ISSN:
0930-7516
Keywords:
Chemistry
;
Industrial Chemistry and Chemical Engineering
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
The efficiency of 6-aminopenicillanic acid (6-APA) production by enzyme-catalyzed hydrolysis of penicillin G using penicillin G amidase (E. C. 3.5.1.11) immobilized in polyacrylamide gel can be improved by using a lower substrate concentration and an optimized low buffer concentration. In a continuous process the combination of a stirred-tank reactor and a plug-flow reactor gives optimum productivity. Since precipitation of 6-APA is the crucial step in downstream processing, the product concentration has to be as high as possible. This can be achieved by concentrating the product solution by reverse osmosis.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/ceat.270180406
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