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  • 1
    Electronic Resource
    Electronic Resource
    Simultaneous processing of substrate-dependent monolayer cultures for scanning and transmission electron microscopy (1986)
    Springer
    Methods in cell science 10 (1986), S. 239-244 
    Details
    ISSN: 1573-0603
    Keywords: monolayer culture ; scanning electron microscopy ; transmission electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A reliable method is described for processing substrate-dependent cells raised on culture-grade plastic for both scanning (SEM) and transmission electron microscopy (TEM). This technique allows collection of specimens for TEM and SEM from the same culture dish or flask. In this way it is possible to study the surface morphology (SEM) and thin section ultrastructure of cells from contiguous regions of a culture. Specific regions of a culture can be selected and processed so that specimens retain orientation throughout mounting or embedment and sectioning. The method is applicable both to confluent cultures as well as isolated colonies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01404484
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Use of the pH-sensitive dye BCECF to study pH regulation in cultured human kidney proximal tubule cells (1991)
    Springer
    Methods in cell science 13 (1991), S. 205-209 
    Details
    ISSN: 1573-0603
    Keywords: pH ; NH 4 + ; BCECF ; human kidney ; proximal tubule ; cell culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary This protocol describes the use of the pH-sensitive, intracellularly trapped dye 2′,7′-bis (2-carboxyethyl), 5 (and -6) carboxyfluorescein (BCECF), to characterize the pH regulating mechanisms in cultured human kidney proximal tubule cells. This is a reliable method for intracellular pH measurements and is applicable to single cells, cell suspensions, and confluent cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02388128
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Ultrastructure of ependymal cells of the shark median eminence (1974)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 178 (1974), S. 657-665 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The ultrastructure of ependymal cells of the median eminence in young Pacific blacktip reef sharks is described. Three major cell types have been observed. The first type is an irregularly-shaped, electron dense cell with many long microvilli and occasional cilia projecting into the ventricle. A second type contains dense cytoplasmic granules in sizes ranging from 1000-2000 Å, and may be morphologically equivalent to “liquor-contacting neurons” observed in ependymal linings elsewhere. The third type appears very similar to crown cells of saccus vasculosus of this and other species. Cells of the third type possess large, lipid-like inclusions, bulbous projections originating from basal bodies, and prominent smooth endoplasmic reticulum. The crown-like cells are compared to cells of saccus vasculosus of young and adult sharks, and their possible significance is discussed.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091780313
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    The lateral recess of the third ventricle in teleosts: An electron microscopic and golgi study (1976)
    Springer
    Cell & tissue research 166 (1976), S. 521-530 
    Details
    ISSN: 1432-0878
    Keywords: Ependyma ; Fish ; Third ventricle ; Hypothalamus ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ependyma lining the lateral recess of the third ventricle of the teleost inferior lobe has been studied by light and electron microscopy, including Golgi impregnation methods. As many as five different cell types appear to line the ventricle, but some of these may be similar cells in different stages of activity. One cell type contains small dense-cored vesicles and appears to have processes extending into deeper portions of the lobe. Golgi preparations reveal subependymal cells with apical processes extending to the ventricle and basal extensions which may reach the pial surface. The present observations are discussed in relation to similar studies in other fishes, amphibians and mammals. Possible functions for the various cells observed are suggested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225916
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    Paper (German National Licenses)
    Fulltext
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