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  • 1
    Electronic Resource
    Electronic Resource
    Atomic force microscope measurements of nucleosome cores assembled along defined DNA sequences (1993)
    s.l. : American Chemical Society
    Biochemistry 32 (1993), S. 8390-8396 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00084a002
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  • 2
    Electronic Resource
    Electronic Resource
    Multistage affinity cross-flow filtration: mathematical modelling and analysis (1996)
    Springer
    Bioprocess and biosystems engineering 15 (1996), S. 99-104 
    Details
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A multistage affinity cross-flow filtration (mACFF) process for protein purification is proposed. The process is mathematically modelled taking into account a case of rapid equilibrium binding of a target protein to its macroligand. The process performance, i.e., dimensionless breakthrough volume (Q b + )and recovery yield (REC) to obtain a desired purity is analysed by computer simulations. The results indicate that Q b + increases with the increase of stage number (n) due to the increase of affinity binding efficiency. In addition, REC also increases with the increase of n, especially for lower affinity systems, even though the feed loading is the same as the corresponding breakthrough volume that increases with n. Thus both feed loading and recovery yield can be enhanced by raising the stage number. Incompletely permeable membranes reject the target and contaminant proteins. So they delay the appearance of the breakthrough point and compromise the contaminant washing efficiency. Hence although Q b + increases with the increase of membrane rejection coefficient (R), REC decreases when the feed loading equals that of Q b + . However, when the feed loading is kept unchanged and equals Q b + at R=0, REC does not decrease, but slightly increases with the increase of R. This result indicates that incompletely permeable membranes may also be employed for the mACFF process. In general, the model gives a predictive evaluation of the mACFF process successfully.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00372984
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  • 3
    Electronic Resource
    Electronic Resource
    Modelling and analysis of the continuous affinity-recycle extraction process: a case of specific elution with low molecular weight competitive inhibitor (1995)
    Springer
    Bioprocess and biosystems engineering 13 (1995), S. 205-210 
    Details
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The continuous affinity-recycle extraction (CARE) process of specifie elution with low molecular weight competitive inhibitor is mathematically modelled taking into account the presence of membrane rejections to the components in a crude broth. The process performance, defined as purification factor (PF) and recovery yield (REC), is analyzed by computer simulations. The results show that for constant affinity systems (ligate and ligand as well as inhibitor) and operating conditions an optimal value of the inhibitor concentration exists to give maxima of REC and PF, and the optimal value decreases with the increase of the affinity binding constant of ligate and inhibitor. Although the increase in affinity-recycle flow rate results in the decrease of PF, an optimal value of the affinity-recycle flow rate exists to show a maximum of REC. Hence in the process design the selection of the affinity-recycle flow rate is also of importance to obtain higher REC and PF simultaneously. The consideration of membrane rejections will in practice be useful to analyse the separation of a binary broth using ultrafiltration membranes which reject to the components. For a multicomponent broth, however, membranes without rejection to all components should be employed to simplify the process design and optimization. In general, the model is useful to design a CARE process using nonporous microparticles or macromolecules as affinity supports.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00367255
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  • 4
    Electronic Resource
    Electronic Resource
    Gain dynamics of two-wavelength Ti:sapphire femtosecond laser (1999)
    Springer
    Applied physics 69 (1999), S. 211-216 
    Details
    ISSN: 1432-0649
    Keywords: PACS: 42.50; 42.55.R; 42.60.F; 42.65
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract. We report the theory and experiments of a two-wavelength bi-tuning femtosecond Ti:sapphire laser. The cross-mode-locked gain competition is described with numerical solution and operation of the laser is accomplished in which two trains of femtosecond pulses cross in the Ti:sapphire rod. For this reason, a cross-bearings technique is used. The laser resonator is a six-mirror bi-cavity, and two beams of pump lights cross in Ti:sapphire crystal. Two trains of sub-30 fs cross-mode-locked pulses have been generated with independent tunable wavelength when the pump power of the argon laser is approximately 5 W. As the design of the bi-cavity is fine, not only strong gain competition is overcome, but also stable output characteristics are performed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003400050796
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  • 5
    Electronic Resource
    Electronic Resource
    Extending Shelf Life of Fresh-cut Pears (2000)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 65 (2000), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effects of various treatments were evaluated for extending shelf-life of fresh-sliced pears. Sliced Anjou pears had browning-free color for 30 d by dipping with 1.0% ascorbic acid and 1.0% calcium lactate, but texture was soft with juice leakage. The combination treatment of 0.01% 4-hexylresorcinol (4-HR), 0.5% ascorbic acid and 1.0% calcium lactate can provide 15 to 30 d shelf-life for Anjou, Bartlett, and Bosc pears when the pears are sliced at an average ripeness of 43, 49, and 38 Newton respectively, with 2 min dipping, partial vacuum packaging, and 2 to 5 °C storage. 4-HR residual content ranged from 1 to 7 ppm after 14 d storage. Panelists could detect a flavor difference between 0.01% 4-HR treated pears and controls.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.2000.tb15976.x
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  • 6
    Electronic Resource
    Electronic Resource
    Synthesis of novel targeted pro-prodrugs of anthracyclines potentially activated by a monoclonal antibody galactosidase conjugate (part 1)
    Amsterdam : Elsevier
    Bioorganic & Medicinal Chemistry Letters 2 (1992), S. 1093-1096 
    Details
    ISSN: 0960-894X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology , Medicine
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/S0960-894X(00)80625-3
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  • 7
    Electronic Resource
    Electronic Resource
    Synthesis of a phenyl β-avobioside derivative of the dissacharide component of avoparcins
    Amsterdam : Elsevier
    Carbohydrate Research 232 (1992), S. 107-115 
    Details
    ISSN: 0008-6215
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/S0008-6215(00)90997-2
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  • 8
    Electronic Resource
    Electronic Resource
    Synthesis of disaccharide fragments of dermatan sulfate
    Amsterdam : Elsevier
    Carbohydrate Research 195 (1989), S. 39-50 
    Details
    ISSN: 0008-6215
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0008-6215(89)85086-4
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  • 9
    Electronic Resource
    Electronic Resource
    SYSTEMIC ACQUIRED RESISTANCE (2004)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Phytopathology 42 (2004), S. 185-209 
    Details
    ISSN: 0066-4286
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Biology
    Notes: Systemic acquired resistance (SAR) is a mechanism of induced defense that confers long-lasting protection against a broad spectrum of microorganisms. SAR requires the signal molecule salicylic acid (SA) and is associated with accumulation of pathogenesis-related proteins, which are thought to contribute to resistance. Much progress has been made recently in elucidating the mechanism of SAR. Using the model plant Arabidopsis, it was discovered that the isochorismate pathway is the major source of SA during SAR. In response to SA, the positive regulator protein NPR1 moves to the nucleus where it interacts with TGA transcription factors to induce defense gene expression, thus activating SAR. Exciting new data suggest that the mobile signal for SAR might be a lipid molecule. We discuss the molecular and genetic data that have contributed to our understanding of SAR and present a model describing the sequence of events leading from initial infection to the induction of defense genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.phyto.42.040803.140421
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  • 10
    Electronic Resource
    Electronic Resource
    Evaluation of Subsurface Oxygen Sensors for Remediation Monitoring (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Ground water monitoring & remediation 16 (1996), S. 0 
    Details
    ISSN: 1745-6592
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: Continuous remediation monitoring using sensors is potentially a more effective and inexpensive alternative to current methods of sample collection and analysis. Gaseous components of a system are the most mobile and easiest to monitor. Continuous monitoring of soil gases such as oxygen, carbon dioxide, and contaminant vapors can provide important quantitative information regarding the progress of bioremediation efforts and the area of influence of air sparging or soil venting. Laboratory and field tests of a commercially available oxygen sensor show that the subsurface oxygen sensor provides rapid and accurate data on vapor phase oxygen concentrations. The sensor is well suited for monitoring gas flow and oxygen consumption in the vadose zone during air sparging and bioventing. The sensor performs well in permeable, unsaturated soil environments and recovers completely after being submerged during temporary saturated conditions. Calibrations of the in situ oxygen sensors were found to be stable after one year of continuous subsurface operation. However, application of the sensor in saturated soil conditions is limited. The three major advantages of this sensor for in situ monitoring arc as follows: (1) it allows data acquisition at any specified time interval; (2) it provides potentially more accurate data by minimizing disturbance of subsurface conditions; and (3) it minimizes the cost of field and laboratory procedures involved in sample retrieval and analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1745-6592.1996.tb00577.x
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