ISSN:
1617-4623
Keywords:
Bryophyta
;
Bud-induction (moss)
;
Cytokinin over-producing mutant
;
Physcomitrella patens
;
Temperature-sensitive mutant (moss)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A mutant of the moss, Physcomitrella patens, was isolated which was temperature-sensitive for the production of gametophores. At 17° C this mutant, designated ove 409, produced normal leafy shoots. At 24° C ove 409 produced many abnormal buds characteristic of bud-over-producing (ove) mutants. ove 409 produced an intermediate phenotype at 21° C. The cytokinin levels in the culture medium of this mutant, the wild-type and a cytokinin overproducing mutant, oveA78, were measured by combined gas chromatography mass spectrometry at the permissive and nonpermissive temperatures. Production of cytokinin was found to be affected by temperature in all strains; the change in phenotype of ove 409 correlated with the production of N6-(Δ2-isopentenyl) adenine. Complementation analysis was performed using this mutant by protoplast fusion. ove 409 was found to be in the same complementation group as a previously isolated ove mutant, oveA78.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00422081
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