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  • 1
    Electronic Resource
    Electronic Resource
    Growth and differentiation of human keratinocytes on extracellular matrix (1987)
    Springer
    Archives of dermatological research 279 (1987), S. 241-246 
    Details
    ISSN: 1432-069X
    Keywords: Cultures ; Epidermal cells ; Extracellular matrices ; Keratinocytes ; Pemphigus ; Pemphigoid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Extracellular matrices (ECM) have been reported to enhance epithelial cell attachment and proliferation as well as to induce differentiation in vitro. Since ECM components are physiological constituents of the dermoepidermal basement membrane, we studied the growth and differentiation of human keratinocytes on ECM in order to determine the benefits of culturing epidermal epithelial cells (keratinocytes) on reconstituted basement membranes. Dissaggregated epidermal cells were grown in primary and subcultures in liquid medium; the attachment of the cells was greatly enhanced by ECM and noted within the first few hours after seeding; cells formed small islets that reached confluence within 2–12 days depending upon the plating density and the type of culture (primary or passages). Histological and ultrastructural crosssections of the cultures clearly indicated that a multilayered epithelium can be obtained including a basal cell layer, several intermediate cell layers with cytoplasmic organelles, intermediate size filaments, desmosomes, and keratohyaline granules, and an upper layer of anucleated cells. Using immunofluorescence, both pemphigus and pemphigoid (basal membrane zone) antigens were expressed. The keratin pattern noted indicated that these epithelia differentiate and keratinize but do not express a complete program of keratinization, a finding usually noted when cells are grown submersed. These data show that ECM favor epidermal cell proliferation and differentiation and suggest that they may be used to obtain large amounts of epidermal equivalent suitable for grafting and/or in vitro studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00417322
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  • 2
    Electronic Resource
    Electronic Resource
    Pemphigus, pemphigoid, and epidermal upper-cytoplasmic antigens: Changes in expression in cultured human keratinocytes (1981)
    Springer
    Archives of dermatological research 271 (1981), S. 73-82 
    Details
    ISSN: 1432-069X
    Keywords: Antigens ; Culture ; Epidermal antigens ; Epidermal differentiation ; Epidermis ; Pemphigoid ; Pemphigus ; Antigen ; Kultur ; Epidermis ; Epidermiszellen ; Differenzierung ; Pemphigoid ; Pemphigus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Pemphigus- Pemphigoid- und Upper-Cytoplasmic (U-CYT)-Antigene der Epidermis wurden an in vitro-gezüchteten Epidermiszellen untersucht, um die Differenzierung der Epidermis immunologisch zu verfolgen. Menschliche Keratinocyten wurden in vitro ohne Feeder-Zellen, ohne Collagen oder dermales Gewebe kultiviert. Zellsuspensionen von trypsinierter Haut, Primär-, Sekundär- und Tertiärkulturen wurden mit dem indirekten Immunofluorescenzverfahren unter Verwendung von Seren von Patienten mit Pemphigus vulgaris, bullösem Pemphigoid sowie Seren mit Autoantikörpern gegen Keratinocyten-Cytoplasma getestet. Normale Seren und kultivierte normale menschliche Fibroblasten wie auch Melanomzellen dienten als Kontrolle. Pemphigus- sowie Pemphigoidantigen konnten in allen Epidermiszellkulturen nachgewiesen werden. U-CYT-Antigen wurde in 45–65% der trypsinierten Keratinocyten, jedoch nur in 5–10% der kultivierten und überhaupt nicht in subkultivierten Keratinocyten beobachtet. Die Bedeutung dieser Befunde für die Differenzierung der Epidermis in vitro wird diskutiert.
    Notes: Summary In an approach of epidermal differentiation, the expression of pemphigus, bullous pemphigoid, and upper-cytoplasmic epidermal antigens was studied in human keratinocytes in culture. The cells were cultured without feeder cells, dermal tissue, or collagen at an acid pH (5.6–5.8) similar to that of the surface of the skin in vivo. Cell suspensions from fresh trypsinized skin and primary, secondary, and tertiary cultures were tested by indirect immunofluorescence for the presence of each antigen using human sera from patients with pemphigus, bullous pemphigoid, and human sera with antibodies against upper-cytoplasmic antigens. Normal sera and cultured human normal fibroblasts and melanoma cells were used as controls. Pemphigus and pemphigoid antigens were found to be expressed, and synthesized by keratinocytes in vitro. The expression to upper-cytoplasmic antigens decreased with time in culture, and they were absent in secondary or tertiary cultures while expressed by 45–65% of cells prepared from fresh skin. Both upper-cytoplasmic and pemphigoid antigens can be used to type subpopulations of human epidermal cells; however, these findings suggest that epidermal differentiation in vitro differs from that which occurs in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00417390
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  • 3
    Electronic Resource
    Electronic Resource
    Immunohistochemistry in cutaneous pathology (1983)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of cutaneous pathology 10 (1983), S. 0 
    Details
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Since the first demonstration of skin antibodies in sera of pemphigus patients by IF (Beutner & Jordon 1964), the scope of skin diseases in which immunologic findings may be of interest has enormously enlarged and dermatology remains one of the leading clinical specialities applying IHC in both diagnosis and research. Immunohistological techniques and, to a lesser degree, immunocytological techniques, have been developed to include not only IF, but also immunoenzymatic methods. Even if not all immunological findings may be disease specific and diagnostic, immunohistological techniques have become a very useful tool for studying skin biopsies in connection with serological methods. Their importance could be increased with rapid development to a new field: the study of normal skin constituents; e.g. Ag, collagen, keratin, with the help of specific polyclonal and monoclonal antibodies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0560.1983.tb00313.x
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  • 4
    Electronic Resource
    Electronic Resource
    Re-expression of disease-characteristic features of non-bullous congenital ichthyosiform erythroderma (CIE) after graftig of the pathological keratinocyte cultures to athymic mice (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of cutaneous pathology 16 (1989), S. 0 
    Details
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Epidermal keratinocytes separated from skin lesions of non-bullous congenital ichthyosiform erythroderma were investigated in an attempt at experimental reproduction of this keratinization disorder. In vitro studies on growth and differentiation of pathological keratinocytes isolated from the influence of the host's dermal and humoral components were performed using the immersed epidermal cell culture technique. Ten to 25- day-old confluent and stratified cultures were examined by means of photonic and electron microscopy, and stained with various differentiation markers for indirect immunofluorescence studies. The cultured epidermis showed low-grade differentiation and no clear-cut evenly distributed signs of the original disease. Grafting on congenitally athymic nude mice allowed further differentiation of the epidermal sheets and re-expression of the histologic and ultrastructural features of non-bullous congenital ichthyosiform erythroderma. Thus, the purely epidermal origin of this particular form of autosomal recessive ichthyosis could be confirmed. Large amounts of pathological keratinocytes generated from small skin biopsies may be used for experimental purposes after grafting on several athymic animals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0560.1989.tb00001.x
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  • 5
    Electronic Resource
    Electronic Resource
    The physical state of water at low temperatures in plasma with different water contents as studied by differential thermal analysis and differential scanning calorimetry
    Amsterdam : Elsevier
    Cryobiology 12 (1975), S. 202-208 
    Details
    ISSN: 0011-2240
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0011-2240(75)90018-8
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  • 6
    Electronic Resource
    Electronic Resource
    Liability for nuclear accidents in Belgium from an interest group perspective
    Amsterdam : Elsevier
    International Review of Law and Economics 10 (1990), S. 241-254 
    Details
    ISSN: 0144-8188
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Law , Economics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0144-8188(90)90012-I
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  • 7
    Electronic Resource
    Electronic Resource
    Self-regulation of the professions in Belgium
    Amsterdam : Elsevier
    International Review of Law and Economics 11 (1991), S. 165-182 
    Details
    ISSN: 0144-8188
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Law , Economics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0144-8188(91)90017-8
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  • 8
    Electronic Resource
    Electronic Resource
    Location and reducibility of nickel(2+) ions in an X-type zeolite in the presence of lanthanum(3+) (1980)
    s.l. : American Chemical Society
    The @journal of physical chemistry 〈Washington, DC〉 84 (1980), S. 875-878 
    Details
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/j100445a017
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  • 9
    Electronic Resource
    Electronic Resource
    Cultured epithelia from junctional epidermolysis bullosa letalis keratinocytes express the main phenotypic characteristics of the disease (1990)
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 122 (1990), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Keratinocytes from a 1-week-old male infant with junctional epidermolysis bullosa letalis (JEBL) were grown in vitro and then grafted as multi-layered epithelia onto nude mice, to investigate whether the defect in the dermo-epidermal cohesiveness in the disease is of epidermal and not mesodermal origin. In culture, there was a birefringent ring of cells at the edges of the keratinocyte colonies and in places some cells looked as though they had been ejected from the periphery of the colony. At confluence, the multi-layered epithelia were easily detached from the culture flasks using only mechanical agitation. On microscopy the fully-differentiated epithelium on days 21, 30 and 40 after grafting sometimes showed blistering at the dermal-epidermal junction. No labelling was noted using a GB3 monoclonal antibody, that reacts with normal human keratinocytes in culture and with the dermo-epidermal basement membrane zone in normal skin. This indicates that the defect of JEBL may be reproduced in culture and also after grafting the cultured epithelia onto a wound without an epidermis. This suggests a possible role for the junctional structure recognized by GB3 in dermo-epidermal cohesiveness.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2133.1990.tb08259.x
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  • 10
    Electronic Resource
    Electronic Resource
    In vitro studies of epidermal antigen-presenting cells. The mixed skin lymphocyte reaction: an in vitro model for the generation of alloreactive cytotoxic T cells by human epidermal cells (1984)
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 111 (1984), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Langerhans cells and indeterminate cells are the unique antigen-presenting epidermal cells participating in human lympho-epidermal interactions. They bear class II HLA-DR molecules, can substitute for macrophages in antigen presentation, induce a T-cell proliferative response to antigens and haptens in sensitized donors, and are necessary for alloantigen T-cell activation and generation of alloreactive cytotoxic T cells in vitro. Indirect immunofluorescence and immunogold electron microscopy on class II positive epidermal cell enriched suspensions (panning, FAGS) indicated two populations of DR-positive epidermal cells: strongly DR-positive cells (25–30, 8% of positive epidermal cells) and faintly DR-positive cells, with a density of surface DR sites of respectively 5 × 105 and 1 × 105. Most Langerhans cells are among this second group while indeterminate cells are usually strongly DR-positive. OKT6-labelled cells were only typical Langerhans cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2133.1984.tb15577.x
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