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  • 1
    Electronic Resource
    Electronic Resource
    Membrene turnover in imbibed and dormant embryos of the wild oat (Avena fatua L.) (1978)
    Springer
    Planta 139 (1978), S. 209-217 
    Details
    ISSN: 1432-2048
    Keywords: Avena ; Dormancy (embryos) ; Embryos ; Membranes ; Protein-turnover ; Viability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A comparative study of protein synthesis has been carried out with embryos excised from dormant (D) and non-dormant (ND) caryopses of the wild oat. Although D embryos imbibed in water or ND embryos imbibed in abscisic acid do not germinate, they incorporate [14C]leucine into TCA-insoluble material for the first 48 h as readily as embryos that do germinate (ND embryos imbibed in water, or D embryos imbibed in gibberellic acid). Pulsechase experiments with [14]leucine show that in both D and ND embryos the proteins associated with the membranes undergo turnover. The rates of decay of incorporated radioactivity are similar in both dormant and germinating embryos up to 98 h following embryo excision. Fractionation of the membrane proteins in SDS-polyacrylamide gels indicates that the different polypeptides have different rates of turnover. It is concluded that membrane proteins in imbibed D embryos are in a state of constant turnover, and that this is a part of the replacement processes necessary to maintain the integrity of hydrated cells. The continuation of such synthetic events could account for long term survival of dormant Avena fatua in the imbibed state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388632
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Membrane turnover in imbibed dormant embryos of the wild oat (Avena fatua L.) (1978)
    Springer
    Planta 139 (1978), S. 219-226 
    Details
    ISSN: 1432-2048
    Keywords: Avena ; Dormancy (embryos) ; Embryos ; Membranes ; Phospholipids ; Protein-turnover ; Viability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Germinating non-dormant (ND) embryos of wild oat incorporate [3H]glycerol into phospholipid, and a 250% increase in total extractable phospholipid occurs within 72 h. During germination, leveles of phosphatidyl inositol showed the greatest change, increasing approximately 5-fold. Imbibed dormant (D) embryos of the wild oat also incorporate [3H]gycerol into phospholipids, but there is no net synthesis. A continuous turnover of membrane phospholipids could be demonstrated in pulse chase experiments, and although the proportions of most phospholipids does not change, there was a decrease of 50% in phosphatidyl serine. The half-life of [3H]glycerol in the extracted phospholipids of D and ND embryos varies between 35 and 57 h, and in membrane fractions separated on sucrose density gradients the half-lives vary between 26 and 56 h. D embryos induced to germinate with GA and ND embryos in which germination is repressed by ABA show similar phospholipid changes to ND and D embryos respectively, with the exception that the proportion of phosphatidyl serine remained unchanged in the ND-ABA embryos. It is concluded that the continual turnover of membranes of imbibed dormant embryos is consistent with the maintenance of cellular integrity determining the longevity of the seed under natural conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388633
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Molecular cloning and chromosomal location of genes encoding the “Early-methionine-labelled” (Em) polypeptide of Triticum aestivum L. var. Chinese Spring (1990)
    Springer
    Theoretical and applied genetics 80 (1990), S. 43-48 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Em genes ; Group 1 chromosomes ; RFLPs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The “Early-methionine-labelled” (Em) polypeptide is the most abundant cytosolic polypeptide found in mature wheat embryos. Using a near full-length cDNA clone as a hybridisation probe to detect genomic sequences by Southern blotting of electrophoretic separations of genomic DNA derived from Triticum aestivum L. var. Chinese Spring and a series of its aneuploid derivatives, we demonstrate that the Em polypeptide is the product of a small multigene family in which the copies are located on each of the long arms of the homoeologous group 1 chromosomes. Screening of a variety of genotypes additionally reveals a number of restriction fragment length polymorphisms associated with these loci. Screening of a library of genomic DNA cloned in the vector λEMBL 4 has resulted in the isolation of a genomic fragment containing two closely linked Em genes. These are separated by ca. 2.5 kb. Analysis of restriction enzyme digests of this clones fragment has identified it as originating from chromosome 1A.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224014
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    Paper (German National Licenses)
    Fulltext
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