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  • 1
    Electronic Resource
    Electronic Resource
    Escherichia coli formate dehydrogenase mutants with altered selenopolymer profiles (1989)
    Springer
    Archives of microbiology 152 (1989), S. 397-400 
    Details
    ISSN: 1432-072X
    Keywords: Escherichia coli mutants ; Formate dehydrogenase ; Selenopolymers ; Malachite green
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Four classes of Escherichia coli mutants deficient in either or both of their anaerobic selenium-containing formate dehydrogenases (FDH) were isolated. A class I mutant devoid of FDHH activity specifically linked to benzyl viologen (BV) produced a small amount of the FDHH 80,000 dalton selenopeptide. Three class II mutants were deficient in FDHN activity specifically linked to phenazine methosulfate (PMS) and exhibited a selenopeptide “doublet” rather than the FDHN 110,000 dalton selenosubunit. Three class III mutants were selenium incorporation deficient and did not exhibit either FDH activity or 75Selabeled selenopolymers. A class IV mutant was devoid of PMS-linked FDHN activity; neither its FDHN 110,000 dalton selenosubunit nor its BV-linked FDHH activity was fully regulated by nitrate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425180
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    An immunofluorescence study of influenza virus filament formation (1980)
    Springer
    Archives of virology 63 (1980), S. 275-284 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A study is described in which filamentous forms of influenza virus were observed budding from host cell surfaces. Cell cultures infected with influenza virus were stained by indirect immunofluorescence using an antiserum to purified haemagglutinin. Filaments greater than 100 µm in length, with several branch points along their length were observed; the number and length of the filaments varied according to the virus strain and the time after infection. Examination of infected cells by electron microscopy confirmed the presence of branched structures with an ultrastructure typical of filamentous forms of influenza virus. The immunofluorescence technique was quicker than thin section electron microscopy and was a more sensitive procedure for the detection of filamentous forms of influenza virus than electron microscopy using negative stain. It also enabled the antigenic composition of the filaments to be observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01315033
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The ultrastructure ofEncephalitozoon cuniculi growing in renal tubules of rabbits (1981)
    Springer
    Parasitology research 64 (1981), S. 271-278 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A wild type rabbit was infected orally with cell culture-grownEncephalitozoon cuniculi. Twelve weeks after infection the rabbit was killed and blocks of kidney tissue were fixed for histology and electron microscopy.E. cuniculi were observed within kidney collecting tubule cells. The ultrastructure and development ofE. cuniculi in these cells was similar to that described in cultured cells and peritoneal macrophages.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00927374
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    The infectivity ofEncephalitozoon cuniculi in vivo and in vitro (1986)
    Springer
    Parasitology research 72 (1986), S. 65-72 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The infectivity ofEncephalitozoon cuniculi grown in cell cultures was determined in cultured cells and in wild and domestic rabbits. The ratio of the total to tissue culture viable count was 1,300 (median of seven determinations). The mean ratio of intact spore count to total count, as determined by electron microscopy was 0.12. Although variation between infectivity experiments was large, the median animal infective dose contained 51 FFU (cell culture focus-forming units) for wild rabbits (Oryctolagus cuniculus) and 40 FFU for domestic rabbits. These two infectivities were not statistically different.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00927735
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    Paper (German National Licenses)
    Fulltext
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