcidaminococcus fermentans
;
Trans-
;
aconitate fermentation
;
Hydrogen formation
;
Na+-dependence
;
Citrate Si-lyase
;
Mg2+-dependent
;
oxaloacetate decarboxylase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Growing cells of Acidaminococcus fermentans (DSM 20731 and ATCC 25085) fermented trans-aconitate via citrate, oxaloacetate, and pyruvate to approximately 2 CO2, 1.8 acetate, 0.1 butyrate and 0.9 H2. The carbon and electron recoveries were close to 100%. On citrate no growth was observed and washed cells were unable to ferment this tricarboxylate. In cell-free extracts, however, citrate as well as trans-aconitate were readily fermented to CO2 and acetate. Under these conditions, also cis-aconitate, oxaloacetate, and pyruvate were formed, whereas butyrate and intermediates of glutamate fermentation, 2-oxoglutatrate and glutaconate, could not be detected. Citrate Si-lyase, a Mg2+-dependent oxaloacetate decarboxylase, and pyruvate synthase were present in quantities that corresponded to the growth rate of the organism.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002030050393
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