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  • 1
    Electronic Resource
    Electronic Resource
    Effects of soil invertebrates on the survival of some genetically engineered bacteria in leaf litter and soil (1996)
    Springer
    Biology and fertility of soils 23 (1996), S. 221-228 
    Details
    ISSN: 1432-0789
    Keywords: Key words Genetically engineered bacteria ; Soil invertebrates ; Leaf litter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Seven bacterial strains, most of them bearing natural or recombinant plasmids, were introduced in oak leaf litter or soddy-podzolic soil. In these substrata, which contained litter-dwelling diplopods and isopods, or endogenic earthworms, bacteria survival was followed. In the absence of the animals, the numbers of introduced strains gradually decreased. In the presence of the animals, plasmid-bearing strains of Pseudomonas putida survived at 105–107 CFUs g–1 up to 1.5 months in both leaves and soil. The total numbers of bacteria found in excrements from the soil macrofauna were 5–15 times higher than in the food. The numbers of P. putida in the excrements were equal to or higher than in the food. The numbers of Pseudomonas stutzeri JM302 (pLV1013) and Azospirillum brasiliense ATCC29710 (pFACII) in the excrements were always 2–10 times lower than in the food. The digestive fluid taken from the middle part of the gut of the diplopod Pachyiulus flavipes showed a strong antibacterial activity. Those bacteria with lower survival in the gut appeared to be more sensitive to digestion by the midgut fluid. In contrast, the hindgut fluid did not suppress the viability of P. stutzeri JM302 (pLV1013). We postulate that the introduced bacteria partially survive the midgut passage and then multiply with a high growth rate in the hindgut of the animals. The environmental consequences of the interactions between soil invertebrates and the released bacteria are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00335948
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Yeasts associated with soil invertebrates (1993)
    Springer
    Biology and fertility of soils 16 (1993), S. 183-187 
    Details
    ISSN: 1432-0789
    Keywords: Yeasts ; Soil invertebrates ; Gut microbiota ; Symbiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The hindgut of the diplopod Pachyiulus flavipes is inhabited by specific yeast populations. The yeast communities consist almost exclusively of ascomycetes, viz., Debaryomyces hansenii, Torulaspora delbrueckii, and Zygowilliopsis californica. Evidence is provided that the yeasts isolated from the gut can be considered symbionts that remain in a steady state under different feeding and rearing conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00361405
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Plasmid transfer between introduced and indigenous bacteria in leaf litter, soil and vermicompost as affected by soil invertebrates (1999)
    Springer
    Biology and fertility of soils 28 (1999), S. 169-176 
    Details
    ISSN: 1432-0789
    Keywords: Key words Genetically modified bacteria ; Soil invertebrates ; Plasmid transfer ; Pseudomonas spp. ; Enterobacter cloacae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  Plasmid transfer between introduced strains of Pseudomonas stutzeri JM302 (pLV1013), Pseudomonas putida PAW340 (pLV1017), Pseudomonas aeruginosa PAO5 (RP4), and Enterobacter cloacae MF10 (RP4), all of them harbouring genetically modified or naturally occurring plasmids and bacteria indigenous to oak leaf litter, soddy-podzolic soil or vermicompost was monitored using non-sterile laboratory microcosms inhabited by the millipede Pachyiulus flavipes, the woodlouse Armadillidium vulgare, or the earthworms Aporrectodea caliginosa and Eisenia fetida, respectively. Plasmid transfer from genetically modified Streptococcus faecalis JH2-2 (pAMβ1) to Bacillus thuringiensis var. israelensis 1-5 under similar conditions was also followed. A recombinant conjugative plasmid (pLV1017) encoding resistance to antibiotics and expressing catechol 2,3-dioxygenase was transferred into two facultatively anaerobic gram-negative bacteria, isolated from the excrement of A. vulgare. Presumed transconjugants of the facultatively anerobic gram-negative bacteria given above were isolated from the leaves and soil (four strains) and the excrement of the invertebrates (11 strains). They carried the same plasmids and exhibited the same enzymatic activity as the respective inoculant strains; for most of them, however, DNA fingerprints were slightly different. Transfer from S. faecalis JH2-2 to B. thuringiensis var. israelensis of a naturally occurring plasmid, pAMb1, encoding resistance to erythromycin was observed in vermicompost.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003740050480
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Effects of soil invertebrates on the survival of some genetically engineered bacteria in leaf litter and soil (1996)
    Springer
    Biology and fertility of soils 23 (1996), S. 221-228 
    Details
    ISSN: 1432-0789
    Keywords: Genetically engineered bacteria ; Soil invertebrates ; Leaf litter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Seven bacterial strains, most of them bearing natural or recombinant plasmids, were introduced in oak leaf litter or soddy-podzolic soil. In these substrata, which contained litter-dwelling diplopods and isopods, or endogenic earthworms, bacteria survival was followed. In the absence of the animals, the numbers of introduced strains gradually decreased. In the presence of the animals, plasmid-bearing strains of Pseudomonas putida survived at 105–107 CFUs g-1 up to 1.5 months in both leaves and soil. The total numbers of bacteria found in excrements from the soil macrofauna were 5–15 times higher than in the food. The numbers of P. putida in the excrements were equal to or higher than in the food. The numbers of Pseudomonas stutzeri JM302 (pLV1013) and Azospirillum brasiliense ATCC29710 (pFACII) in the excrements were always 2–10 times lower than in the food. The digestive fluid taken from the middle part of the gut of the diplopod Pachyiulus flavipes showed a strong antibacterial activity. Those bacteria with lower survival in the gut appeared to be more sensitive to digestion by the midgut fluid. In contrast, the hindgut fluid did not suppress the viability of P. stutzeri JM302 (pLV1013). We postulate that the introduced bacteria partially survive the midgut passage and then multiply with a high growth rate in the hindgut of the animals. The environmental consequences of the interactions between soil invertebrates and the released bacteria are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00335948
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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