Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Extracellular glycosaminoglycan changes in healthy and overgrown gingiva fibroblasts after cyclosporin A and cytokine treatments (2004)
    Oxford, UK : Munksgaard International Publishers
    Journal of oral pathology & medicine 33 (2004), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background:  It has been demonstrated that cyclosporin A (CyA) blocks the immune system, acts on cytoskeleton and stimulates the production of extracellular matrix (ECM) and transforming growth factor-β1 (TGF-β1). This cytokine, such as transforming growth factor-α (TGF-α), induces deposition of glycosaminoglycans (GAG), proteoglycans and collagen fibres in the ECM.Methods:  In this work, we examined the effect induced by CyA, TGF-β1 and TGF-α on cultures of healthy and overgrown human gingival fibroblasts in order to evaluate the glycosaminoglycan, cytoskeletal changes and the behaviour of fibroblasts after concanavalin A (Con A) treatment. Moreover, we examined gingival biopsies by Alcian blue histochemical staining and electron transmission microscopy.Results:  Total and extracellular sulphated GAG in overgrown gingiva specimens and in derived fibroblast cultures treated with CyA and cytokines were significantly higher than controls. The action of cytokines was increased (P ≤ 0.01) compared with CyA with a greater effect of TGF-α in comparison with TGF-β1; the electron microscopy showed ECM accumulation. The agglutinations showed the heterogeneity of fibroblast populations.Conclusions:  Stimulation with Con A showed that the fibroblast population had cell surface heterogeneity, and could respond in a different way to both CyA and cytokine stimulus. Moreover, increased synthesis of GAG in overgrown gingiva compared with synthesis in normal fibroblasts before CyA treatment suggests a possible genetic origin of damage. As not all CyA-treated patients develop gingival overgrowth, a genetic predisposition may explain the different responses of gingival fibroblast populations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.2004.00217.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Influence of progesterone on protein and RNA synthesis in cultured chick embryo liver cells (1976)
    Springer
    Cellular and molecular life sciences 32 (1976), S. 42-43 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Chick embryo liver primary cultures, when added with progesterone, exhibit, in comparison with the controls, a normal growth, a decline of both3H-uridine uptake and incorporation into total RNA, a decline of3H-leucine and14C serine incorporation into the proteins. Progesterone is not able to stimulate phosvitin synthesis induction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01932613
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Estrogen-induced ultrastructural changes in cultured chick embryo hepatocytes (1979)
    Springer
    Cell & tissue research 199 (1979), S. 249-256 
    Details
    ISSN: 1432-0878
    Keywords: Hepatocytes ; Birds ; Ultrastructure ; Estrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Administration of estradiol-17-β induces the synthesis of vitellogenin in primary cultures of chick embryo liver. The ultrastructural changes accompanying steroid induced vitellogenin synthesis were investigated in hepatocytes cultures incubated for 0, 12 and 24 h following hormonal treatment. Both electron microscopy and immuno-chemical techniques were used. The immuno-fluorescence data indicate that the cultures contain a cell population of 90–95% hepatocytes, both in control and estradiol-treated groups. Ultrastructurally, cultured hepatocytes are similar to in vivo ones, except for a reduced accumulation of storage materials, glycogen and lipids. In the estradiol-treated cultures hepatocytes show an accumulation of free ribosomes, enlargement of rough endoplasmic reticulum, and development of the Golgi apparatus. The data are discussed in relation to previous biochemical findings.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236136
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...