ISSN:
1432-0991
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract TheE. coli rnc gene encodes the double-stranded, RNA-specific ribonuclease III (RNaseIII). A novel bacteriophage, gy1, was isolated, and its propagation inE. coli was shown to depend on the expression of RNaseIII in the cell. (a) gyl has a low efficiency of plating on rnc+ strains and a high efficiency of plating on a rnc− E. coli strain harboring the rnc 105 point mutation that renders its RNaseIII product inactive. (b) gy1 has a high efficiency of plating on rnc− strains in which thernc gene is disrupted by a ΔTn10 insertion. (c) Plasmids harboring a rnc+ gene that were introduced into the rnc− strains described above reduced the efficiency of plating of gy1.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01570899
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