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  • 1
    Electronic Resource
    Electronic Resource
    Effect of various root surface treatments on the attachment and growth of human gingival fibroblasts: histologic and scanning electron microscopic evaluation (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of clinical periodontology 11 (1984), S. 0 
    Details
    ISSN: 1600-051X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract Human teeth extracted because of advanced periodontal disease were obtained. The portions of the roots which had been exposed in periodontal pockets were either untreated or were treated with root planing or citric acid, or root planing followed by citric acid. Human gingival fibroblasts were then added to the roots so treated and were allowed to incubate for 72 h. The ability of cells to attach to and grow onto these roots was assessed by means of gross evaluation of staining intensity and by histologic and scanning electron microscopic observation. The results of multiple experiments in each root-treatment category indicated that only roots which had been planed, whether or not citric acid demineralization was used, promoted cell attachment and growth. In addition, there were no discernible morphologic differences in the cells which were plated onto roots which were root planed only, compared to those which were root planed and citric-acid treated. In both situations too, the cells displayed morphology typical of human gingival fibroblasts in culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-051X.1984.tb00905.x
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  • 2
    Electronic Resource
    Electronic Resource
    Ultrastructural features of the verruciform xanthoma (1976)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral pathology & medicine 5 (1976), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The verruciform xanthoma, a rare lesion of the oral cavity, was studied by light and electron microscopy. The major cell type associated with the lesion was shown to contain appreciable amounts of lipid and was characterized as a macrophage. It was characteristic of the endothelial cells associated with subepithelial capillaries to exhibit multiple basal laminae. A rather unusual observation was the migration of lipid-filled cells into the stratum germinativum of the overlying epithelium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.1976.tb01756.x
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  • 3
    Electronic Resource
    Electronic Resource
    Ultrastructural characteristics of mast cells from a canine mastocytoma maintained in vitro (1975)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral pathology & medicine 4 (1975), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract. Mast cells obtained from a canine mastocytoma were maintained in cell culture for a period of 11 weeks. Samples of these cells were harvested for electron microscopic examination after 9 weeks in vitro. Although the overall morphologic appearance was sufficient to allow their identification as mast cells, the tumor cells differed in several respects from descriptions of normal tissue mast cells.In contrast to normal tissue mast cells, the tumor cells exhibited peripheral accumulations of microfilaments, randomly dispersed microtubules, and small clusters of smooth endoplasmic reticulum. The tumor mast cells also presented three different granule types: spherical granules with an amorphous and electron dense matrix; irregularly shaped granules possessing a limiting external membrane and an internal matrix containing laminated and/or coiled structures; and granules containing loosely coiled, unorganized membrane structures similar in appearance to myelin whorls.The canine mastocytoma is an excellent source of mast cells as they can be obtained in large numbers without contamination by extraneous cell types and the cells can be maintained in vitro for extended periods of time.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.1975.tb01864.x
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  • 4
    Electronic Resource
    Electronic Resource
    Ultrastructural cytochemistry of complex carbohydrates in osteoblasts, osteoid, and bone matrix (1983)
    Springer
    Calcified tissue international 35 (1983), S. 309-319 
    Details
    ISSN: 1432-0827
    Keywords: Calcification ; Glycosaminoglycan ; Glycoprotein ; Collagen ; Acid phosphatase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Glycosaminoglycans (GAGs) and glycoproteins are essential components for osteogenesis. We have examined rat osteoblasts, osteoid, transitional zone, and fully calcified bone matrix, utilizing Spicer's high-iron diaminethiocarbohydrazide-silver protein (HID-TCH-SP) method for sulfated glycoconjugates and Thiéry's periodate-TCH-SP (PA-TCH-SP) method for vicinal glycol-containing glycoconjugates. HID-TCH-SP stained cytoplasmic granules of osteoblasts. Stain deposits in the extracellular matrix were observed in decreasing amounts in osteoid, the transitional zone, and fully calcified bone matrix. Enzyme digestion with testicular hyaluronidase removed most HID-TCH-SP stain deposits. PA-TCH-SP staining was observed with increasing intensity in rough endoplasmic reticulum, Golgi saccules, and cytoplasmic granules. Collagen fibrils in osteoid were weakly stained with PA-TCH-SP, and their staining appeared even weaker in fully calcified bone matrix. In contrast, collagen fibrils in calcified cartilage stained intensely with the PA-TCH-SP method. Focal circular profiles (0.1–0.5µm in diameter), which lacked collagen fibrils but reacted moderately with PA-TCH-SP, were frequently seen in the transitional zone and fully calcified bone matrix, but were only occasionally present in osteoid. The presence of testicular hyaluronidase-resistant GAG and acid phosphatase in these focal areas suggests that they represent sites of GAG degradation. The eventual loss of HID-TCH-SP staining in the bone matrix suggests that removal of sulfated glycoconjugates may be a requisite for expansion of initial calcification sites and/or complete calcification.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02405052
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  • 5
    Electronic Resource
    Electronic Resource
    Ferrocyanide enhancement of concanavalin A-ferritin and cationized ferritin staining blood cell surface glycoconjugates (1979)
    Springer
    Journal of molecular histology 11 (1979), S. 379-389 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Synopsis Ferrocyanide was used to enhance cationized ferritin and concanavalin A-ferritin (Con A-ferritin) staining of surface glycoconjugates of peripheral blood and bone marrow cells from rabbits and humans. The glutaraldehyde-fixed cells were stained with Con A-ferritin or cationized ferritin and then exposed to a ferrocyanide solution. The resulting cuboidal and irregular stain deposits averaged 50 nm in diameter when viewed with the transmission (TEM) and scanning electron microscope (SEM). Rabbit blood cells demonstrated more Con A binding sites than human blood cells and the decrease in binding sites observed with maturation of human granulocytic and erythrocytic cells was not evident in rabbit cells. Differences in binding of cationized ferritin to rabbit and human cell surfaces were less prominent than that observed for Con A. These results extend previous studies of blood cell surface glycoconjugates and demonstrate that ferrocyanide enhancement significantly facilitates SEM evaluation of Con A-ferritin and cationized ferritin bound to cell surfaces.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01002766
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  • 6
    Electronic Resource
    Electronic Resource
    Ultrastructural cytochemistry of proteoglycans associated with calcification of shark cartilage (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 208 (1984), S. 149-158 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Proteoglycans (PGs) as well as sulfated glycosaminoglycans (GAGs) are closely associated with cartilage calcification. An inner zone of endoskeletal tesserae of sharks is composed of a unique calcified hyaline cartilage. Initial calcification can be seen in the cartilage close to the inner zone. We have ultrastructurally examined shark, Triakis scyllia, noncalcifying, calcifying, and calcified cartilage using the tannic acid-ferric chloride (TA-Fe), the high iron diamine (HID), and the HID-thiocarbohydrazide-silver proteinate (HID-TCH-SP) methods for localization of sulfated complex carbohydrates. In noncalcifying cartilage, TA-Fe and HID strongly stained matrix granules which were round, ovoid, elongated, or irregularly shaped and presumably represented PG monomers. The size and staining intensity of the reactive matrix granules progressively decreased in calcifying cartilage toward the calcification front of the calcified cartilage. Similarly, a progressive decrease in the size of the HID-TCH-SP stain deposits in the matrix granules was observed in the calcifying cartilage close to the calcification front and was interpreted as a decrease in length of sulfate containing GAG chains. In the calcified cartilage, the highly calcified areas were often localized in the calcification front and contained few or no small HID-TCH-SP stain deposits, whereas the weakly calcified regions contained more stain deposits. These results indicate that partial and complete degradation of sulfated GAGs and/or PGs may be a requisite for calcification of shark cartilage.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092080202
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  • 7
    Electronic Resource
    Electronic Resource
    Ultrastructural distribution of sulfated complex carbohydrates in elastic cartilage of the young rabbit (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 207 (1983), S. 547-556 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Sulfated glycosaminoglycans are an integral component of elastic cartilage. We have investigated the ultrastructural distribution of sulfated complex carbohydrates (CC)in the mature cartilage and the perichondrium of young rabbit auricles using the high iron diamine-thiocarbohydrazide-silver proteinate (HID-TCH-SP) and the tannic acid-ferric chloride (TA-Fe) methods. In the mature cartilage, HID-TCH-SP stained intracellular Golgi saccules of the mature face, secretory granules, and the extracellular matrix granules, but staining was not discernible in collagen fibrils and osmiophilic elastic fibers consisting of only amorphous elastin. The HID and TA-Fe staining were similarly observed in matrix granules, whereas the elastic fibers and collagen fibrils lacked the staining. The pericellular matrix granules had a diameter of 34 ± 5 nm (mean ± SD; n = 30). Thiéry's periodate-TCH-SP (PA-TCH-SP) method stained vicinal glycol-containing CC in collagen fibrils but failed to stain matrix granules and elastic fibers. In the perichondrium, HID-TCH-SP staining of the organelles was less intense in the flattened chondrocytes when compared with those in large mature chondrocytes. The extracellular HID and HID-TCH-SP staining were observed in the matrix granules. The diameter of pericellular matrix granules (19 ± 4 nm, mean ± SD; n = 30) was significantly smaller when compared to those in the mature cartilage (P 〈 0.001). The HID-TCH-SP staining was closely associated with collagen fibrils. However, the staining was not seen in collagen fibrils and osmiophilic elastic fibers consisting of elastin and microfibrils. The PA-TCH-SP method stained collagen fibrils and microfibrils but did not stain the amorphous elastin. Thus these studies demonstrate that sulfated CC are packaged in chondrocyte secretory granules and are released into the extracellular matrix to form matrix granules, but are not incorporated into collagen fibrils and elastic fibers.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092070403
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  • 8
    Electronic Resource
    Electronic Resource
    Fine structural features of developing leg discs of the blowfly, Sarcophaga bullata (Parker) (1968)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 126 (1968), S. 349-363 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The mesothoracic leg discs of the blowfly, Sarcophaga bullata (Parker) were studied by electron microscopy during the third larval instar. As the peripodial membrane and separation form, the cells of the disc become elongated and perpendicularly oriented toward the separation. The cellular organelles do not undergo significant changes until the late third instar. At this time, there is a significant increase in the amount of granular endoplasmic reticulum and in the number of inclusions, particularly in those cells located more deeply in the disc. Nucleolar enlargement and an increase in the number of mitochondria are also observed at this time.The cells at the border of the disc form a columnar epithelium whose surface develops microvilli-like projections. These projections. These projections reach their maximum development towards the end of third instar.It is suggested that some of the observed changes may represent a phenotypic expression of secretion of cuticular material.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051260305
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  • 9
    Electronic Resource
    Electronic Resource
    The fine structure of the human corpus luteum of early pregnancy and during the progestational phase of the menstrual cycleThis work was supported by general research funds to the Department of Anatomy, Georgetown University and, in part, by a contract (NIH-69-2218) with the National Institute of Child Health and Human Development, Department of Health, Education and Welfare. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 127 (1970), S. 37-69 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The fine structure of granulosa lutein cells and theca lutein cells of human corpora lutea of early pregnancy and during the progestational phase of the menstrual cycle (i.e., 1 day, 7 days and 9 days after estimated ovulation) is described. Granulosa lutein cells of early pregnancy are distinguished from theca lutein cells in having: (1) a more homogeneous, electron-lucent nuclear matrix, (2) enlarged pleomorphic mitochondria with irregularly-shaped, osmiophilic inclusions, (3) numerous isolated regions of the Golgi complex, (4) abundant whorls of granular and agranular endoplasmic reticulum, (5) a folded-membrane complex, (6) numerous bundles of 50 Å filaments and (7) patches of elongated microvilli bordering intercellular and intracellular canaliculi.Furthermore, granulosa lutein cells of early pregnancy are distinguished from granulosa lutein cells of corpora taken during the progestational stage of the menstrual cycle by the greater abundance of granular and agranular endoplasmic reticulum, the presence of concentric membranous whorls and large spherical mitochondria, increases in membrane-bound granules and a more extensive development of intracellular canaliculi. These differences are related to the high titers of serum gonadotrophin (s) during early gestation.The morphology of human corpora lutea is compared with steroidogenic tissues of other species and is correlated with the capacity of human corpora lutea to synthesize estrogens in addition to progestogens. Based upon morphological evidence, it is suggested that in addition to elaborating steriod hormones, corpora lutea may also secrete a proteinaceous product, perhaps relaxin.This work was presented as a demonstration to the eighty-second annual session of the American Association of Anatomists in April, 1969. An abstract has been published (Anat. Rec., 163: 336, '69).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001270105
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  • 10
    Electronic Resource
    Electronic Resource
    Ultrastructural visualization of complex carbohydrates in eosinophilic leukocytes (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 165 (1982), S. 53-67 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Complex carbohydrates in eosinophils from human, rabbit, and rat marrow were identified and localized by cytochemical and radioautographic methods. The high iron diamine-thiocarbohydrazide-silver proteinate (HID-TCH-SP), low iron diamine (LID)-TCH-SP, and periodate (PA)-TCH-SP methods were used for the localization of sulfate, sulfate and carboxyl, and vicinal glycol-containing complex carbohydrates, respectively. Golgi vesicles and small precursor granules (0.2-0.4 μm in diameter) demonstrated strong HID-TCH-SP staining and labeled intensely after a 10-minute pulse with 35 SO42-. Crystalloid-free or immature specific granules (0.5-0.9 μm in diameter) labeled heavily after a 60-minute incubation and 60-minute chase with 35SO42-. Immature granules were graded according to their HID-TCH-SP staining: Type 1 granules demonstrated strong rim staining and similar or somewhat less central staining, whereas type 2 granules only demonstrated rim staining, and type 3 granules lacked staining. Fully mature crystalloid-containing granules lacked staining. LID-TCH-SP similarly stained the HID-positive sulfated material in cytoplasmic granules. PA-TCH-SP stained some Golgi vesicles and diffusely stained all precursor granules and type 1 granules. Weaker staining was observed in type 2 granules and staining was very weak or absent in type 3 and crystalloid-containing granules. In early eosinophils, tubulovesicular structures (TVS) were observed rosetting and contacting precursor and type 1 granules. These TVS contained material with strong PA-TCH-SP staining but lacked HID-TCH-SP or LID-TCH-SP-reactive acidic glycoconjugates. Flattened Golgi saccules of early eosinophils stained weakly or not at all with the PA-TCH-SP method. Small granules and TVS in late (bilobed) eosinophils displayed PA-TCH-SP reactivity and lacked HID-TCH-SP staining but differed from TVS in early eosinophils in that they were not associated as rosettes with specific granules. These results indicate that sulfated and vicinal glycol-containing complex carbohydrates are differently distributed in immature specific granules of eosinophils and presumably become masked to staining as the granule matures.
    Additional Material: 15 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001650106
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