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  • 1
    Electronic Resource
    Electronic Resource
    Microlithiasis in parotid sialadenosis and chronic submandibular sialadenitis is related to the microenvironment: an ultrastructural and microanalytical investigation (1998)
    Oxford, U.K. and Cambridge, USA : Blackwell Science Ltd
    Histopathology 32 (1998), S. 0 
    Details
    ISSN: 1365-2559
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Microlithiasis was investigated in parotid sialadenosis and chronic submandibular sialadenitis to determine if it relates to the glandular microenvironment as has been found experimentally.〈section xml:id="abs1-2"〉〈title type="main"〉Methods and resultsSemithin sections were stained by a mixture of methylene blue and Azure II followed by basic fuchsin, which stains calcified parts of microliths red and organic parts green, and ultrathin sections were examined electron microscopically and microanalytically. Microliths in sialadenosis were found in periacinar stroma, in which necrotic acinar cells were found, and in parenchyma, and consisted of consolidated organic material with little or no crystalline calcium. Microliths in sialadenitis were found in stroma, particularly around intercalary ducts, in lumina and in parenchyma, and contained much crystalline calcium. Macrophages enclosed some microliths.〈section xml:id="abs1-3"〉〈title type="main"〉ConclusionsThe paucity of calcium in microliths in sialadenosis and the abundance in sialadenitis relates to the glandular calcium. The periacinar distribution of microliths in sialadenosis possibly relates to formation in periacinar necrotic debris. The distribution of microliths in sialadenitis around intercalary ducts possibly relates to formation in matrix vesicles formed from atrophic parenchyma, and in lumina to formation in stagnant secretory material. Microliths appear to be scavenged by macrophages. Thus the experimental finding that salivary microlithiasis relates to the microenvironment pertaining in humans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2559.1998.00432.x
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  • 2
    Electronic Resource
    Electronic Resource
    Characteristics of the cancellous bone of edentulous mandibles (1997)
    Copenhagen : Munksgaard International Publishers
    Clinical oral implants research 8 (1997), S. 0 
    Details
    ISSN: 1600-0501
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Trabecular bone volume and trabecular connectivity (trabecular bone pattern factor) of edentulous mandibles were examined using undecalcified bone sections from the region of the 1st premolar to investigate atrophyrelated changes in mandibular cancellous bone. The mean trabecular bone volume was 21.8% in female mandibles and 36.4% in male mandibles. The mean trabecular bone pattern factor was -0.22 mm−1 for female mandibles and -2.29 mm−1 for male mandibles. The difference between the sexes was statistically conspicuous for both parameters, but did not attain statistical significance. A notable fact was the extreme range of variation in both trabecular bone volume and trabecular connectedness. A difference of 65% between the highest and the lowest trabecular bone volumes measured in the present study (min, 7.6%; max, 73.6%, both male) reflects the possible variation in trabecular density of edentulous mandibles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1600-0501.1997.080207.x
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  • 3
    Electronic Resource
    Electronic Resource
    Titanium implant insertion into dog alveolar ridges augmented by allogenic material (1994)
    Copenhagen : Munksgaard International Publishers
    Clinical oral implants research 5 (1994), S. 0 
    Details
    ISSN: 1600-0501
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The purpose of this investigation was to evaluate whether titanium endosseous implants would osseointegrate in dog alveolar ridges augmented by allogenic material. In 8 dogs en bloc resection, including 2 premolars, was performed bilaterally in the maxilla and the mandible. After a healing period of 6 weeks allogenic, demineralized and lyophilized dentin or bone was implanted subperiosteally. Titanium implants were installed 5.5 months later in some of the regions. Light and fluorescence microscopic evaluation revealed fibrous encapsulation of the implanted allogenic material, no osteoinduction and only minimal osteoconduction. few multi-nuclear giant cells and a sparse inflammatory reaction. The titanium implants healed mainly by fibrous encapsulation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1600-0501.1994.050404.x
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  • 4
    Electronic Resource
    Electronic Resource
    The role of early versus late removal of GTAM® membranes on bone formation at oral implants placed into immediate extraction sockets. An experimental study in dogs. (1993)
    Copenhagen : Munksgaard International Publishers
    Clinical oral implants research 4 (1993), S. 0 
    Details
    ISSN: 1600-0501
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The purpose of this study was to compare the effect of early versus late removal of expanded polytetrafluoroethylene (e-PTFE) membranes on bone formation at oral implants. Thirty Brånemark™ fixtures were placed into immediate extraction sockets with buccal bone dehiscences augmented by e-PTFE membranes. At 4 weeks, the membranes and underlying soft tissues were removed from 5 implants, but at 16 sites only the membranes were extirpated. In 9 sites, the membranes remained in place during the healing period. Sixteen weeks after fixture insertion, the sites in which the membrane was retained (MRET) showed an average of 5.2 mm of clinical bone height increase (lOO% of bone fill). For sites where the membrane together with underlying soft tissues were removed (MRB), the corresponding value was 2.0 mm (42% of bone fill. Implants at which only the membrane was removed (MR) showed the least clinical bone height increase (1.0 mm). resulting in 21% coverage of original threads. Histometric measurements verified that the MRET sites had the least distance from the top of the fixture to the newly formed bone level (0.4 mm). However, in contrast with the clinical findings, the histometric analyses showed that the MRB group had the greatest remaining bone defect (3.3 mm). The clinical and histometric results of the MRET group were statistically better, though, compared with those of the other two groups. Biopsies, removed from beneath the membranes, revealed slightly inflamed connective tissue, containing spicules of newly formed bone, indicating that more bone might have been created if the membranes had been retained longer. The current study thus showed that maximum bone formation around oral implants was created if the membrane augmentation material remained in place during the 16-week healing period. Early removal of membranes, with or without elimination of the underlying tissues resulted, however. in less newly formed bone and an incomplete defect bone fill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1600-0501.1993.040302.x
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  • 5
    Electronic Resource
    Electronic Resource
    Comparison of healed tissues adjacent to submerged and non-submerged unloaded titanium dental implants. A histometric study in beagle dogs. (1996)
    Copenhagen : Munksgaard International Publishers
    Clinical oral implants research 7 (1996), S. 0 
    Details
    ISSN: 1600-0501
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: This study involved histometry of the healed tissues around submerged and nonsubmerged dental implants in beagle dogs. In a split-mouth design, 19 submerged and 19 nonsubmerged commercially pure titanium implants, titanium plasma-sprayed in the bone anchoring part and smooth in the transmucosal portion were placed in the mandibles of 6 dogs. Oral hygiene was performed 3 times weekly. After 3 months of healing, transmucosal abutments were inserted in the submerged implants. Six weeks after second stage surgery, the dogs were sacrificed and specimens obtained and processed for histology and histometry. Using a light microscope and a digitizing pad, the distance from implant top to mucosa border (DIM), the extent of epithelial downgrowth (ED), the attachment level (AL). the length of connective tissue contact (CTC) and the distance of the first coronal alveolar bone contact from the implant top (DIB) were measured at the mesial and distal aspects. Means+standard deviations for submerged and nonsubmerged implants were calculated, with the dog being the unit of measure. No statistically significant differences between submerged and nonsubmerged implants here found for DIM, CTC and DIB. However, significant differences were observed for ED and AL. This study in beagle dogs indicates that the apical extension of the peri-implant epithelium is significantly greater and the attachment level significantly lower adjacent to submerged implants with second-stage transmucosal abutments than in nonsubmerged, one-stage implants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1600-0501.1996.070102.x
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  • 6
    Electronic Resource
    Electronic Resource
    A pilot study comparing screw-shaped implants. Surface analysis and histologic evaluation of bone healing. (1996)
    Copenhagen : Munksgaard International Publishers
    Clinical oral implants research 7 (1996), S. 0 
    Details
    ISSN: 1600-0501
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The purpose of this study was to compare surface treatment and bone formation adjacent to 2 screw shaped implants of similar design manufactured by two different companies. The test implants were manufactured by SERF (Decines, France), while the controls were manufactured by Nobelpharma (Göteborg, Sweden). The surface of 3 standard 3.75mm test and 3 standard 3.75mm control implants were investigated by means of scanning electron microscopy SEM, X-ray micro-analysis, electron spectroscopy for chemical analysis (ESCA) and surface topography analysis. There was a microscopic difference on the thread design (SEM). Test threads were flat at the edge, while controls appeared rounded at the edge of the threads. Tests and controls were made of commercially pure titanium, with a regular topography. Results of ESCA indicated that the carbon peak for SERF implants was slightly higher than for the Brånemark implants. 5 test and 5 control implants were installed into the epiphyseal head of the femur of 2 ewes using a standardized surgical technique. In order to stain the bone for histologic analysis, oxytetracycline injections were given 17 and 8 days before the animals were sacrificed. The animals were sacrificed 12 weeks after implant placement. Histomorphometric analysis indicated that there was an average bone to implant contact of 68% for the test implants and 61% for the controls. There were no statistical differences between tests and controls. The preliminary results of this pilot study indicated that early bone healing for the 2 screw shaped implants investigated were similar.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1600-0501.1996.070414.x
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  • 7
    Electronic Resource
    Electronic Resource
    DNA flow cytometry of reclassified subtypes of malignant salivary gland tumors (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral pathology & medicine 23 (1994), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Malignant salivary gland tumors are rare, constitute a heterogeneous group and are often difficult to diagnose histologically. This is borne out by the fact that in the present study 43.2% of 118 salivary gland tumors originally diagnosed as mucoepidermoid, acinic cell and adenoid cystic carcinomas had their original diagnosis altered upon reclassification. Patients with confirmed adenoid cystic carcinomas had a much worse prognosis than those with mucoepidermoid and acinic cell carcinomas.DNA flow cytometry showed that very few of the above mentioned three types of malignant neoplasms revealed aneuploid DNA stemlines, indicating that this is not a relevant prognostic tumor marker within the groups. However, several of the tumors that had their diagnosis changed, mostly to undifferentiated adenoor squamous cell carcinomas, showed aneuploid DNA stemlines. The survival time of patients with aneuploid tumors was considerably reduced compared to those with diploid tumors. Among confirmed acinic cell, mucoepidermoid and adenoid cystic carcinomas the S-phase fraction was a significant prognostic factor, as it was among all tumors examined. This indicates that DNA aneuploidy and S-phase fractions are potential prognostic factors for malignant salivary gland tumors, and that DNA flow cytometry may assist the characterization of such tumors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.1994.tb00064.x
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  • 8
    Electronic Resource
    Electronic Resource
    The spectrum of giant cells in tumours of the salivary glands: an analysis of 11 cases (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral pathology & medicine 26 (1997), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In view of the different terminology for salivary gland tumours with giant cells, eleven cases were analysed by histopathology and immunocytochemistry. Four cases (three pleomorphic adenomas, one carcinosarcoma in a pleomorphic adenoma) were classified as having a foreign-body giant cell reaction, and five cases (two mucoepidermoid carcinomas, one acinic cell carcinoma, two carcinomas in pleomorphic adenomas) as having a sarcomatoid osteoclast-like giant cell reaction. In two further cases a giant cell tumour and a giant cell granuloma were associated with carcinomas in pleomorphic adenomas. All giant cells showed characteristic expression of CD68 as a typical marker for histiocytes and macrophages with their origin in mononuclear haematopoetic stem cells. There was no evidence for an epithelial origin of the giant cells because all those examined had a negative reaction to cytokeratin. Foreign-body cells were characterized by cytoplasmic vacuoles and irregularly dispersed nuclei. They showed a focally circumscribed reaction mostly outside the connective tissue pseudocapsule of the tumours. The sarcomatoid osteoclast-like giant cell reactions in carcinomas were distinctly intermingled with the carcinomatous patterns. In contrast, the associated osteoclast-like giant cell tumour was distinctly separate from the salivary gland tumour tissue and was composed of numerous larger osteoclast-like giant cells with a greater number of nuclei (more than 20): these giant cells were uniformly distributed throughout the tumour tissue. The giant cell granuloma was also separate from the carcinoma and was composed of nests of smaller, more irregularly distributed giant cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.1997.tb00244.x
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  • 9
    Electronic Resource
    Electronic Resource
    A method for the study of undecalcified bones and teeth with attached soft tissues (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral pathology & medicine 11 (1982), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A new sawing-grinding method is described for the histological evaluation of jaw bones with teeth or bones containing implants (ceramic or metallic). The undecalcified bone is embedded in acrylic resin and sawed at 100 to 150 μm. The slices are ground automatically by a special machine to a thickness of 5–10 μm. The usually employed staining procedures for hard plastic embedded tissues may be used. Plaque, fillings, crowns, bridges, implants and soft tissues are preserved in situ. Macroscopic and microscopic detail of good quality is preserved for histological and morphometrical evaluation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.1982.tb00172.x
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  • 10
    Electronic Resource
    Electronic Resource
    Hydroxyapatite-ceramic for juxta-articular implantation (1992)
    Springer
    Journal of materials science 3 (1992), S. 345-351 
    Details
    ISSN: 1573-4838
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract The histocompatibility of hydroxyapatite-ceramic (HAC) has been proven extensively. For the reconstruction of juxta-articular cancellous bone defects with this synthetic material, the mechanical properties of the HAC-bone regeneration complex needed to be investigated. In order not to alter the specific ability of the articular structures to distribute and absorb loading stress, the physiological force-transmitting performance of the subchondral zone must be achieved by filling the defect within HAC. This study deals with the influence of a physiological load on the remodelling within HAC-filled subchondral bone defects. As orientation is the important factor affecting the physical properties of hard tissue, we show the morphological aspect of functional adaptation of the hydroxyapatite-bone compound determined by the orientation of the bone collagen fibres. By biomechanical methods, the elastic properties of the resulting ceramo-osseous regeneration complex were tested. Reproducible subchondral bone defects were prepared in medial femoral condyles of rabbits, leaving a 0.5 mm coplanar layer of bone and cartilage. The defects were filled with granules of HAC. Polarizing microscopy revealed the dynamical aspect of the bony integration of the material and the remodelling process under physiological locomotion. It showed a rapid ongrowth of collagen fibres on the ceramic surface. By its increasing orientation to domains from woven texture to economical trabecular architecture, the load-bearing facility is documented. Indenting the articular surface on an impressive force testing machine 18 months after HAC implantation proved the equal elastic response of the ceramo-osseous regeneration complex with the overlying structures in comparison with the integrity of not-operated femoral condyles. When integrated by bone, HAC fulfils in our dynamic animal model physiological demands even in large bone defects close to articular surfaces.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00705366
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