ISSN:
0173-0835
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
This paper describes the visualization of the low density lipoprotein (LDL) receptor by ligand blotting technique using an anti-LDL-enzyme linked immunosorbent assay (ELISA). Solubilized membrance proteins from bovine adrenal cortex are subjected to sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis, transferred to nitrocellulose paper and incubated with LDL. The combination of peroxidase-con-jugated second antibody and 4-chloro-naphthol/H2O2 allows rapid development of colored bands where LDL was bound to the receptors. Saturation of the LDL binding to the LDL receptor can be demonstrated. This highly sensitive and rapid ELISA avoids the need for continuous supplies of iodinated antibodies.
Additional Material:
1 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150050610
Permalink
