ISSN:
1432-1106
Keywords:
Heat shock protein
;
Bicuculline
;
Convulsion
;
Electroencephalography
;
Immunoblotting
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Exposure to elevated temperature provokes a sequence of events (heat shock response) in all living organisms. Through this response, heat shock proteins (HSPs) are induced and protect the cells against subsequent injury. We investigated the effect of heat treatment on bicuculline-induced convulsions, and analyzed a possible role of HSPs. Screw electrodes were implanted in the brain of mature male Wistar rats for electroencephalogram (EEG) recording. Experimental rats were subjected to whole-body hyperthermia at 41–42° C for 15 min. Fifteen hours later, bicuculline was injected intra peritoneally to induce convulsions in both experimental and control groups. The heated rats showed a significant attenuation of the convulsive response, in terms of both spike discharges in EEG and clinical seizures. Furthermore, induction of HSP72 was detected in the brain of heat-treated rats by immunoblotting, appearing at 4 h and reaching a maximal level 16–24 h after the heat shock. We conclude that the previous heat treatment stabilized neuronal excitability, most probably through the induction of HSP72.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00242900
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