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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Surface & Coatings Technology 60 (1993), S. 571-576 
    ISSN: 0257-8972
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Thin Solid Films 201 (1991), S. 123-135 
    ISSN: 0040-6090
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 2 (1988), S. 0 
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The scr genes located on plasmid pUR400 and responsible for sAucrose (Scr) metabolism of Escherichia coli K12 and other enteric bacteria have been cloned on a 9.3 kb DNA fragment. The different genes were mapped by transposon insertion mutagenesis, by restriction endonuclease and deletion mapping, and the corresponding gene products were identified. Besides the known structural genes scrA, coding for an EnzymellScr (45 kD) of the phosphoenolypyruvate-dependent phosphotransferase system (PTS), and scrB, coding for a sucrose 6-phosphate hydrolase (invertase) (55 kD), two new structural genes were discovered. Gene scrK apparently codes for an intracellular and ATP-dependent fructokinase (39 kD), while scry seems to code for a sucrose porin (58 kD) in the outer cell membrane. No genes for an Enzyme IIIScr of the PTS or for (a) glycosyltransferase(s) were detected. The four genes form an scr operon (gene order, scrK scrY scrA scrB, transcription from K to B), regulated by a repressor (gene scrR, 37 kD) and inducible by sucrose, fructose and fructose-containing oligosaccharides
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The nucleotide sequence of the structural gene, scrA, which codes for sucrose-specific EnzymellScr (EIIScr) of the phosphoenolpyruvate-dependent carbohydrate: phosphotransferase system (PTS), was determined. EllScr requires an Enzymelll, the product of the gene crr, for full activity. The gene scrA is preceded immediately by a classical Shine-Dalgarno sequence (AAGAGGGTA). It contains 1368 nucleotides with an increased GC-content (58%) corresponding to a polypeptide of 455 amino acid residues (Mr 47 500). The protein has the hydropathic profile (average hydropathy +0.82) of an integral membrane protein lacking extended α-helical structures and a signal peptide. Comparison with the sequence of the β-glucoside-specific Enzymell (EllBgl, 625 amino acids, Mr 66480; Bramley and Kornberg, 1987a; Schnetz et al., 1987) revealed strong homologies between EllScr and the first 458 residues of EllBgl. The 162 carboxyterminal residues of EllBgl, however, showed a high homology with the sequence of Enzymelll (Nelson et al., 1984), a homology also described recently by Bramley and Kornberg (1987b). The evolutionary and functional significance of the similarities with four other Enzymesll is discussed.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 5 (1991), S. 0 
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: During the molecular analysis of a plasmid-coded sucrose metabolic pathway of enteric bacteria, a gene, scrY, was found whose product, ScrY, had all the properties of a bacterial porin (Schmid et al, 1988). Loss of this protein (Mr 58kDa), localized in the outer membrane, led, as shown here, to an increase in the apparent Km for sucrose transport in whole cells from 10 μM in wild-type cells to 300 μM in mutant cells. This contrasts with the Km for sucrose phosphorylation as measured in membrane vesicles from mutant and wild-type cells, which remained unchanged at about 10 μM, and reflects the activity of the sucrose-specific Enzymell of the phosphoenolpyruvate-dependent carbohydrate:phosphotransferase system (PTS) responsible for uptake through the inner membrane. Furthermore, the presence of ScrY restored growth on maltodextrins in cells devoid of LamB, thus complementing the lack of this maltoporin. The amino acid sequence deduced from the DNA sequence was determined for the plasmid-coded and the ScrY porin coded in the chromosome of Klebsiella pneumoniae. Both show high identity (86%) to each other, and to the channel domain of LamB, further corroborating the conclusion that they constitute porins.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Sucrose-positive derivatives of Escherichia coli K-12, containing the plasmid pUR400, and of Klebsiella pneumoniae hydrolyse intracellular sucrose 6-phosphate by means of an invertase into d-glucose 6-phosphate and free d-fructose. The latter is phosphorylated by an ATP-dependent fructokinase (gene scrK of an scr regulon) to d-fructose 6-phosphate. The lack of ScrK does not cause any visible phenotype in wild-type strains of both organisms. Using genes and enzymes normally involved in d-arabinitol metabolism from E. coli C and K. pneumoniae, derivatives of E. coli K-12 were constructed which allowed the identification of scrK mutations on conventional indicator plates. Cloning and sequencing of scrK from sucrose plasmid pUR400 and from the chromosome of K. pneumoniae revealed an open reading frame of 924 bp in both cases — the equivalent of a peptide containing 307 amino acid residues (Mr, 39 and 34 kDa, respectively, on sodium dodecyl sulphate gels). The sequences showed overall identity among each other (69% identical residues) and to a kinase from Vibrio alginoiyticus (57%) also involved in sucrose metabolism, lower overall identity (39%) to a d-ribose-kinase from E. coli, and local similarity to prokaryotic, and eukaryotic phosphofructokinases at the putative ATP-binding sites.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Fatigue & fracture of engineering materials & structures 13 (1990), S. 0 
    ISSN: 1460-2695
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract— Solid solution hardening and strain hardening are the dominating strengthening mechanisms to achieve a high yield stress and ultimate tensile strength in Cr-Mn-N steels for drill collars. The fatigue limit can be improved more effectively by solid solution hardening than by strain hardening, but the attainable hardness due to soluble elements is restricted by other metallurgical demands. Strain hardening significantly increases the strength and the fatigue limit is improved too, but to a lesser extent. The reason for this behaviour is the introduction of internal stresses of the I, II and III kind by forging the drill collars. This results in macroscopic stresses which are varying over the cross section and in stresses of a microscopical scale which lead to an early loss of linearity in the elastic line of the stress strain curve and to the well known Bauschinger effect. Cyclic softening and hardening is accompanied by a rearrangement of the dislocation structure as revealed by transmission electron microscopy. The results indicate that a multiaxial and homogeneous cold working to produce a stable dislocation structure and to avoid large directional internal stresses is extremely important to achieve high fatigue strengths. Crack growth and crack closure measurements were performed for determination of the effective cyclic threshold stress intensity range for evaluation of the influence of the grain size on the fatigue limit.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 345-346 (Aug. 2007), p. 685-688 
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Pressure casting dies are subjected to a large number of thermal as well as mechanical loadcycles, which are leading to a characteristic thermally induced crack network on the die surface. As atypical representative for a die material the cyclic thermo-mechanical behavior of the hot work toolsteel grade 1.2343 (X38CrMoV5-1) is investigated both experimentally as well as numerically. Onthe one hand the information from isothermal compression-tension tests is used in a subsequentanalysis to calibrate a constitutive model that takes into account the characteristic combinedisotropic-kinematic hardening/softening of the material. On the other hand the non-isothermalmechanical response of the material to thermal cycles is characterized by means of a periodic laserpulse applied to a small plate-like specimen which is cooled on the back. The residual stressesdeveloping at the surface of the irradiated region of the specimen are determined ex-situ by means ofX-ray diffraction. The obtained values agree well with the results of an accompanying finite-elementstudy. This information is used to verify the calibrated constitutive model. The material law is finallyused for the prediction of stresses and strains in a die
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 166-169 (July 1994), p. 181-186 
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-1459
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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