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  • 1
    Electronic Resource
    Electronic Resource
    Expression of neurotrophins, GDNF, and their receptors in rat thyroid tissue (1999)
    Springer
    Cell & tissue research 295 (1999), S. 467-475 
    Details
    ISSN: 1432-0878
    Keywords: Key words Glial cell line-derived neurotrophic factor ; GDNF ; Ret ; GDNFR-α ; Brain-derived neurotrophic factor ; BDNF ; NT-3 ; NT-4 ; trk receptors ; Thyroid tissue ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Levels of mRNA for neurotrophins (brain-derived neurotrophic factor, BDNF; neurotrophin 3, NT-3; neurotrophin 4, NT-4) and their receptors (trkA, trkB, trkC) and for glial cell line-derived neurotrophic factor (GDNF) and its receptors (ret, GDNFR-α) were measured in rat thyroid tissue by ribonuclease protection assays. In thyroid tissue the NT-3 mRNA level was threefold lower and the NT-4 mRNA level sixfold higher than those detected in adult rat hippocampus, while BDNF mRNA was undetectable. Very low levels of mRNA for truncated trkB and trkC receptors and no catalytic trkA, trkB or trkC were found. In conclusion NT-3 and NT-4, but not the corresponding functional receptors, are expressed in the thyroid tissue. Therefore, it is unlikely that these factors serve a direct local autocrine or paracrine function in thyroid cell types, and a target-derived mode of action on neurons innervating the thyroid tissue is suggested. An opposite result has been found for the neurotrophic factor GDNF: thyroid tissue showed a high level of transcripts for the GDNF receptor subunits (GDNFR-α and Ret), while GDNF mRNA was undetectable. The in situ hybridization analysis of GDNFR-α and ret mRNA revealed an interesting difference in the cell distribution of these transcripts: ret mRNA is selectively expressed in a subpopulation of cells scattered in the follicular epithelium and in the interfollicular spaces, while GDNFR-α expression is more homogeneous and widespread, including the more abundant cell type of the thyroid gland: the follicular cell. Double-labeling in situ hybridization/immunocytochemistry experiments, with a specific marker (calcitonin), showed that parafollicular cells express ret but not GDNFR-α. This differential distribution of the GDNF receptor components (GDNFR-α and ret) may reflect a peculiar biological role in intercellular communication in the thyroid gland.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410051252
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  • 2
    Electronic Resource
    Electronic Resource
    EDXRS study of lichens as biomonitors and effects of washing procedure on element concentrationsPresented at the European Conference on EDXRS, Budapest, Hungary, 30 May-3 June 1994. (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    X-Ray Spectrometry 24 (1995), S. 143-146 
    Details
    ISSN: 0049-8246
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: A monitoring survey was carried out in North Italy using Pseudevernia furfuracea as a monitor of air pollution. The experiment was carried out on lichen thalli exposed for different periods varying from 3 to 10 months at urban and rural sites, to examine and compare the bioaccumultion of several elements. Every 2 months a fraction fo exposed lichens was collected to analyse the theallus. Each fraction was divided into two parts, one of which was washed in deionized water, in order to quanify the element loss due to the washing procedure. The samples were analysed by energy-dispersive x-ray fluorescence spectrmetry in the secondary excitation mode; quantitative analysis was performed by the fundamental parameter method with NIST standard reference materials. The targets for the fluorescence spectrometer were obtained by digestion under pressure with microwave heating. At the end of the exposure period (10 months), nearly all elements, showed highter concentrations at the urban than at the rural site.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/xrs.1300240311
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    Paper (German National Licenses)
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