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  • 1
    Electronic Resource
    Electronic Resource
    Variation in genome organization of the plant pathogenic fungus Colletotrichum lindemuthianum (1998)
    Springer
    Current genetics 33 (1998), S. 291-298 
    Details
    ISSN: 1432-0983
    Keywords: Key wordsColletotrichum lindemuthianum ; Genome plasticity ; Chromosome ; Telomere
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The genome structure of Colletotrichum lindemuthianum in a set of diverse isolates was investigated using a combination of physical and molecular approaches. Flow cytometric measurement of genome size revealed significant variation between strains, with the smallest genome representing 59% of the largest. Southern-blot profiles of a cloned fungal telomere revealed a total chromosome number varying from 9 to 12. Chromosome separations using pulsed-field gel electrophoresis (PFGE) showed that these chromosomes belong to two distinct size classes: a variable number of small (〈 2.5 Mb) polymorphic chromosomes and a set of unresolved chromosomes larger than 7 Mb. Two dispersed repeat elements were shown to cluster on distinct polymorphic minichromosomes. Single-copy flanking sequences from these repeat-containing clones specifically marked distinct small chromosomes. These markers were absent in some strains, indicating that part of the observed variability in genome organization may be explained by the presence or absence, in a given strain, of dispensable genomic regions and/or chromosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050339
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of temperature on head blight of wheat caused by Fusarium culmorum and F. graminearum (2005)
    Oxford, UK : Blackwell Science Ltd
    Plant pathology 54 (2005), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The effect of small temperature differentials (16 vs. 20°C) on the pathogenicity of deoxynivalenol producing single isolates of Fusarium culmorum and F. graminearum and on the fusarium head blight (FHB) response of eight wheat cultivars was examined. Fusarium culmorum inoculation caused greater visual disease symptoms at 20°C than at 16°C, both overall and on an individual cultivar basis (overall AUDPC = 13·5 and 9·6, respectively) (P 〈 0·05). In contrast, F. graminearum inoculation caused greater overall visual disease symptoms at 16°C than at 20°C, both overall and at the individual cultivar level (overall AUDPC = 12·8 and 10·9, respectively) (P 〈 0·05). Results showed both F. culmorum and F. graminearum inoculations caused a greater loss in yield at 20°C (54·3 and 46·9% relative 1000-grain weight, respectively) compared with 16°C (73·3 and 66·9% relative 1000-grain weight, respectively) (P 〈 0·05). Fusarium culmorum-inoculated heads contained similar amounts of fungal DNA at both 16 and 20°C (1·9 and 1·7 ng mg−1 of plant material, respectively) (not significant), while for F. graminearum inoculation, plants contained higher amounts of fungal DNA at 20°C (2·0 and 1·0 ng mg−1 of plant material, respectively) (P 〈 0·05). Overall, there was a significant negative correlation between AUDPC and percentage relative 1000-grain weight at both 16 and 20°C (r =−0·693 and −0·794, respectively, P 〈 0·01).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3059.2005.01157.x
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  • 3
    Electronic Resource
    Electronic Resource
    Response of wheat and barley isolates of Septoria nodorum to passage through barley and wheat cultivars (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 31 (1982), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A single-spore wheat isolate of S. nodorum was passaged once through barley and a barley isolate passaged once through wheat. Reisolations from barley leaves were designated as either ‘original-type wheat’ (OTW) and resembled the wheat isolate used for inoculations in morphology, or ‘sector-type barley’ (STB) resembling the known morphology of barley isolates. Reisolations from wheat were similarly designated either OTB or STW. Sub-cultures of STB were significantly more pathogenic to barley in a glasshouse experiment than OTW, and vice versa. Similarly STW was significantly more pathogenic to wheat than OTB, and vice versa.A single-spore wheat isolate was also passaged 0, 1, 2, 3 or 4 times through barley and a barley isolate passaged similarly through wheat. Disease assessment and yield data from a field experiment showed that the pathogenicity of the passaged wheat isolate remained largely unaltered from that of the original isolate. However, the barley isolate responded after the third wheat passage by causing significantly less disease and significantly higher yields in barley than the unpassaged isolate; conversely, on wheat, disease levels significantly increased and yield slgnficantly decreased after the second wheat passage.Data obtained are discussed in the context of cultural observations made on colony morphology during host passage sequences and possible genetic mechanisms responsible.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3059.1982.tb01284.x
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  • 4
    Electronic Resource
    Electronic Resource
    A Field Inoculation Method for Septoria tritici and Septoria nodorum (1970)
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 19 (1970), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A method is described, using a Czapek Dox V-8 agar and continuous irradiation with near-ultraviolet light, which enables large numbers of spores of Septoria tritici and Septoria nodorum to be produced rapidly and also allows easy identification of the two pathogens by their colony morphology. Also, a simple field inoculation method is described which has proved successful both in experiments involving different inoculum concentrations and for the screening of spring and winter wheat cultivars for their reactions against both pathogens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3059.1970.tb00983.x
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  • 5
    Electronic Resource
    Electronic Resource
    Studies on dispersal of Septoria tritici pycnidiospores in wheat–clover intercrops (1998)
    Oxford, U.K. and Cambridge, USA : Blackwell Publishers
    Plant pathology 47 (1998), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Four experiments studied the effects of a clover understorey on pycnidiospore dispersal of Septoria tritici in a wheat–clover intercrop under simulated rain. Clover significantly reduced the dispersal of spores in a horizontal direction by 33% at a distance of 15 cm from a line inoculum source compared with a wheat monocrop. The clover also reduced the vertical movement of spores from infected leaves at the base of wheat plants by an average of 63% compared to the monocrop, and this suggests that the main movement of spores was from the base upwards. Splash experiments using blue colour marker showed the vertical decline of splash and the number of drops per cm2 with height caught on paper adjacent to trays of clover was described by the exponential decline model. The effect of clover in reducing vertical splash approached an asymptote as the leaf area index of the understorey increased. Simulated rain-splash increased the level of disease on the flag leaf and, in one experiment, there was a significant interaction between rain-splash and clover in reducing the number of lesions on the flag leaf. The level of disease resulting from one splash event was low, indicating that subsequent pathogen multiplication is probably required to bring about high severities of disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3059.1998.00200.x
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  • 6
    Electronic Resource
    Electronic Resource
    NEW OR UNUSUAL RECORDS: A seedling blight of barley caused by Septoria nodorum (1983)
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 32 (1983), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3059.1983.tb01322.x
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